The Relationship of the Age of Rabbit Erythrocytes to the Effects of Inosine on their Osmotic Resistance12

Jaffé, Ernst R.; Vanderhoff, Grace A.; Lowy, Bertram A.; London, Irving M.

doi:10.1172/jci103717

Cited by 9 publications

(2 citation statements)

References 16 publications

(15 reference statements)

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“…Only reticulocyte-rich blood underwent delayed hemolysis ; osmotic fragility curves of blood samples containing less than 10% reticulocytes were not shifted measurably following incubation for 24 hours at 37"C, agreeing with the observations of Jaffe et al ( 7 ) . Only reticulocyte-rich blood underwent delayed hemolysis ; osmotic fragility curves of blood samples containing less than 10% reticulocytes were not shifted measurably following incubation for 24 hours at 37"C, agreeing with the observations of Jaffe et al ( 7 ) .…”

Section: Alkyl Polyamtnes I N Reticulocyte Lysissupporting

confidence: 90%

Inhibition of Reticulocyte Lysis by Alkyl Polyamines

Little¹

1962

Experimental Biology and Medicine

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phopyridine nucleotides have been studied in rat liver after fasting and during oxidation of ethanol. The changes in diphosphopyridine nucleotides have also been studied in baker's yeast during ethanol oxidation. By fasting alone a slight decrease of DPN/DPNH ratio mainly due to a fall in the concentration of DPN is noted. Total DPN + DPNH is also decreased slightly. n'hen the animals are metabolizing ethanol the DPN,'DPNH ratio decreases further, due to an increase of DPNH, and especially in the fasted animals a fairly marked increase of the total DPN + DPNH can be noted. Similar changes are observed in baker's yeast during ethanol oxidation. Triphosphopyridine nucleotide is mainly present in the liver in its reduced form, and the TJ'N/TIWH does not. change significantly during ethanol oxidation. Inhibition of Reticulocyte Lysis by AlkylPolyamines. (27375) J. R. LITTLE (Introduced by G. Brecher) Hematology Scrvice, c'lin. Path. Dept., Clinii.cal Cenlrr, .V.I.II., P.H.S., Hethesda, M d .The participation of the alkyl polyamines in many diverse biological and biochemical phenomena has made this class of organic compounds a source of increasing interest. Spermine, spermidine and related substances are widely distributed in animal tissues and microorganisms ; yet specific information about their function(s) is lacking. A variety of biological effects has been demonstrated in selected systems ( 1 ) including: 1 ) promotion of growth of microorganisms, 2 ) stabilization of bacteria, bacterial protoplasts, mitochondria, ribosomes, bacteriophages, and transforming principle, 3) binding to nucleic acids, heparin, and certain lipids, and 4) stabilization of enzyme activity.This report deals with the stabilizing effect of spermine and related polyamines on the reticulocyte undergoing spontaneous lysis.Methods. Blood samples, anticoagulated with dry dipotassium versenate, were obtained from human subjects and Sprague-Dawley rats. Reticulocyte-rich blood was obtained from female Sprague-Dawley rats weighing 150-200 g, previously given subcutaneous injections of phenylhydrazine hydrochloride( 2). Reticulocyte counts of up to SO: %, were obtained. Reticulocyte counts were made on all blood samples using the methods of Brecher (3,4).Dilute suspensions of red blood cells and reticulocytes were incubated in phosphate buffered saline following the osmotic fragility method of Dacie ( 5 ) . For each blood sample tested, a duplicate series of tubes was used containing 2 ml aliquots of serial dilutions of 0.150 hl NaCl in 0.01 M sodium phosphate buffer at pH 7.40. To one series of tubes 0.01 ml of an alkyl amine solution was added so that the final concentration of amine was 0.5 mM. A second series of tubes without alkyl amine served as a control. Whole blood (0.02 ml) was then delivered into each tube and mixed immediately by gentle inversion. Tubes were incubated at room temperature ( 23"-2SoC) for 30 minutes and then centrifuged at 2000 rpm for 5 minutes. The amount of hemolysis (referred to as immediate hemolysis) was determined b...

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Section: Alkyl Polyamtnes I N Reticulocyte Lysissupporting

confidence: 90%

Inhibition of Reticulocyte Lysis by Alkyl Polyamines

Little¹

1962

Experimental Biology and Medicine

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“…However, a relationship between the age of erythrocytes and their resistance to hypotonic lysis has been described (27). The absence of a distinct three phase cyclic change in normal erythrocytes at tonicities greater than 0.50 per cent "saline" might be the result of the oldest erythrocytes exhibiting only a single phase.…”

Section: Methodsmentioning

confidence: 99%

Studies on the Osmotic Fragility of Incubated Normal and Abnormal Erythrocytes*

Haut¹,

Tudhope²,

Cartwright³

et al. 1962

J. Clin. Invest.

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The osmotic fragility of erythrocytes is known to undergo alterations when whole blood is incubated under aseptic conditions for 24 hours. The osmotic fragility of spherocytes increases considerably ( 1 ). Normal erythrocytes undergo a slight increase in fragility (2) and by contrast the osmotic fragility of leptocytes and certain other erythrocytes (3) is decreased. The sequence of events associated with this divergent change and the factors which influence it are the subjects of this study.Under certain circumstances the normal erythrocyte may behave as an osmometer (4). Though not implying "rupture" of the erythrocyte (5), osmotic lysis occurs when the red cell volume increases to a critical value (6, 7). In in vitro systems, the degree of osmotic lysis which may occur is affected by pH, temperature, and tonicity (8) of the surrounding medium. If these variables are held constant, then alterations in the osmotic fragility of the incubated erythrocytes would reflect changes in their milieu interne which result from metabolic activity during incubation.Abnormality of shape or volume of erythrocytes or both is presumed to alter their osmotic fragility by virtue of altering the span between the initial volume and the critical hemolytic volume of the cell (6, 7, 9). However, abnormal cell geometry does not, by this means, account for the apparent paradoxical response of the osmotic fragility of leptocytes to incubation. If the initial direction of change in the osmotic fragility of incubated leptocytes were abnormal, an abnormality of erythrocyte metabolism would be suggested. If the initial change in osmotic fragility were normal but

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Die Biochemie des Erythrocyten, mit Ausnahme des Hämoglobinstoffwechsels

Büsch¹

1968

Blut Und Blutkrankheiten

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The Relationship of the Age of Rabbit Erythrocytes to the Effects of Inosine on their Osmotic Resistance12

Cited by 9 publications

References 16 publications

Inhibition of Reticulocyte Lysis by Alkyl Polyamines

Inhibition of Reticulocyte Lysis by Alkyl Polyamines

Studies on the Osmotic Fragility of Incubated Normal and Abnormal Erythrocytes*

Die Biochemie des Erythrocyten, mit Ausnahme des Hämoglobinstoffwechsels

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