The Remedial Potential of Lycopene in Pancreatitis through Regulation of Autophagy

Choi, Suyun; Kim, Hye Young

doi:10.3390/ijms21165775

Cited by 18 publications

(17 citation statements)

References 112 publications

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“…Subsequently, the results suggested that the application of Angelicaoneu powders significantly reduced the inflammatory response, while the cell survival rate decreased and the apoptosis rate increased. The low expression of P62 and high expression of LC3-II confirmed that Angelicaoneu powders promoted autophagy to regulate the inflammatory response, while the increased AMPK and SIRT1 indicated that they were related to autophagy (24). Lycopene can improve pancreatitis by preventing oxidative stress-induced autophagy injury and/or directly activating autophagy in pancreatic acinocytes (25).…”

Section: Discussionmentioning

confidence: 90%

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Sun

Nie

2021

Cell Mol Biol (Noisy-le-grand)

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This research was carried out to investigate the effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate systemic inflammatory response in acute pancreatitis. In this study, the rat pancreatic acini AR42J cells were chosen as the research object, the application of hyla induced pancreatic acinar cells made model for acute pancreatitis, application of different concentrations of angelica peony spread effect on building cells, thus divided into control group, built in the module, the low concentration group, concentration and high concentration groups, determined by MTT method was applied to explore the above categories in cell proliferation, cell apoptosis was measured by flow cytometry, the expression of inflammatory factors in cell supernatant was determined by enzyme-linked immunoassay, and the expression of autophagy marker proteins LC3- ? and P62 was determined by Western-Bolt method. In order to explore the relationship between AMPK and SIRT1, immunoco-precipitation method was used to determine the interaction between AMPK and SIRT1, and dual luciferase experiment was used to explore the effect of AMPK on SIRT1. The AICAR group, BLM-275 group and negative control group were established. To explore the effect of SIRT1 on AMPK, we established SRT 1720 group, EX-527 group and control group. Direct binding between AMPK and SIRT1 should be determined by chromatin co-precipitation assay. In order to further explore the effect of AMPK/SIRT1 positive feedback loop on the systemic inflammatory response of acute pancreatitis, this study selected the medium-concentration Danggui Shaoyajiao SAN group as the control group (group C), and applied AMPK inhibitor BLM-275 and SIRT1 inhibitor EX 527 to the effect of medium-concentration Danggui Shaoyajiao SAN cells, respectively. The expression of autophagy marker proteins LC3- ? and P62 in groups A and B were determined by the Western-Bolt method. Results showed that compared with the control group, the cell survival rate, the expression of AMPK, SIRT1 and LC3-II in the model group were decreased, and the apoptosis rate of iNOS, IL-2, TNF-?, P62 and apoptosis were increased in the model group (P<0.05). the levels of iNOS, IL-2, TNF-?, P62 and cell survival rate in low, medium and high concentration groups decreased gradually, while the expressions of AMPK, SIRT1, LC3-II and cell apoptosis rate increased (P<0.05). The levels of iNOS, IL-2 and TNF-? in the three groups were gradually decreased with the increase of the concentration (P<0.05). Immunoprecipitation showed that AMPK and SIRT1 could bind to each other in cells. The double luciferase experiment indicated that the reporter gene containing the SIRT1 binding site was constructed. The luciferase activity was increased in THE AICAR group and decreased in the BLM-275 group (P<0.05). The reporter gene containing the AMPK promoter binding site was constructed. The luciferase activity in SRT1720 group was increased, while that in EX-527 group was decreased. SIRT1 could directly bind to the AMPK promoter. SIRT1 and LC3- ? protein expressions in group A were down-regulated, and P62 protein was increased (P<0.05). The protein expressions of AMPK and LC3- ? in group B were down-regulated, and the protein expression of P62 was increased (P<0.05). It concluded that AMPK can directly bind to activate SIRT1 expression, and SIRT1 expression can also activate AMPK, forming a positive feedback loop between the two. Therefore, Angelic Shaoyaodong decoction can mediate AMPK/SIRT1 positive feedback pathway to promote autophagy and regulate systemic inflammatory response in acute pancreatitis.

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Section: Discussionmentioning

confidence: 90%

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Sun

Nie

2021

Cell Mol Biol (Noisy-le-grand)

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“…Surprisingly, lycopene is the most prevalent carotenoid in the human diet [ 63 ]. Choi and Kim [ 64 ] recently published a review on the relevance of lycopene in pancreatitis, in which they concluded that lycopene, due to its antioxidative properties, could attenuate the severity of pancreatitis by preventing excess ROS-induced autophagy. Their conclusions were based on several papers, in which, for example, lycopene was reported to attenuate cadmium-induced activation of autophagy in murine hippocampal cells [ 52 ], rescue endothelial progenitor cells of type 2 diabetes mellitus (T2DM) rats from excess autophagy [ 53 ], and alleviate gentamicin-mediated cell death, apoptosis, and autophagy [ 54 ].…”

Section: Modulation Of Autophagy By Nutraceuticals: Support For the T...mentioning

confidence: 99%

“…Cho et al [ 68 ], in their review, listed carotenoids that are potentially relevant in ND therapy. Based on their paper, it seems that crocin [ 50 ], lutein [ 57 , 58 , 59 ], lycopene [ 52 , 54 , 64 ], astaxanthin [ 56 , 65 ], and fucoxanthin [ 61 ] could modulate autophagy under specific conditions, although the precise molecular mechanism of the autophagy-modulating activity of carotenoids remains unclear.…”

Section: Modulation Of Autophagy By Nutraceuticals: Support For the T...mentioning

confidence: 99%

Nutraceuticals as Modulators of Autophagy: Relevance in Parkinson’s Disease

Rakowski

Porębski

Grzelak

2022

IJMS

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Dietary supplements and nutraceuticals have entered the mainstream. Especially in the media, they are strongly advertised as safe and even recommended for certain diseases. Although they may support conventional therapy, sometimes these substances can have unexpected side effects. This review is particularly focused on the modulation of autophagy by selected vitamins and nutraceuticals, and their relevance in the treatment of neurodegenerative diseases, especially Parkinson’s disease (PD). Autophagy is crucial in PD; thus, the induction of autophagy may alleviate the course of the disease by reducing the so-called Lewy bodies. Hence, we believe that those substances could be used in prevention and support of conventional therapy of neurodegenerative diseases. This review will shed some light on their ability to modulate the autophagy.

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“…There was also decreased lipid peroxidation and oxidative stress overall. Numerous animal models have shown the benefits of antioxidant treatment on AP with a reduction in oxidative stress and inflammatory cytokines [46][47][48][49].…”

Section: Roles Of Antioxidant Therapy In Pancreatitis 41 Pharmacological Interventionmentioning

confidence: 99%

Antioxidant Therapy in Pancreatitis

2021

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Pancreatitis is pathologic inflammation of the pancreas characterized by acinar cell destruction and oxidative stress. Repeated pancreatic insults can result in the development of chronic pancreatitis, characterized by irreversible fibrosis of the pancreas and many secondary sequelae, ultimately leading to the loss of this important organ. We review acute pancreatitis, chronic pancreatitis, and pancreatitis-related complications. We take a close look at the pathophysiology with a focus on oxidative stress and how it contributes to the complications of the disease. We also take a deep dive into the evolution and current status of advanced therapies for management including dietary modification, antioxidant supplementation, and nuclear factor erythroid-2-related factor 2-Kelch-like ECH-associated protein 1(Nrf2-keap1) pathway activation. In addition, we discuss the surgeries aimed at managing pain and preventing further endocrine dysfunction, such as total pancreatectomy with islet auto-transplantation.

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The Remedial Potential of Lycopene in Pancreatitis through Regulation of Autophagy

Cited by 18 publications

References 112 publications

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Effect and mechanism of Angelic Shaoyaosan mediated AMPK/SIRT1 positive feedback loop to promote autophagy and regulate the systemic inflammatory response in acute pancreatitis

Nutraceuticals as Modulators of Autophagy: Relevance in Parkinson’s Disease

Antioxidant Therapy in Pancreatitis

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