The respiration of cells and mitochondria of porin deficient yeast mutants is coupled

Cytoprotective activity of minocycline includes improvement of mitochondrial coupling: the importance of minocycline concentration and the presence of VDAC

“…On the other hand, a maximal rate of oxygen uptake (uncoupled state, state U) can be induced by FCCP, collapsing the inner membrane potential. Calculations of both native and state 4 respiration percentage of maximal respiration can provide information about the degree of coupling in cell respiration (Michejda et al 1990). A representative trace applied for further calculations is shown in Fig.…”

“…Ethanol (diffusing freely across biological membranes) was applied as the respiratory substrate known to be oxidized by S. cerevisiae mitochondria (De Vries and Marres 1987). The real state 4 was enforced by tributylin (TBT), known to inhibit ATP synthase and to act effectively in the case of S. cerevisiae cells (Michejda et al 1990). As shown in Fig.…”

“…For measurements of wild type and Δpor1 cell respiration, the cells were washed three times in 50 mM potassium citrate buffer, pH 4.5 and then used for oxygen uptake measurements (Michejda et al 1990). …”

“…To obtain actual (native) respiration (fluctuating between state 4 and state 3), 10 mM ethanol was added as the respiratory substrate. State 4 and state U were induced by addition of tributylin (TBT) and FCCP, respectively (Michejda et al 1990). TBT inhibits mitochondrial ATPase, but when added in too high concentrations it can cause uncoupling as a side effect.…”

“…The yeast Saccharomyces cerevisiae has been proven to be a very useful model in studies concerning the status of energy coupling in intact cells and isolated mitochondria (Michejda et al 1990). Moreover, evolutionary proximity of animals and fungi is reflected in the presence of homologous genes and proteins in S. cerevisiae and humans.…”