The release of prostaglandins and progestin into the utero‐ovarian venous blood of guinea‐pigs during the oestrous cycle and following oestrogen treatment

Blatchley, F. R.; Donovan, B. T.; Horton, E. W.; Poyser, N.L.

doi:10.1113/jphysiol.1972.sp009835

Cited by 136 publications

(78 citation statements)

References 23 publications

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“…This increase in oestradiol output from the ovary precedes an increase in PGF2. output from the uterus by 24 h (Blatchley et al, 1972;Earthy et al, 1975;Antonini et al, 1976). Oestradiol acting on a progesterone-primed uterus appears to be the physiological stimulus for increased PGF2.…”

Section: Discussionmentioning

confidence: 99%

The effect of caffeine on prostaglandin output from the guinea‐pig uterus

Naderali

Poyser

1994

British J Pharmacology

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1 Caffeine increased the outputs of prostaglandin F2,. (PGF2.a), PGE2 and 6-keto-PGFI, from the guinea-pig uterus on days 7 and 15 of the oestrous cycle. The effect on PGE2 output depended on the age of the animals and was absent in younger guinea-pigs (<4 months). Theophylline also stimulated the outputs of PGF2,. and 6-keto-PGFI6, but not the output of PGE2, from the day 7 guinea-pig uterus. 2 The stimulatory effects of caffeine on the outputs of PGFu, PGE2 and 6-keto-PGFI. from the guinea-pig uterus were not prevented by lack of extracellular calcium, ryanodine or ruthenium red (both inhibitors of calcium release via the ryanodine receptor), although the increase in PGFu. output tended to be slower when extracellular calcium was absent. Also, ryanodine flattened and broadened the peak of increased PGFu. release.3 The calmodulin antagonists, W-7 and trifluoperazine, had no inhibitory effect on the caffeinestimulated increases in uterine prostaglandin output. In fact, W-7 (but not trifluoperazine) greatly potentiated the action of caffeine on uterine PGF2, output, but had little or no potentiating effect on the action of caffeine on uterine PGE2 and 6-keto-PGFIc outputs.4 TMB-8, an intracellular calcium antagonist, inhibited the increase in PGF2,, output produced by caffeine without preventing the increases in outputs of PGE2 and 6-keto-PGF1I. 5 These studies suggest that caffeine stimulates uterine PGF2, synthesis and release by a mechanism dependent upon intracellular calcium, but this mechanism is not mediated by activation of any of the three well-characterized ryanodine receptors or by calmodulin. Furthermore, the increases in the synthesis and release of PGE2 and 6-keto-PGFI. in the guinea-pig uterus induced by caffeine appear to involve mechanism(s) different from that which stimulates PGF2. production.

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Section: Discussionmentioning

confidence: 99%

The effect of caffeine on prostaglandin output from the guinea‐pig uterus

Naderali

Poyser

1994

British J Pharmacology

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“…However, the measurement of PGF-2\g=a\levels in the utero-ovarian venous plasma of cyclic guinea-pigs (Blatchley, Donovan, Horton & Poyser, 1972) revealed that, even though the concentration was low, PGF-2\g=a\was present throughout the oestrous cycle and that there was asynchrony in luteal regression (Days 12\p=n-\16: Rowlands, 1956) and the peak of PGF-2\g=a\secretion (Day 15: Blatchley et al, 1972). During experiments to study the effects ofPGF-2\g=a\on the different tissue components of the ovary, it was found that the CL responded to the luteolytic action of PGF-2\g=a\only at certain times.…”

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confidence: 99%

The effect of continuous treatment with prostaglandin F-2 on oestrous cycle length and corpus luteum regression in hysterectomized guinea-pigs

Tso¹,

Tam²

1977

Reproduction

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It has been established that prostaglandin (PG) F-2\g=a\induces the involution of corpora lutea (CL) in hysterectomized guinea-pigs (Blatchley & Donovan, 1969) and that the guinea-pig uterus is probably the major source of this luteolytic factor (Poyser, 1972). However, the measurement of PGF-2\g=a\levels in the utero-ovarian venous plasma of cyclic guinea-pigs (Blatchley, Donovan, Horton & Poyser, 1972) revealed that, even though the concentration was low, PGF-2\g=a\was present throughout the oestrous cycle and that there was asynchrony in luteal regression (Days 12\p=n-\16: Rowlands, 1956) and the peak of PGF-2\g=a\secretion (Day 15: Blatchley et al., 1972). During experiments to study the effects ofPGF-2\g=a\on the different tissue components of the ovary, it was found that the CL responded to the luteolytic action of PGF-2\g=a\only at certain times.Guinea-pigs were hysterectomized before puberty (24-32 days old) and then injected i.p. twice every day with 0-125 mg PGF-2a (equivalent to 0-168 mg THAM salt, Upjohn Co.) in 0-5 ml 0-9% NaCl, i.e. 0-057-0-100 mg PGF-2a/100 g body weight/day (Group A). Other guinea-pigs of the same age were (i) hysterectomized and injected twice daily with 0-5 ml NaCl (Group B), (ii) subjected to sham-operation, i.e. sectioning of the utero-vaginal junction but leaving the uterine horns in situ, and similarly treated with NaCl (Group C), and (iii) given no treatment (Group D). Most animals were killed in the second oestrous cycle, but 3 Group-and 3 Group-D animals were maintained until the early stages of the fourth cycle. At autopsy the ovaries were fixed and serially sectioned for histological observation. The CL volume was calculated from the mean of 3 diameters set at right angles to one another, measured with an ocular micrometer from the ovarian sections. Oestrous cycle length was calculated by daily examination of the vaginal closure membrane and the day after the first day the vaginal membrane was fully perforate was taken to be Day 1 of the cycle.The first vaginal opening was observed in animals of Groups C and D when they were 36-2 ± 0-8 (N = 23) days old (mean ± S.E.M., the total of 11 animals in Group C and 12 animals in Group D), but the first vaginal opening occurred at 45-2 ± 2-9 (N = 14) days of age in the PGF-2a-treated animals in Group A. It is not clear whether this delay was due to the combined effect of hysterectomy and PGF-2a treatment or just the shock of hysterectomy as the amount of blood loss in hysterectomy was always greater than in the sham-operation. However, once the first oestrus had occurred the cycle lengths in Group A were, for the first to third cycle, 14-9 ± 0-6 (number of cycles observed = 12), 15-0 ±1-2 (3) and 16-3 ± 0-9 (3) days, respectively; and these durations were not significantly differ¬ ent (Student's / test) from those of the control animals in Group D (15-4 ± 0-6 (9), 16-3 ± 0-3 (3) and 15-7 ± 0-3 (3) days, respectively). There was also no significant difference between the duration of the first cycle in Groups C and D. Continual t...

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“…Bland & Donovan (1968 have shown that injections of oestradiol benzoate on days 3-11 of the oestrous cycle, or a single injection on day 3 led to regression of the corpora lutea of guinea-pigs, an effect abolished by hysterectomy. Oestradiol benzoate injections early in the cycle also gave rise to increased prostaglandin F2a levels in utero-ovarian venous blood (Blatchley, Donovan, Poyser, Horton, Thompson & Los, 1971;Blatchley et al, 1972) though not in hysterectomized animals, indicating that the prostaglandin F2.…”

Section: Discussionmentioning

confidence: 99%

“…In the guinea-pig, the increase in oestradiol secretion from the ovary towards the end of the oestrous cycle (Joshi et al, 1973) may be the stimulus for increased prostaglandin F1 production and release from the uterus at this time (Poyser, 1972;Blatchley et al, 1972). Bland & Donovan (1968 have shown that injections of oestradiol benzoate on days 3-11 of the oestrous cycle, or a single injection on day 3 led to regression of the corpora lutea of guinea-pigs, an effect abolished by hysterectomy.…”

Section: Discussionmentioning

confidence: 99%

“…To these samples was added 1 pg deuterated (d4 ) prostaglandin F2ac before extraction. After extraction the prostaglandins were prepared as the methyl ester-trimethylsilyl ether (Me-TMS), (Thompson, Los & Horton, 1970;Blatchley, et al, 1972) IV-oestradiol-173 (100,ug/ml) and progesterone (500 Ag/ml). The procedure was designed so that one portion of each uterus was included in each group.…”

Section: Introductionmentioning

confidence: 99%

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EFFECTS OF OESTRADIOL AND PROGESTERONE ON THE in vitro PRODUCTION OF PROSTAGLANDIN F_2α BY THE GUINEA‐PIG UTERUS

Naylor

Poyser

1975

British J Pharmacology

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I The effects of oestradiol and progesterone on the production of prostaglandin F2. by the guinea-pig uterus in vitro have been studied. 2 Ovariectomized guinea-pigs were treated with oestradiol benzoate, progesterone, or a combination of these hormones. The uteri were homogenized and incubated and the prostaglandin F2c, produced was measured by radioimmunoassay or combined gas chromatography-mass spectrometry (g.c.-m.s.). Oestradiol treatment or progesterone followed by oestradiol caused a significant increase in prostaglandin F2csynthesis compared with control values, whereas progesterone treatment caused no significant increase. 3 The uteri from guinea-pigs on day 7 of the oestrous cycle were split into four parts, and each part homogenized and incubated either alone or in the presence of oestradiol-17j, progesterone, or a combination of these hormones. Prostaglandin F2,,was measured by g.c.-m.s. Oestradiol was found to increase prostaglandin F2c, synthesis by the uterus whereas progesterone had no effect. However, progesterone did inhibit the response to oestradiol. 4 Oestradiol treatment of the guinea-pig uterus causes an increase in capacity to produce prostaglandin F2Oin vitro. The role of progesterone is difficult to interpret.

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The release of prostaglandins and progestin into the utero‐ovarian venous blood of guinea‐pigs during the oestrous cycle and following oestrogen treatment

Cited by 136 publications

References 23 publications

The effect of caffeine on prostaglandin output from the guinea‐pig uterus

The effect of caffeine on prostaglandin output from the guinea‐pig uterus

The effect of continuous treatment with prostaglandin F-2 on oestrous cycle length and corpus luteum regression in hysterectomized guinea-pigs

EFFECTS OF OESTRADIOL AND PROGESTERONE ON THE in vitro PRODUCTION OF PROSTAGLANDIN F_2α BY THE GUINEA‐PIG UTERUS

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The release of prostaglandins and progestin into the utero‐ovarian venous blood of guinea‐pigs during the oestrous cycle and following oestrogen treatment

Cited by 136 publications

References 23 publications

The effect of caffeine on prostaglandin output from the guinea‐pig uterus

The effect of caffeine on prostaglandin output from the guinea‐pig uterus

The effect of continuous treatment with prostaglandin F-2 on oestrous cycle length and corpus luteum regression in hysterectomized guinea-pigs

EFFECTS OF OESTRADIOL AND PROGESTERONE ON THE in vitro PRODUCTION OF PROSTAGLANDIN F2α BY THE GUINEA‐PIG UTERUS

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EFFECTS OF OESTRADIOL AND PROGESTERONE ON THE in vitro PRODUCTION OF PROSTAGLANDIN F_2α BY THE GUINEA‐PIG UTERUS