The serine/threonine kinase mammalian/mechanistic target of rapamycin (mTOR) integrates various environmental cues such as the presence of antigen, inflammation, and nutrients to regulate T cell growth, metabolism, and function. The tuberous sclerosis 1 (TSC1)/TSC2 complex negatively regulates the activity of an mTOR-containing multiprotein complex called mTOR complex 1. Recent studies have revealed an essential cell-intrinsic role for TSC1 in T cell survival, quiescence, and mitochondrial homeostasis. Given the emerging role of mTOR activity in the regulation of the quantity and quality of CD8 T cell responses, in this study, we examine the role of its suppressor, TSC1, in the regulation of antigen-specific primary and memory CD8 T cell responses to bacterial infection. Using an established model system of transgenic CD8 cell adoptive transfer and challenge with Listeria monocytogenes expressing a cognate antigen, we found that TSC1 deficiency impairs antigen-specific CD8 T cell responses, resulting in weak expansion, exaggerated contraction, and poor memory generation. Poor expansion of TSC1-deficient cells was associated with defects in survival and proliferation in vivo, while enhanced contraction was correlated with an increased ratio of short-lived effectors to memory precursors in the effector cell population. This perturbation of effector-memory differentiation was concomitant with decreased expression of eomesodermin among activated TSC1 knockout cells. Upon competitive adoptive transfer with wild-type counterparts and antigen rechallenge, TSC1-deficient memory cells showed moderate defects in expansion but not cytokine production. Taken together, these findings provide direct evidence of a CD8 T cell-intrinsic role for TSC1 in the regulation of antigen-specific primary and memory responses. C D8 T cells play a critical role in clearing several types of microbial infections by mounting robust cytotoxic T lymphocyte (CTL) responses that kill infected cells. A typical CTL response is characterized by the presence of an effector phase, a contraction phase, and a memory maintenance phase (1, 2). During the expansion phase, naive CD8 cells are activated by antigenpresenting cells (APCs) in secondary lymphoid organs and undergo rapid clonal expansion. At the peak of the response, the population of CD8 effectors is heterogeneous and consists of a majority of short-lived effector cells (SLECs) and a small pool of memory-precursor effector cells (MPECs) (3, 4). Following antigen clearance, 90 to 95% of the CD8 effectors undergo apoptosis during the contraction phase (5, 6). At this stage, MPECs are thought to preferentially contribute to the formation of a small but stable population of long-lived memory cells. These memory cells are capable of self-renewal in response to cytokines such as interleukin-15 (IL-15) and respond more rapidly than naive cells to antigen reexposure (7). A number of studies have correlated the kinetics of CD8 responses with external factors like the duration of antigen availability and ...