The Repetitive DNA Content of Eukaryotic Genomes

López-Flores, Inmaculada; Garrido-Ramos, Manuel A.

doi:10.1159/000337118

Cited by 208 publications

(163 citation statements)

References 104 publications

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“…It is known that highly repetitive DNAs, especially the satDNAs, are subject to the action of several molecular mechanisms causing variation and allowing rapid evolution, generating speciesspecific sequences [Dover, 1986;Charlesworth et al, 1994;López-Flores and Garrido-Ramos, 2012]. These molecular mechanisms could have less impact in the Mahanarva genomes, as evidenced by the conservation detected here, although we do not know the type of sequences conserved because the Cot-DNA is anonymous.…”

Section: Discussionmentioning

confidence: 77%

Karyotypes and Repetitive DNA Evolution in Six Species of the Genus Mahanarva (Auchenorrhyncha: Cercopidae)

Anjos

Rocha

Paladini

et al. 2016

Cytogenet Genome Res

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Insects of the Cercopidae family are widely distributed and comprise 59 genera and 431 species in the New World. They are xylemophagous, causing losses in agricultural and pasture grasses, and are considered as emerging pests. Chromosomally, these insects have been studied by standard techniques, revealing variable diploid numbers and primarily X0 sex chromosome systems (males). We performed chromosome studies in 6 Mahanarva (Cercopidae) species using standard and differential chromosome staining as well as mapping of repetitive DNAs. Moreover, the relationship between the repetitive DNAs was analyzed at the interspecific level. A diploid chromosome number of 2n = 19,X0 was documented, with chromosomes gradually decreasing in size. Neutral or GC-rich regions were detected which varied depending on the species. Fluorescence in situ hybridization with a (TTAGG)_n telomeric motif probe revealed terminal signals, matching those of the Cot DNAs obtained from each species, that were also restricted to the terminal regions of all chromosomes. Dot blot analysis with the Cot fraction from M. quadripunctata showed that at least part of the repetitive genome is shared among the 6 species. Our data highlight the conservation of chromosomal features and organization of repetitive DNAs in the genus Mahanarva, suggesting a low differentiation for chromosomes and repetitive DNAs in most of the 6 species studied.

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Section: Discussionmentioning

confidence: 77%

Karyotypes and Repetitive DNA Evolution in Six Species of the Genus Mahanarva (Auchenorrhyncha: Cercopidae)

Anjos

Rocha

Paladini

et al. 2016

Cytogenet Genome Res

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“…We analyzed cold regions with regard to several genomic features that have been associated with suppressed crossing over in other regions (such as centromeres): low C+G content, low gene content, and enrichment for complex duplications and repeated sequences (López-Flores and Garrido-Ramos 2012). The results are presented in File S4.…”

Section: Resultsmentioning

confidence: 99%

High-Resolution Sex-Specific Linkage Maps of the Mouse Reveal Polarized Distribution of Crossovers in Male Germline

et al. 2014

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Since the publication of the first comprehensive linkage map for the laboratory mouse, the architecture of recombination as a basic biological process has become amenable to investigation in mammalian model organisms. Here we take advantage of high-density genotyping and the unique pedigree structure of the incipient Collaborative Cross to investigate the roles of sex and genetic background in mammalian recombination. Our results confirm the observation that map length is longer when measured through female meiosis than through male meiosis, but we find that this difference is modified by genotype at loci on both the X chromosome and the autosomes. In addition, we report a striking concentration of crossovers in the distal ends of autosomes in male meiosis that is absent in female meiosis. The presence of this pattern in both single- and double-recombinant chromosomes, combined with the absence of a corresponding asymmetry in the distribution of double-strand breaks, indicates a regulated sequence of events specific to male meiosis that is anchored by chromosome ends. This pattern is consistent with the timing of chromosome pairing and evolutionary constraints on male recombination. Finally, we identify large regions of reduced crossover frequency that together encompass 5% of the genome. Many of these “cold regions” are enriched for segmental duplications, suggesting an inverse local correlation between recombination rate and mutation rate for large copy number variants.

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“…Repetitive sequences are important components for genomic differentiation and evolutionary processes [Grewal and Jia, 2007;Cioffi and Bertollo, 2012;López-Flores and Garrido-Ramos, 2012;Biscotti et al, 2015;Costa et al, 2015]. Low heterochromatic content and homogeneous repetitive sequences have been associated with slow dynamics of karyotype repatterning imparting adverse conditions for high levels of genomic reorganization in some Percomorpha groups [Molina, 2007;Motta-Neto et al, 2011a, b, 2012Molina et al, 2013].…”

Section: Discussionmentioning

confidence: 99%

Paracentric Inversions Differentiate the Conservative Karyotypes in Two Centropomus Species (Teleostei: Centropomidae)

Borges

Cioffi

Bertollo

et al. 2019

Cytogenet Genome Res

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Centropomus is the sole genus of the Centropomidae family (Teleostei), comprising 12 species widely distributed throughout the Western Atlantic and Eastern Pacific, with 6 of them occurring in the Western Atlantic in extensive sympatry. Their life history and phylogenetic relationships are well characterized; however, aspects of chromosomal evolution are still unknown. Here, cytogenetic analyses of 2 Centropomus species of great economic value (C. undecimalis and C. mexicanus) were performed using conventional (Giemsa, Ag-NOR, and fluorochrome staining, C- and replication banding) and molecular (chromosomal mapping of 18S and 5S rDNA, H2A-H2B and H3 hisDNA, and (TTAGGG)_n repeats) approaches. The karyotypes of both species were composed of 48 solely acrocentric chromosomes (2n = 48; FN = 48), but the single ribosomal site was located in varying positions in the long arms of the second largest chromosome pair. Replication bands were generally similar, although conspicuous differences were observed in some chromosome regions. In both species, the histone H3 genes were located on 3 apparently homeologous chromosome pairs, but the exact position of these clusters differed slightly. Interspecific hisDNA and rDNA site displacements can indicate the occurrence of multiple paracentric inversions during the evolutionary diversification of the Centropomus genomes. Although the karyotypes remained similar in both species, our data demonstrate an unsuspected microstructural reorganization between them, driven most likely by a series of paracentric inversions.

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The Repetitive DNA Content of Eukaryotic Genomes

Cited by 208 publications

References 104 publications

Karyotypes and Repetitive DNA Evolution in Six Species of the Genus <b><i>Mahanarva </i></b>(Auchenorrhyncha: Cercopidae)

Karyotypes and Repetitive DNA Evolution in Six Species of the Genus <b><i>Mahanarva </i></b>(Auchenorrhyncha: Cercopidae)

High-Resolution Sex-Specific Linkage Maps of the Mouse Reveal Polarized Distribution of Crossovers in Male Germline

Paracentric Inversions Differentiate the Conservative Karyotypes in Two <b><i>Centropomus</i></b> Species (Teleostei: Centropomidae)

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