The Resistance of Immature Erythrocytes to Heat *

Isaacs, Raphael; Brock, Benjamin L.; Minot, George R.

doi:10.1172/jci100022

Cited by 11 publications

(5 citation statements)

References 1 publication

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“…Another aspect of haematological change was manifested by the significant increase in osmotic fragility of human red cells found after the subjects were briefly exposed to severe heat stress (Abramson & Hyde, 1967). Similar patterns were demonstrated after in uitro heating of red cells (Isaacs et al, 1925) and also in experimental pyrexia induced in rabbit and man (Karle, 1969).…”

supporting

confidence: 75%

Haematological Changes in Heat‐Acclimated Golden Hamsters

Meyerstein¹,

Cassuto²

1970

Br J Haematol

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Summary. Haematological changes were investigated in heat‐acclimated hamsters. Haemoglobin, packed‐cell volume, and red‐cell counts were not affected by heat acclimation. Glucose uptake and osmotic fragility in the red blood cells of these animals were increased. The red cell ATP level was lower in heat‐acclimated than in control hamsters. No change was observed in the density distribution of the red cell population in heat‐acclimated hamsters. The resemblance of these cells to the red cells of hereditary spherocytosis and pyruvate‐kinase deficiency is discussed.

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supporting

confidence: 75%

Haematological Changes in Heat‐Acclimated Golden Hamsters

Meyerstein¹,

Cassuto²

1970

Br J Haematol

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“…The fragility was normal in only 1 of these patients (Case 11), whose burn involved 20 per cent of the body area. In the other 3 patients, the osmotic fragility was increased as follows: slightly in Case 12. burned 65 per cent; moderately in Case 14, burned 15 solution and thus, presumably, were susceptible to osmotic hemolysis in the patient's own isotonic blood plasma. The progressive disappearance and probable destruction of the red cells with abnormally increased osmotic fragility are shown for Cases 4 and 14 in Figures 3 and 4, respectively.…”

Section: Changes In Red Cellsmentioning

confidence: 95%

Studies on the Destruction of Red Blood Cells

et al. 1943

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“…Below 50°C. there may be shrinking of the cell, resulting in a decrease in cell volume, due to the reduction in osmotic pressure (20)(21)(22)(23)(24)(25). At 50°C.…”

Section: Discussionmentioning

confidence: 99%

“…At 50°C. fragmentation has begun or is in process (20,(26)(27)(28) and it would appear that as the new cells or fragments are being formed they are more fragile upon subjection to sonic oscillation. At 53°C.…”

Section: Discussionmentioning

confidence: 99%

Experimental Variations in Sonic Fragility of Red Cells

Guillet

Fowler

1954

Journal of General Physiology

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The reaction of red cells to intense sonic and ultrssonic vibrations was studied by many workers following the development of the piezoelectric crystal and the magnetostriction oscillator. The original observers (1-10) attxibuted the hemolysis to rapid fluid movement as a result of cavitation. Slight physicalchemical changes in the cell were shown following subhemolyfic exposures to ultrasonic vibrations, such as a small inhibition of cell respiration (11), an increase in viscosity and a decrease in cell volume (12), a slight decrease in the oxygen-combining power at high oxygen pressures (13), and an unchanged resistance to hypotonic saline (14,15,12). The suspension medium has an effect upon the rate of hemolysis (10).Williams and Gaines (16) and Chambers and Gaines (17) using a son/c oscillator observed the laking of blood cells suspended in isotonic saline. By hemocytometer counts taken at regular intervals during the treatment, the following relationship was determined: N ~ Noe-~', N being the number of uncytolyzed cells at time, t, and No, the original number of cells. The hemolysis was explained as the result of rapid alterations of tension and compression produced in the surrounding medium by the vibrations.In the following experiments a further study of the kinetics of hemolysis by sonic oscillation was made. Also, the changes in sonic fragility brought about as a result of pretreatment of the cells were investigated. MethodBlood was drawn by venipuncture from normal healthy subjects and used immediately for experimentation. The anticoagulant used was a two-to-one mixture of ammonium and potassium oxalate. Hemolysis was induced by a Raytheon 50 watt, 9 kc. magnetostriction oscillator having a laminated nickel rod attached to the diaphragm at the base of a stainless steal cup. One cc. of blood was diinted with 25 cc. of saline, and this was delivered directly into the cup and subjected to sonic vibration for varying periods of time. The suspension was centrifuged following osciUafion and

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The Resistance of Immature Erythrocytes to Heat *

Cited by 11 publications

References 1 publication

Haematological Changes in Heat‐Acclimated Golden Hamsters

Haematological Changes in Heat‐Acclimated Golden Hamsters

Studies on the Destruction of Red Blood Cells

Experimental Variations in Sonic Fragility of Red Cells

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