The Response of Dark Septate Endophytes (DSE) to Heavy Metals in Pure Culture

Ban, Yihui; Tang, Ming; Chen, Hui; Xu, Zhouying; Zhang, Haihan; Yang, Yurong

doi:10.1371/journal.pone.0047968

Cited by 106 publications

(75 citation statements)

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“…Comparable results were also obtained (Guelfi et al, 2003;Ban et al, 2012 andChakraborty et al, 2012).…”

Section: Pod Gr Pposupporting

confidence: 66%

An Investigation on Tolerance and Biosorption Potential of Aspergillus awamori ZU JQ 965830.1 to Cu(II)

2013

Egypt. J. Microbiol.

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HE MINIMUM inhibitory concentration (MIC) value of ……A.awamori for Cu(II) was 3100mg/l and a complete inhibition of biomass production was observed at 800mg/l concentration of Cu(II). Slight changes were observed by SEM investigation in Cu(II)-stressed biomass. Quantification of Cu(II) was performed by EDAX. Transmission electron microscopy investigation (TEM) confirmed the involvement of extracellular adsorption, intracellular penetration through the cell wall and vacuolation. Cu(II) stress induced noticeable changes in the activities of polyphenol oxidase(PPO), glutathione reductase (GR) and peroxidase (POD) and in the concentration of total antioxidant, soluble protein and thiol. High performance liquid chromatography analysis (HPLC) revealed that Cu(II) stress stimulated the production of oxalic acid .Maximum Cu(II) uptake capacity was achieved at pH 4.0, initial metal ion concentration 500mg/l and biomass dosage 1g/l. Maximum Cu(II) uptake capacities were reached after 180 min for live biomass and 30 min for dead biomass. Fourier transform infrared spectroscopy (FTIR) results gave an indicateion of chelation between oxygen-, nitrogen-, phosphorus-and especially sulphur-containing ligands of biomass with metal ions. X-ray diffraction analysis (XRD) revealed the presence of CuSO 4 .H 2 O in live and dead biomass. EDAX confirmed the occurrence of sulphur, oxygen and Cu(II) in the cell wall.Keywords: Aspergillus awamor;, Cu(II), Stress, Antioxidant enzymes, Biosorption.Several inorganic and organic compounds such as heavy metals, fuels and petroleum industry products cause soil and water contamination. For this reason, research focused toward better decontamination methods and the development of new technologies is imperative (Velásquez & Dussan, 2009).Remediation methods for heavy metals differ from those for organic compounds. As compared to organic compounds, metals are non-biodegradable (Gupta & Rastogi, 2008). Therefore, biomobilization is a valid concept in the management of metal pollution. Some metal ions, like Cu(II) and Zn(II) are essential for biological processes, but other nonessential metals like Cd(II) and Hg(II) are considered highly toxic elements to nearly all organisms even at low MANAL T. EL-SAYED Egypt. J. Microbiol. 48(2013) 24concentrations (Guelfi et al., 2003). However, Cu(II) is toxic to most organisms at elevated concentrations, largely through enzyme inhibition and oxidation of membrane components as a result of the ability of Cu(II) to generate toxic hydroxyl radicals (Melo et al., 2004). Membrane damage then leads to rapid leakage of ions and other low molecular weight compounds (Soares et al., 2003).Various techniques such as precipitation, coagulation, ion exchange, reverse osmosis, evaporation, filtration, electrochemical treatment and oxidation and reduction have been employed to remove toxic heavy metals from industrial wastewaters. Most of these techniques are ineffective or extremely expensive in terms of energy and reagent consumption, especially when concentrations of diss...

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“…Comparable results were also obtained (Guelfi et al, 2003;Ban et al, 2012 andChakraborty et al, 2012).…”

Section: Pod Gr Pposupporting

confidence: 66%

An Investigation on Tolerance and Biosorption Potential of Aspergillus awamori ZU JQ 965830.1 to Cu(II)

2013

Egypt. J. Microbiol.

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“…Todavia, sabe-se que os efeitos tanto dos FMA como dos DSE para as plantas vão além dos nutricionais. Os DSE, por exemplo, conferem uma série de benefícios indiretos para seus hospedeiros, como resistência contra agentes patogênicos prejudiciais as raízes e herbívoros (REININGER; SIEBER, 2012; TELLENBACH; SIEBER, 2012), além de garantir a sobrevivência das plantas em condições estressantes (BAN et al, 2012).…”

Section: Resultsunclassified

“…Além do efeito nutricional, os DSE conferem à planta proteção contra patógenos (RODRIGUEZ et al, 2009;REININGER; e adaptação às condições extremas do solo (SCHMIDT et al, 2008), tais como solos contaminados por metais pesados (BAN et al, 2012;ZHAO et al, 2015), com estresse hídrico e salino (WALLER et al, 2005) e com elevado teor de acidez . Também foi observado que os DSE podem coexistir com fungos micorrízicos arbusculares e produzir metabólitos capazes de aumentar a germinação, o crescimento e a ramificação de hifas desses fungos, beneficiando a planta hospedeira de forma indireta .…”

Section: Introductionunclassified

“…Em geral, elevada disponibilidade de nutrientes no solo e baixos valores de pH apresentam uma correlação negativa com os FMA (ENTRY, 2002;LEKBERG et al, 2007;GRILLI et al, 2013). Entretanto, ainda muito pouco se conhece sobre a influência dos parâmetros do solo na comunidade dos DSE, apenas sabe-se que condições estressantes do solo, como elevada acidez e presença de metais pesados podem favorecer esses endófítos BAN et al, 2012).…”

Section: Introductionunclassified

“…Atualmente, pesquisas sobre os DSE têm recebido cada vez mais interesse, entretanto a maioria dos estudos trata apenas da ocorrência desse grupo de fungos nos mais diversos ambientes (MARINS, 2009;PEREIRA et al, 2011;MASSENSSINI et al, 2014). De fato, os DSE são abundantes e têm sido relatados em diversos hospedeiros, compreendendo mais de 600 espécies de plantas (JUMPPONEN;JUMPPONEN et al, 2001; e em ambientes com condições extremas, como solos contaminados por metais pesados (BAN et al, 2012;ZHAO et al, 2015), com estresse hídrico , salino (WALLER et al, 2005) e com elevada acidez . Sabe-se que a resistência às condições inóspitas são dadas pela presença de melanina em suas hifas (JUMPPONEN; TRAPPE, 1998).…”

Section: Introductionunclassified

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Fungos micorrízicos arbusculares e endofíticos dark septate em áreas de Mata Atlântica em um gradiente altitudinal

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Cellular Response to Cu‐ and Zn‐Induced Oxidative Stress in Aspergillus fumigatus Isolated From Polluted Soils in Bulgaria

Krumova

Kostadinova

Miteva‐Staleva

et al. 2016

CLEAN Soil Air Water

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The fungal strain Aspergillus fumigatus 3 2 isolated from the tailings pond at the copper mine "Vlaykov vruh," Bulgaria, showed high tolerance to Cu 2þ and Zn 2þ . This study was designed to explore the relationship between Cu 2þ and Zn 2þ tolerance and cellular response to oxidative stress. The model strain was identified to species level by morphological and molecular methods. Fungal cultures were exposed to enhanced concentrations of metal ions. The effect of Cu 2þ and Zn 2þ was evaluated by the changes in fungal growth and morphology, the level of oxidative stress biomarkers, and the antioxidant activities of enzymes such as superoxide dismutase (SOD) and catalase (CAT). Two different cellular responses occurred: The concentrations of up to 500 mg/mL caused enhanced levels of oxidative stress biomarkers (glycogen and trehalose accumulation and oxidatively damaged protein content), as well as an increase in SOD and CAT activities. The treatment with concentrations from 500 to 2000 mg/mL led to enhanced glycogen consumption, accelerated proteolysis, and a decrease in SOD and CAT activities. The present results provide additional information about the participation of oxidative stress and antioxidant defense in enhanced tolerance of fungi isolated from metal-polluted soils. Probably, survival at extremely high concentrations also requires the participation of other defense mechanisms. Furthermore, the enhanced understanding of these processes will provide essential tools for efficient remediation practices.

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The Response of Dark Septate Endophytes (DSE) to Heavy Metals in Pure Culture

Cited by 106 publications

References 66 publications

An Investigation on Tolerance and Biosorption Potential of Aspergillus awamori ZU JQ 965830.1 to Cu(II)

An Investigation on Tolerance and Biosorption Potential of Aspergillus awamori ZU JQ 965830.1 to Cu(II)

Fungos micorrízicos arbusculares e endofíticos dark septate em áreas de Mata Atlântica em um gradiente altitudinal

Cellular Response to Cu‐ and Zn‐Induced Oxidative Stress in Aspergillus fumigatus Isolated From Polluted Soils in Bulgaria

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