The Remarkable Effect of Potassium Iodide in Eosin and Rose Bengal Photodynamic Action against Salmonella Typhimurium and Staphylococcus aureus

Santos, Adriele Rodrigues dos; Batista, Andréia Farias Pereira; Gomes, Ana T. P. C.; Neves, Maria G. P. M. S.; Faustino, Maria A. F.; Almeida, Adelaide; Hioka, Noboru; Mikcha, Jane M. G.

doi:10.3390/antibiotics8040211

Cited by 26 publications

(31 citation statements)

References 46 publications

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“…Indeed, we show that the presence of human plasma with its high HSA content can seriously interfere with aPDT using 1D9-700DX. However, this antioxidant activity can be completely overcome through the application of KI, a nontoxic salt that was previously shown to improve the aPDT efficacy in several models (42)(43)(44)(45). Importantly, KI has been approved by the US Food and Drug Administration for thyroid blocking in radiation emergencies, which underpins its therapeutic application potential.…”

Section: Discussionmentioning

confidence: 99%

Fighting Staphylococcus aureus infections with light and photoimmunoconjugates

Bispo¹,

Anaya-Sanchez²,

Suhani³

et al. 2020

JCI Insight

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Section: Discussionmentioning

confidence: 99%

Fighting Staphylococcus aureus infections with light and photoimmunoconjugates

Bispo¹,

Anaya-Sanchez²,

Suhani³

et al. 2020

JCI Insight

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“…The spectral emission of the LEDs system was obtained using a spectrofluorometer (Varian Cary Eclipse, San Diego, CA, USA). The absolute irradiance of the LEDs was evaluated with a Spectroradiometer USB2000 + RAD (OceanOptics, Winter Park, FL, USA) 19 .…”

Section: Methodsmentioning

confidence: 99%

Photoactivated cell-killing amino-based flavylium compounds

Oliveira

Araújo

Pereira

et al. 2021

Sci Rep

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Photodynamic therapy (PDT) is a well-established therapeutic for the treatment of different diseases. The growing interest of this technique required the development of new photosensitizers with better photo-features. This work reports the study of the potential of five nature-inspired amino-based flavylium compounds with different structural features as photosensitizers towards topical PDT. In terms of dark cytotoxicity the five pigments were tested towards confluent skin cells in both fibroblasts and keratinocytes. In the range of concentrations tested (6.3–100 μM), keratinocytes were more prone to growth inhibition and the IC50 values for 5OH4′NMe2, 7NEt2st4′NMe2 and 7NEt24′NH2 were determined to be 47.3 ± 0.3 μM; 91.0 ± 0.8 μM and 29.8 ± 0.8 μM, respectively. 7NEt24′NMe2, 7NEt2st4′NMe2 and 7NEt24′NH2 showed significant fluorescence quantum yields (from 3.40 to 20.20%) and production of singlet oxygen (1O2). These latter chromophores presented IC50 values of growth inhibition of keratinocytes between 0.9 and 1.5 µM, after 10 min of photoactivation with white light. This cellular damage in keratinocyte cells upon white light activation was accompanied with the production of reactive oxygen species (ROS). It was also found that the compounds can induce damage by either type I (ROS production) or type II (singlet oxygen) PDT mechanism, although a higher cell survival was observed in the presence of 1O2 quenchers. Overall, a structure–activity relationship could be established, ranking the most important functional groups for the photoactivation efficiency as follows: C7-diethylamino > C4′-dimethylamino > C2-styryl.

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“…To evaluate the potential of aPDT with NMB and MB in the photo-inactivation of Psa and to select the best photo-inactivation conditions to be used in the pollen disinfection, in vitro assays were performed. The first experiments in vitro were performed in phosphate-buffered saline (PBS), using NMB at different concentrations (1.0, 2.5, and 5.0 μM), without and with KI addition (an aPDT potentiator), selected according to our previous studies [ 26 , 27 ] at a concentration of 100 mM. In a second step, the best in vitro aPDT conditions established for NMB were extended to MB (concentration 5.0 μM) and the assays were performed with and without the addition of 100 mM of KI.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy in the Control of Pseudomonas syringae pv. actinidiae Transmission by Kiwifruit Pollen

et al. 2020

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Pseudomonas syringae pv. actinidiae (Psa) is a phytopathogen responsible for bacterial canker in kiwifruit plants and can be disseminated through pollen. This study aimed to evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) in the inactivation of Psa on kiwifruit pollen using New Methylene Blue (NMB) and Methylene Blue (MB) in the presence/absence of potassium iodide (KI). Pollen germination assays were also performed to evaluate if it was affected by aPDT. Higher reduction of Psa was achieved using NMB (5.0 μM) combined with KI (100 mM) in vitro (ca. 8 log CFU mL−1 after 90 min of irradiation), while NMB alone promoted a lower reduction (3.7 log CFU mL−1). The most efficient NMB concentration with KI was used to study the photodynamic efficiency of MB (5.0 μM). MB with KI photo-inactivated Psa more efficiently than NMB, causing the same bacterial reduction (ca. 8 log CFU mL−1) in half the irradiation time (45 min). Therefore, MB was selected for the subsequent ex vivo aPDT assays in pollen. Almost all the Psa cells added artificially to the pollen (3.2 log CFU mL−1) were photo-inactivated (3.1 log CFU mL−1), whereas aPDT had a low effect on pollen natural microorganisms. When KI was added, a significant increase in aPDT effectiveness was observed (4.5 log CFU mL−1). No negative effects were observed in the pollen germination after aPDT. The results show aPDT is an effective and safe method to Psa inactivation on kiwifruit pollen, and MB use is a promising alternative in the control of Psa transmission.

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The Remarkable Effect of Potassium Iodide in Eosin and Rose Bengal Photodynamic Action against Salmonella Typhimurium and Staphylococcus aureus

Cited by 26 publications

References 46 publications

Fighting Staphylococcus aureus infections with light and photoimmunoconjugates

Fighting Staphylococcus aureus infections with light and photoimmunoconjugates

Photoactivated cell-killing amino-based flavylium compounds

Antimicrobial Photodynamic Therapy in the Control of Pseudomonas syringae pv. actinidiae Transmission by Kiwifruit Pollen

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