The respiration of Mycobacterium phlei

The Effect of Rotation Aeration on the Growth of Mycobacterium Tuberculosis

“…This is consistent with reports on the intensity of oxygen consumption of tubercle bacilli at different stages of growth when in the presence of glucose ; in the first stage of lag phase growth there is a rapid uptake of oxygen until the glucose disappears from the medium (16,30,31 Youman's and Youmans(10) reported a method for the determination of the growth rate of Mycobacterium tuberculosis in a liquid medium, which was based upon the determination of the time necessary for small inocula of tubercle bacilli to grow until they comprised a certain standard mass. This is consistent with reports on the intensity of oxygen consumption of tubercle bacilli at different stages of growth when in the presence of glucose ; in the first stage of lag phase growth there is a rapid uptake of oxygen until the glucose disappears from the medium (16,30,31 Youman's and Youmans(10) reported a method for the determination of the growth rate of Mycobacterium tuberculosis in a liquid medium, which was based upon the determination of the time necessary for small inocula of tubercle bacilli to grow until they comprised a certain standard mass.…”

The Intermediary Metabolism of the Mycobacteria

“…This has been confirmed by Ebina and Nakamura (29) who examined human, bovine and avian strains of tubercle bacili as well as M. phlei and M. megmatis and found a very low anaerobic glycolysis (Q4r2 = 0.2 to 2.2). Indeed, it would appear that washed suspensions have no anaerobic metabolism, since glucose, pyruvate, lactate and fatty acids can be recovered almost quantitatively in anaerobic experiments (32,60). Further, there is no aerobic glycolysis (29).…”

Studies on the Metabolism of Mycobacterium Tuberculosis Iv

“…In contrast to the findings obtained from growth studies, certain fatty acids have been found to stimulate the respiration of mycobacteria (Loebel et al, 1933;Cutinelli, 1940;Edson and Hunter, 1947;Saz, 1949;Lindsay et al, 1950;Oginsky et al, 1950;Geronimus and Birkeland, 1951). Bernheim (1941) observed that the oxygen uptake was increased more by the sodium salts of the lower molecular weight fatty acids than by the sodium salts of the higher molecular weight fatty acids, and that the amount of oxygen uptake was proportional to the concentration of the acid.…”

“…In the present study, the fatty acids, Cs and Clo (caprylic and capric acids), stimulated the growth of the organisms more than any of the other fatty acids examined. Formic acid did not support growth, nor did it stimulate the oxygen uptake of the H37 strain, the bovine B1 strain (Bernheim, 1941), or M. ranae (Edson and Hunter, 1947).…”

“…The fatty acids of lower molecular weight stimulated the growth of mycobacteria more than the fatty acids of high molecular weight. Like- wise Bernheim (1941), Cutinelli (1941), and Edson and Hunter (1947) found that the respiration of mycobacteria was stimulated more by the lower fatty acids than by the higher fatty acids. Cutinelli (1940) observed that the fatty acids from C7 to Cui were oxidized most rapidly.…”