The response of human dendritic cells to co-ligation of pattern-recognition receptors

Džopalić, Tanja; Rajkovic, Ivan; Dragicevic, Ana; Čolić, Miodrag

doi:10.1007/s12026-012-8279-5

Cited by 38 publications

(24 citation statements)

References 117 publications

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“…They recognize specific pathogen-associated molecular patterns on pathogens, such as lipids, carbohydrates, peptides, and nucleic acid structures (1,3). Intact microbial pathogens are composed of a variety of these pathogen-associated molecular patterns, which can simultaneously activate multiple PRRs and initiate a plethora of signaling programs that execute a first line of defense against infection (3,(6)(7)(8)(9).…”

mentioning

confidence: 99%

Antibody-Opsonized Bacteria Evoke an Inflammatory Dendritic Cell Phenotype and Polyfunctional Th Cells by Cross-Talk between TLRs and FcRs

Bakema

Tuk

Vliet

et al. 2015

The Journal of Immunology

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During secondary immune responses, Ab-opsonized bacteria are efficiently taken up via FcRs by dendritic cells. We now demonstrate that this process induces cross-talk between FcRs and TLRs, which results in synergistic release of several inflammatory cytokines, as well as altered lipid metabolite profiles. This altered inflammatory profile redirects Th1 polarization toward Th17 cell responses. Interestingly, GM-CSF–producing Th cells were synergistically evoked as well, which suggests the onset of polyfunctional Th17 cells. Synergistic cytokine release was dependent on activation via MyD88 and ITAM signaling pathways through TLRs and FcRs, respectively. Cytokine regulation occurred via transcription-dependent mechanisms for TNF-α and IL-23 and posttranscriptional mechanisms for caspase-1–dependent release of IL-1β. Furthermore, cross-talk between TLRs and FcRs was not restricted to dendritic cells. In conclusion, our results support that bacteria alone initiate fundamentally different immune responses compared with Ab-opsonized bacteria through the combined action of two classes of receptors and, ultimately, may refine new therapies for inflammatory diseases.

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mentioning

confidence: 99%

Antibody-Opsonized Bacteria Evoke an Inflammatory Dendritic Cell Phenotype and Polyfunctional Th Cells by Cross-Talk between TLRs and FcRs

Bakema

Tuk

Vliet

et al. 2015

The Journal of Immunology

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“…can promote antigen uptake as well as activating innate defence processes, either alone or in co-operation with C-type lectins or integrins [9,64,65]. Moreover, different DC receptors can promote uptake into different endocytic pathways.…”

Section: Dendritic Cell Sensingmentioning

confidence: 99%

Dendritic Cell Endocytosis Essential for Viruses and Vaccines

McCullough¹,

Sharma²

2017

Biology of Myelomonocytic Cells

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Protective immune defences are dependent upon critical roles played by dendritic cells (DCs), rendering them important targets for both vaccine delivery and virus infection. Studies in these areas led to successful development of targeted vaccine delivery, including synthetic virus-like particle (SVLP) and nanoparticulate RNA vaccines. A major consideration is DC endocytosis, whereby the different endocytic routes influencing the outcome. Rapid clathrin-mediated endocytosis likely favours degradative pathways. Slower processes such as macropinocytosis, caveolar endocytosis and retrograde transport to endoplasmic reticulum relate more to the processing rates leading to antigen presentation by DCs. These pathways are also influential in promoting the initiation of virus replication following infection. DC endocytosis of RNA viruses and RNA vaccines must lead to cytosolic translocation of the RNA for translation, relating to the process of antigen cross-presentation. One can learn from observations on both virus infections and cross-presentation for delivering RNA vaccines. Accordingly, recent advances in nanoparticulate delivery have been applied with self-amplifying replicon RNA (RepRNA), providing efficient delivery to DCs and promoting repliconencoded antigen translation. Through realising the important relationships between DC endocytic pathways and induction of immune responses, delivery of SVLP and RepRNA vaccines to DCs offers high value for the development of future synthetic vaccine platforms.

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“…Currently, methods for detecting PRRs are based in the use of stained antibodies specific for each PRR [55]. There are several PRRs, including TLRs, C-type lectin receptors (CLRs), cytoplasmic retinoic acid-inducible gene-I-like receptors (RLRs), nucleotide oligomerization domain-like receptors (NLRs) and others [56]. Upon recognition of antigens, especially via PRRs DCs undergo their maturation process.…”

Section: Targeting Dendritic Cellsmentioning

confidence: 99%

Dendritic Cells Interactions with the Immune System – Implications for Vaccine Development

Salvador¹,

Igartua²,

Pedraz³

et al. 2012

Cell Interaction

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The response of human dendritic cells to co-ligation of pattern-recognition receptors

Cited by 38 publications

References 117 publications

Antibody-Opsonized Bacteria Evoke an Inflammatory Dendritic Cell Phenotype and Polyfunctional Th Cells by Cross-Talk between TLRs and FcRs

Antibody-Opsonized Bacteria Evoke an Inflammatory Dendritic Cell Phenotype and Polyfunctional Th Cells by Cross-Talk between TLRs and FcRs

Dendritic Cell Endocytosis Essential for Viruses and Vaccines

Dendritic Cells Interactions with the Immune System – Implications for Vaccine Development

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