The replacement of main cap domain to improve the activity of a ZEN lactone hydrolase with broad substrate spectrum

Identification and Modification of Enzymatic Substrate Specificity through Residue Alteration in the Cap Domain: A Thermostable Zearalenone Lactonase

“…The V max (maximum velocity) and k cat (catalytic constant) were determined as 125.9 μM/min and 46.8 min –1 , respectively, resulting in a calculated k cat / K m (catalytic efficiency) of 0.203 μM –1 s –1 . This catalytic efficiency of ZENM was notably higher than that of ZHD11B . ZENM demonstrated excellent substrate affinity, displaying remarkably high efficiency in degrading ZEN (100% degradation of ZEN in 3 min).…”

“…The specific activities of ZHD11B toward ZEN, α-ZOL, β-ZOL, and α-ZAL were reported as 16.4, 11.9, 2.9, and 17.2 U/mg, respectively . On the other hand, ZHD101 showed a degradation efficiency of only 45% toward α-ZOL compared to its efficiency for ZEN degradation .…”

“…spinifera, F. monophora, and Neurospora crassa . It is worth noting that most ZEN lactonases have been identified as dimers, exemplified by CbZHD and ZHD101 from Cladophialophora bantiana and C.…”

“…Apart from microorganisms, enzymes have also proven to be efficient and eco-friendly biocatalysts for ZEN decontamination. , Lactonases play a crucial role in the feed industry for ZEN decontamination. , One such enzyme, lactonase ZHD101 from Clonostachys rosea IFO 7063, has shown remarkable capability in degrading ZEN . Additionally, lactonases from Fonsecaea monophora, Exophiala spinifera, and Rhinocladiella mackenziei have demonstrated the ability to degrade ZEN. However, the optimal temperature for these ZEN-degrading enzymes is below 50 °C, limiting their application in the feed industry.…”

Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil

“…Zearalenone degradation by ZHD is a hydrolysis reaction, and the reaction substrate is ZEN and water [ 25 ]. However, since the concentration of water changes little during the reaction process, it can be treated as a constant, so the reaction can be regarded as a single substrate enzyme catalyzed reaction.…”