The RESA-2 gene of Plasmodium falciparum is transcribed in several independent isolates

Vazeux, Gilles; Scanf, Cécile Le; Fandeur, Thierry

doi:10.1128/iai.61.10.4469-4472.1993

Cited by 19 publications

(6 citation statements)

References 18 publications

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“…Ring-infected erythrocyte surface antigen 2, RESA2 (PF11_0512) was first described as a pseudogene based on the presence of an internal stop codon ( Cappai et al, 1992 ). Since then, transcription of this gene has been demonstrated both in vivo ( Vazeux et al, 1993 ) and in vitro ( Bozdech et al, 2003 ). RESA-2 is transcribed but poorly translated in rings, early trophozoites and merozoites (log 2 TE −3.2, −2.7, −2.9, respectively).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide regulatory dynamics of translation in the Plasmodium falciparum asexual blood stages

Caro

Ahyong

Betegon

et al. 2014

eLife

122

179

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The characterization of the transcriptome and proteome of Plasmodium falciparum has been a tremendous resource for the understanding of the molecular physiology of this parasite. However, the translational dynamics that link steady-state mRNA with protein levels are not well understood. In this study, we bridge this disconnect by measuring genome-wide translation using ribosome profiling, through five stages of the P. falciparum blood phase developmental cycle. Our findings show that transcription and translation are tightly coupled, with overt translational control occurring for less than 10% of the transcriptome. Translationally regulated genes are predominantly associated with merozoite egress functions. We systematically define mRNA 5′ leader sequences, and 3′ UTRs, as well as antisense transcripts, along with ribosome occupancy for each, and establish that accumulation of ribosomes on 5′ leaders is a common transcript feature. This work represents the highest resolution and broadest portrait of gene expression and translation to date for this medically important parasite.DOI: http://dx.doi.org/10.7554/eLife.04106.001

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Section: Resultsmentioning

confidence: 99%

Genome-wide regulatory dynamics of translation in the Plasmodium falciparum asexual blood stages

Caro

Ahyong

Betegon

et al. 2014

eLife

122

179

View full text Add to dashboard Cite

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“…A number of genes related to RESA have been identified, some of which contain the DnaJ motif (Cappai et al, 1992;Vazeux et al, 1993;Hinterberg et al, 1994a;Gardner, M.J. et al, 1998). The function of these various RESArelated proteins is currently unknown.…”

Section: Resa (Ring-infected Erythrocyte Surface Antigen)mentioning

confidence: 99%

The malaria-infected red blood cell: Structural and functional changes

Cooke

Mohandas

Coppel

2001

Advances in Parasitology

184

149

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The asexual stage of malaria parasites of the genus Plasmodium invade red blood cells of various species including humans. After parasite invasion, red blood cells progressively acquire a new set of properties and are converted into more typical, although still simpler, eukaryotic cells by the appearance of new structures in the red blood cell cytoplasm, and new proteins at the red blood cell membrane skeleton. The red blood cell undergoes striking morphological alterations and its rheological properties are considerably altered, manifesting as red blood cells with increased membrane rigidity, reduced deformability and increased adhesiveness for a number of other cells including the vascular endothelium. Elucidation of the structural changes in the red blood cell induced by parasite invasion and maturation and an understanding of the accompanying functional alterations have the ability to considerably extend our knowledge of structure-function relationships in the normal red blood cell. Furthermore, interference with these interactions may lead to previously unsuspected means of reducing parasite virulence and may lead to the development of novel antimalarial therapeutics.

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“…The other clones which were not recognized by human antibodies but reacted with reagents raised to or eluted from infected erythrocyte membranes were classified in reactivity groups 2 to 4, according to their specificities. Clones 16,32,22,24,17,41, and 45, which reacted with the rabbit serum and with the antibodies eluted from the surfaces of R-infected erythrocytes and reacted faintly or not at all with antibodies eluted from the surfaces of O-infected erythrocytes, formed reactivity group 2. Clones 10 and 13, which reacted with the antibodies eluted from the surfaces of Rinfected erythrocytes but failed to react with any of the other reagents tested were classified in group 3.…”

Section: Resultsmentioning

confidence: 99%

Novel Target Antigens of the Variant-Specific Immune Response toPlasmodium falciparumIdentified by Differential Screening of an Expression Library

et al. 1999

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A primary infection by the Plasmodium falciparum Palo Alto O and R antigenic variants induces a variantspecific immunity in the Saimiri sciureus monkey. We have shown that these variants express distinct PfEMP1 antigens and differ in their levels of expression of additional antigens, including two conserved erythrocyte membrane-associated proteins, HRP1 and PfEMP3. To identify the antigens eliciting a variant-specific response, we conducted a differential screening of a gt11 library with variant-specific sera. We report here the analysis of the 46 anti-R-specific clones. Two specific targets of the anti-R response were identified: (i) PfEMP3, suggesting that immunogenicity of this antigen is modulated by its relative abundance in different variants, and (ii) Asn-rich motifs. Most anti-R-specific clones, derived from so-far-undescribed genes, were detected by a cross-reaction on poly(Asn) stretches, as indicated by elimination of the signal after absorption on Asn-rich sequences. Reverse transcription-PCR (RT-PCR) showed that expression of the gene defined by clone 13 was R specific. Pepscan analysis of clone 13 identified three Asn-rich polypeptides and one unique peptide reacting specifically with antibodies eluted from the R-infected erythrocyte surface. Antisera raised to the unique peptide reacted with an R-specific protein. Attempts to demonstrate that clone 13 was derived from a var gene by using PCRs combining clone 13 and var-derived primers were unsuccessful. The var genes expressed by O and R parasites were identified not by this strategy but by RT-PCR with var-specific primers. This work has provided novel insights into immunity to antigenic variants and has identified a novel gene switched on during antigenic variation.

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The RESA-2 gene of Plasmodium falciparum is transcribed in several independent isolates

Cited by 19 publications

References 18 publications

Genome-wide regulatory dynamics of translation in the Plasmodium falciparum asexual blood stages

Genome-wide regulatory dynamics of translation in the Plasmodium falciparum asexual blood stages

The malaria-infected red blood cell: Structural and functional changes

Novel Target Antigens of the Variant-Specific Immune Response toPlasmodium falciparumIdentified by Differential Screening of an Expression Library

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