The relative contribution of antibody production and CD8⁺ T cell function to immune control of Trypanosoma cruzi

Abstract

The life cycle of the protozoan parasite Trypanosoma cruzi in mammalian hosts includes both non-dividing trypomastigote forms which circulate in the blood and replicating intracellular amastigotes which reside within the cytoplasm of a variety of host cells. In this study we have used mice with induced mutations in genes responsible for either antibody production or cytolytic T lymphocyte (CTL) function to examine the relative contributions of these effector mechanisms to control of T. cruzi. Mice deficient in the production of antibodies exhibited a delay in the rise in acute phase parasitaemia and an extended time to death relative to mice lacking CD8+ T cells. Nevertheless, B cell deficient mice eventually succumbed to the infection. Prior infection with an avirulent strain of T. cruzi failed to protect either CD8+ T cell-deficient mice or B cell deficient mice from challenge infection with virulent parasites. In contrast, mice with disruptions in the genes controlling perforin- or granzyme B-mediated cytolytic pathways had parasitaemia and mortality rates similar to wild-type mice and were protected from secondary infection by prior exposure to avirulent parasites. These results 1) confirm that antibody production, although secondary in importance to cellular responses, is nevertheless absolutely required and 2) perforin- or granzyme B-mediated lytic pathways are not required for control of T. cruzi infection.

Swimming against the current: genetic vaccination against Trypanosoma cruzi infection in mice

“…However, it is unclear whether lysis and DNA degradation of infected target cells by CD8 + T cells can be effective in restricting T. cruzi infection in vivo. Genetically deficient mice that do not make perforin or granzyme B are not more susceptible to infection than wild-type mice after infection with the Brazil strain of T. cruzi (Kumar & Tarleton 1998). However, other T. cruzi strains behave differently and perforin-deficient mice are highly susceptible to infection (Tzelepis et al 2006).…”

Immunopathology of Chagas disease

“…These cells were seen to express the Gramzyme A cytotoxic factor . It has been now a consensus that CD8 + lymphocytes are the main T-cell type responsible for immune activation in chronic chagasic myocarditis (Kumar & Tarleton 1998). These cells are activated, through Class I MHC molecules, by macrophages containing remnants of T. cruzi.…”

Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF‐α secretion and reduce parasite replication in macrophages

“…Ab play a protective role in T. cruzi infection [1][2][3][4][5]7], but the mechanisms involved are not completely understood.…”