The Research of Small Molecule Soybean Polypeptide Precipitated by TCA

Abstract

Abstract. Trichloroacetic acid (tca) makes macromolecular peptides expose more hydrophobic groups and have precipitation effect. It's widely used in the desalination and concentration of protein and peptide. This experiment use the alkaline protease enzymolysis the soybean protein isolated and makes the products as raw material, then do the high performance liquid chromatography (hplc) analysis of tca precipitation before and after enzymolysis liquid. Macromolecular peptidesore more than 10000 completely precipitated. Results show that the precipitation rate of more than 3000 u reached 62.01%, the molecular weight of 3000-1000 u is about 37.71%.When the molecular weight is less than 1000 u, the polypeptide precipitation rate drops to 22.51%. For the peak belonging 420 u, the main reason is that tca have strong absorption peak and a small amount of impurity absorption peak.