The Resilience of Microbial Community Involved in Coking Wastewater Treatment System

Abstract

Biological treatment processes are the most important step for removing various nutrient matters due to their high removal efficiency and low operational cost. The activated sludge technology was used extensively in coking wastewater biological treatment processes where a complex prokaryotic microbial consortium played an important role in the processes of oxygen-depleting organics, toxics and nutrients removal and preventing discharge of pathogens. Thus, a thorough knowledge of the ecology of the coking wastewater activated sludge communities is required to improve system operation and stability. Therefore, there is necessary to reveal the resilience of microbial community involved in coking wastewater treatment system. Keywords: Biological treatment processes; Nutrient matters; Wastewater; Pathogens; Microbial community Mini ReviewIn this report, we investigated a full-scale coking wastewater treatment system with two paralleled units to see the resilience of microbial community. The full-scale CWW treatment system, an ongoing three-phase fluidized bed biological coking wastewater treatment system in Shaoguan, Guangdong Province of China, was run with south and north two paralleled units [1][2][3][4]. These two paralleled systems have been operating for more than years at daily capacity of approximately 1680 m 3 ± 80 m 3 . The total hydraulic retention time (HRT) was 46 h. These two paralleled systems were able to maintain a stable process performance during operation and also the final effluent meet the national stringent effluent standards. The coking wastewater activated sludge of anaerobic process was collected from the corresponding bioreactor of two paralleled systems in March 2016. Illumina MiSeq high throughput sequencing technology was performed to reveal the relative abundance and diversity of microbiology in these two parallel systems. Since coking wastewater is usually regarded as an extreme environment with high toxicity and refractoriness, the microorganisms involved should be quite different from those in domestic wastewater [5]. But the microbial community which is responsible for the cooking wastewater treatment process is still significantly different?The composition of the PCR products of V4 region of 16S rRNA genes was determined by MiSeq Sequencing. After MiSeq Sequencing. The raw data were processed and analyzed following the pipelines of Mothur and QIIME Raw data were denoised using the Mothur implementation of PyroNoise algorithm. Chimeric sequences were identified and removed using UCHIME with de novo method. Quality sequences were assigned to samples according to their unique 8-bp barcodes. Operational taxonomic units (OTUs, defined at the 97% sequence similarity level) were picked using average neighbor method after the Needleman alignment and a single-linkage precluster procedure. Taxonomy classification was identified using RDP Classifier at the 80% confidence threshold by default. Alpha-(number of OTUs, Chao 1, and Simpson and Shannon indices) diversity were statistically ...

Diversity and functional prediction of microbial communities involved in the first aerobic bioreactor of coking wastewater treatment system

“…Most abundant genera showed no significant difference between the two subsystems ( p > 0.05). The minor differences may be attributed to the elasticity of the biological diversity, but will not affect their function [ 50 ].…”

Biological treatment of coke plant effluents: from a microbiological perspective

“…Recent advances in molecular biology (e.g., the introduction of high-throughput DNA sequencing methods) aided to decipher the detailed taxonomic structure of microbial communities in the bioreactors. Studies revealed that members of the phylum Proteobacteria are the most abundant microorganisms in the bioreactors, contribution of the phyla Bacteroidetes, Acidobacteria, Firmicutes, Actinobacteria and Planctomycetes is also remarkable in most cases, and archaea represent a negligible fraction of the bioreactor communities (Jia et al 2016;Ma et al 2015a, b;Meng et al 2016;Xu et al 2016;Zhu et al 2015Zhu et al , 2017Ziembińska-Buczyńska et al 2019).…”

“…Although the removal of phenolics is usually carried out by bacteria, some fungi (Aspergillus sp., Fusarium sp., thiocyanate degradation, denitrification Banerjee (1996), Felföldi et al (2010), Katayama et al (1992), Ma et al (2015a, b), Meng et al (2016), Raper et al (2019b), Xu et al (2016), Zhu et al (2015Zhu et al ( , 2017 Penicillium sp., Graphium sp.) were also isolated from stainless steel industry wastewaters that are capable of phenol degradation (Santos and Linardi 2004) or were reported to be able to degrade phenolic compounds present in coking wastewater (Lu et al 2009).…”

“…Although there are fixed-film systems available and the presence of extracellular polymeric substances in biofilms may prevent bacteria from the harmful effect of contaminants (Ziembińska-Buczyńska et al 2019), biological treatment of coking wastewater is mainly carried out using activated sludge-based technologies (Zhu et al 2017).…”