The respiratory chain and the oxidative phosphorylation of rat brain mitochondria

Voss, Dinor O.; Campello, Annibal P.; Bacila, Metry

doi:10.1016/0006-291x(61)90253-4

Cited by 120 publications

(47 citation statements)

References 3 publications

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“…Los experimentos fueron realizados bajo agitación constante en cámara termostatizada a 28°C (24). El consumo de oxígeno por las mitocondrias intactas fue determinado polarográficamente por medio de un oxígrafo, utilizando un electrodo tipo Clark, acoplado a un registrador.…”

Section: Determinación Del Consumo De Oxígenounclassified

“…Objective: In this study the effect of linalool on the bioenergetics of isolated rat liver mitochondria was evaluated. Methods: Linalool in concentrations between (5-100μg/mL) was evaluated in isolated mitochondria of male albino Wistar rats, according to the method described by Voss et al (1961). Initially, the oxygen consumption by intact mitochondria was determined by polarographic method using an oxygraph.…”

Section: Introduccciónmentioning

confidence: 99%

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Linalool effect on mitochondrial bioenergetics rat liver

Bueno-Duarte

Méndez‐Sánchez

2015

Vitae

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RESUMENAntecedentes: El linalool (2,6-dimetil-2,7-octadien-6-ol) es un monoterpeno presente en más de 200 tipos de plantas, y constituye el componente mayoritario de varios aceites esenciales. El linalool posee una amplia variedad de potenciales propiedades farmacológicas; que van desde propiedades antisépticas, ansiolíticas, anti-inflamatorias, antifúngicas, antimicrobianas y proapotóticas en diferentes líneas de cé-lulas tumorales. Sin embargo, su principal uso está relacionado como una molécula odorante o fragancia en productos de uso tópico en productos cosméticos y en la industria del perfume. Objetivo: En este estudio se evaluó el efecto del linalool sobre la bioenergética de mitocondrias aisladas de hígado de rata. Métodos: El linalool en concentraciones entre (5-100μg/mL) fue evaluado en mitocondrias aisladas de ratas machos albinas raza Wistar, de acuerdo al método descrito por Voss et al (1961). Inicialmente, se determinó el consumo de oxígeno en mitocondrias intactas mediante un método polarográfico por medio de un oxígrafo. Posteriormente, se determinó el efecto del compuesto sobre las enzimas de la cadena respiratoria mitocondrial usando métodos polarográficos y espectrofotométricos. Resultados: Los resultados obtenidos indican que el linalool en las concentraciones de 5, 10, 50 y 100μg/mL, afecta la velocidad de consumo de oxígeno en el estado 3 y 4 de mitocondrias energizadas con glutamato-malato y succinato. Sin embargo, el efecto inhibitorio es mayor al usar glutamato-malato como sustrato. Por su parte, el análisis de la actividad de las enzimas de la cadena respiratoria mitocondrial mostró que el linalool (50μg/mL) inhibe los complejos I, III y la actividad ATPasa. Conclusiones: El linalool inhibe el complejo I, complejo III y la ATPasa, esta inhibición podría aumentar la generación de especies reactivas de oxígeno.Palabras clave: Linalool, mitocondria, enzimas, consumo de oxígeno. ABSTRACTBacground: The linalool (2,6-dimethyl-2 ,7-octadiene-6-ol) is a monoterpene present in more than 200 types of plants, and it is the major component of several essential oils. The linalool has a wide range of potential pharmacological properties, such as antiseptic, anxiolytic, anti-inflammatory, antifungal, antimicrobial and proapoptotic in different tumor cell lines. However, its primary use is associated as

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Section: Determinación Del Consumo De Oxígenounclassified

Section: Introduccciónmentioning

confidence: 99%

Linalool effect on mitochondrial bioenergetics rat liver

Bueno-Duarte

Méndez‐Sánchez

2015

Vitae

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“…For the swelling experiments, mitochondria were isolated from rat liver by differential centrifugation, according to the procedures described by Voss et al, 20 with modi®cations, using an extraction medium consisting of 330.0 mmol l À1 sucrose, 1.0 mmol l À1 Tris-HCl (pH 7.5) and 1.0 mmol l À1 EDTA. 21 In all cases, the mitochondrial pellet was washed twice with the extraction medium, the last pellet being washed and suspended in the same medium free of EDTA.…”

Section: Isolation Of Mitochondriamentioning

confidence: 99%

Interference of MI‐D, a new mesoionic compound, on artificial and native membranes

Cadena

Carnieri

Echevarrı́a

et al. 2001

Cell Biochemistry & Function

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MI-D (4-phenyl-5-(4-nitrocinnamoyl)-1,3,4-thiadiazolium-2-phenylamine chloride), a new mesoionic compound, decreased the rate of swelling induced by valinomycin-K+, as well as induced swelling in the presence of nigericin-K+. Shrinkage was also affected, suggesting interference with the inner mitochondrial membrane, which would affect both fluidity and elasticity. Fluorescence polarization of DPH and DPH-PA, probing the core and outer regions respectively, of the DMPC and native membranes, indicated that MI-D shifts the midpoint of phase transition to higher values and orders of the fluid phase. These alterations in membrane fluidity are thus related to MI-D effects on the energy-linked functions of mitochondria.

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“…Rat liver mitochondria were prepared according to Voss et al (1961) with slight modifications. Male Wistar rats were sacrificed by decapitation; liver was immediately removed, sliced in the extraction medium and homogenized three times with a Potter-Elvehjem homogenizer.…”

Section: Preparation Of Rat Liver Mitochondriamentioning

confidence: 99%

Effects of natural flavones on membrane properties and citotoxicity of HeLa cells

Herrerias¹,

Oliveira²,

Belem³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Efeitos de flavonas naturais em propriedades de membranas e em citotoxicidade de células HeLa". O objetivo deste estudo foi avaliar se eupafolina e hispidulina, flavonas extraídas do Eupatorium littorale Cabrera, Asteraceae, possuíam a capacidade de alterar propriedades das membranas biológicas e promover efeitos citotóxicos. Eupafolina (50-200 µM) reduziu em aproximadamente 30% a velocidade e amplitude do inchamento mitocondrial induzido por valinomicina e 60-100% o inchamento mitocondrial dependente de substrato. Além disso, eupafolina na dose de 200 µM reduziu a transição de permeabilidade mitocondrial em 35% entretanto, a hispidulina não alterou este parâmetro em todas as doses testadas. A avaliação da transição de fase dos lipossomas de DMPC com a sonda DPH demonstrou que ambas as flavonas afetam a fase gel e fluida. Quando lipossomas de membranas mitocondriais e a sonda DPH-PA foram utilizados, houve aumento da polarização de fluorescência promovido pela hispidulina. Eupafolina e hispidulina, na dose de 100 µM, promoveram 40% de redução da viabilidade de células HeLa em 24 h. Nossos resultados sugerem que eupafolina e hispidulina têm efeitos citotóxicos que podem ser explicados em parte pelas alterações promovidas por estas flavonas sobre propriedades de membranas biológicas e sobre a bioenergética mitocondrial.Unitermos: Eupafolina, hispidulina, células HeLa, inchamento mitocondrial. ABSTRACT:The aim of this study was to determine whether eupafolin and hispidulin, flavones extracted from Eupatorium littorale Cabrera, Asteraceae, have the ability to change properties of biological membranes and promote cytotoxic effects. Eupafolin (50-200 µM) decreased approximately 30% the rate and total amplitude of valinomycin induced swelling and 60-100% the energy-dependent mitochondrial swelling. Moreover, eupafolin (200 µM) reduced 35% the mitochondrial permeability transition, and hispidulin did not change this parameter in any of the doses tested. The evaluation of phase transition of DMPC liposomes with the probe DPH demonstrated that hispidulin and eupafolin affect gel and fluid phase. With mitochondrial membrane as model, hispidulin increased the polarization of fluorescence when used DPH-PA probe. Eupafolin and hispidulin (100 µM) promoted a reduction of 40% in cellular viability of HeLa cells in 24 h. Our results suggest that eupafolin and hispidulin have cytotoxic effects that can be explained, in part, by alterations promoted on biological membranes properties and mitochondrial bioenergetics.

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The respiratory chain and the oxidative phosphorylation of rat brain mitochondria

Cited by 120 publications

References 3 publications

Linalool effect on mitochondrial bioenergetics rat liver

Linalool effect on mitochondrial bioenergetics rat liver

Interference of MI‐D, a new mesoionic compound, on artificial and native membranes

Effects of natural flavones on membrane properties and citotoxicity of HeLa cells

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