The relationship between the serogroup antigen and lipopolysaccharide of<i>Legionella pneumophila</i>

Conlan, J. Wayne; Ashworth, L.A.E.

doi:10.1017/s0022172400062513

Cited by 38 publications

(14 citation statements)

References 28 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…E. coli or Salmonella spp. ), L. pneumophila lipopolysaccharide could not be isolated from the water phase but, instead, was identified in the phenol phase [21,23,25,27, 281 indicating a different physicochemical behaviour, i.e. an increased hydrophobicity.…”

Section: Discussionmentioning

confidence: 99%

The structure of the O‐specific chain of Legionella pneumophila serogroup 1 lipopolysaccharide

Knirel

Rietschel

Marre

et al. 1994

European Journal of Biochemistry

119

102

View full text Add to dashboard Cite

The 0-polysaccharide chain of Legionella pneumophila Philadelphia strain 1 (serogroup 1) lipopolysaccharide was investigated by means of 'H-and I3C-NMR spectroscopy, and chemical analysis. It was found to consist of an a-(2+4) interlinked homopolymer of a 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-nonulosonic acid possessing most likely the D-glycero-L-galacto configuration, representing the first example of an acidic homopolymer of a higher sugar of this class. The ladder-like banding pattern exhibiting small distances between individual bands in the SDS/ PAGE is compatible with a monosaccharide repeating unit.Legionella pneumophila constitutes the etiologic agent of legionellosis and may cause severe respiratory infection in susceptible individuals, including man [l]. Bacteria of the genus Legionella are facultative intracellular pathogens which multiply within the phagosome of monocytes [2]. Moreover, legionellae are not killed efficiently after being phagocytized by polymorphonuclear leukocytes. Therefore, the pathogenicity of Legionella spp. lasgely depends on the outcome of interaction between the bacterium and the phagocytes it encounters in the host. However, little is known today about the biochemical mechanism involved in their interaction, i.e. the factors mediating bacterial virulence. Several bacterial compounds have been implicated as pathogenicity factors including toxins, enzymes and cell-surface proteins [2]. A further possible candidate of mediating bacterial virulence is the outer membrane constituent lipopolysaccharide. Lipopolysaccharide is known to contribute to the pathogenicity of enterobacteria [3] and it is likely to be also important in Legionella infection. In particular, the 0-specific chain was shown to be of importance in virulence [3], and we therefore, attempted to elucidate the chemical structure of the 0-polysaccharide of the Legionella lipopolysaccharide.in the present study the structure of the 0-specific polysaccharide of L. pneumophila serogroup 1 lipopolysaccharide was elucidated. It will be shown that it constitutes a hitherto unknown homopolymer of 5-acetamidino-7-acetamido-8-0-acetyl-3,5,7,9-tetadeoxy-D-glycero-L-ga~acto-nonu~oson~c acid.Correspondence to U. Ziihringer, Forschungsinstitut Borstel, Institut fur Experimentelle Biologie und Medizin, Parkallee 22, D-23845 Borstel, GermanyAbbreviations. QuiN, quinovosamine, 2-amino-2,6-dideoxy-glucose ; Kdo, 3-deoxy-D-mnno-2-octulosonic acid; PSNa0,,, polysaccharide obtained after hydrolysis of the lipopolysaccharide with sodium acetate buffer; PS,,,,, OH, and PS,,,,.PS, , , , after alkaline hydrolysis in sodium hydroxide (NaOH), or triethylamine (Et,N); PS,,, and PS,,,,, polysaccharide obtained by hydrolysis with hydrochloric (HCI), or acetic (AcOH) acid.

show abstract

Section: Discussionmentioning

confidence: 99%

The structure of the O‐specific chain of Legionella pneumophila serogroup 1 lipopolysaccharide

Knirel

Rietschel

Marre

et al. 1994

European Journal of Biochemistry

119

102

View full text Add to dashboard Cite

show abstract

“…The serogroup specificity of L. pneumophila is defined by the structure of the outer-membrane LPS [14,15]. Recently, the structure of the 0-specific chain of LPS of L. pneumophila serogroup 1 strain Philadelphia 1 was elucidated [16].…”

Section: J H Helbig and Othersmentioning

confidence: 99%

Molecular characterization of a virulence-associated epitope on the lipopolysaccharide ofLegionella pneumophilaserogroup 1

et al. 1995

View full text Add to dashboard Cite

SUMMARYFor identification of lipopolysaccharide (LPS)-associated epitopes of Legionella pneumophila serogroup 1, LPS of strain Philadelphia 1 was investigated using monoclonal antibodies (MAbs). The 0-specific chain of LPS is a homopolymer of 5-acetamidino-7-acetamido-8-0-acetyl-3,5,7,9-tetradeoxy-D-glycero-L-galactononulosonic acid. At least four immunoaccessible epitopes were recognized by different MAbs on the intact LPS. After O-deacetylation of LPS, the reactivity of one of the MAbs (MAb 3/1) was lost, indicating thus that the corresponding epitope is associated with the 8-0-acetyl group. Since the reactivity pattern of the MAb 3/1 is identical with those of the MAb 2 which was considered as a virulence marker for serogroup 1, this epitope may be involved in mediating virulence in L. pneumophila. Four MAbs specific to strains of serogroup 1 other than the monoclonal subtype Philadelphia recognized epitopes on the O-deacetylated LPS of strain Philadelphia 1 and, therefore, the virulence-associated epitope blocks recognition of the immunodeterminants that are accessible on the intact LPS of the strains lacking this epitope.

show abstract

“…Chemical analysis of Legionella LPS was performed decades ago (Conlan and Ashworth, 1986), but the size and the chemical composition of the excreted polysaccharide are not yet known.…”

Section: Other Urinary Tests For Disseminated Mycosesmentioning

confidence: 99%

Urine as a Specimen to Diagnose Infections in Twenty-First Century: Focus on Analytical Accuracy

Tuuminen¹

2012

Front. Immun.

View full text Add to dashboard Cite

Urine as a clinical specimen to diagnose infections has been used since ancient times. Many rapid technologies to assist diagnosis of infections are currently in use. Alongside traditional enzyme immunoassays (EIA), new technologies have emerged. Molecular analysis of transrenal DNA to diagnose infections is also a rapidly growing field. The majority of EIAs utilize the detection of excreted sugar compounds of the outer microbial cell-wall shed into the bloodstream and excreted into the urine. This mini-review focuses on current knowledge on rapid urinary antigen detection tests to diagnose most common infections, and highlights their diagnostic utility. The past and the future of urinalysis are also briefly discussed. The analysis of the literature shows that some methods are not quantitative, and analytical sensitivity may remain suboptimal. In addition, the performance criteria and technical documentation of some commercial tests are insufficient. Clinical microbiologists and physicians should be alert to assay limitations.

show abstract

The relationship between the serogroup antigen and lipopolysaccharide ofLegionella pneumophila

Cited by 38 publications

References 28 publications

The structure of the O‐specific chain of Legionella pneumophila serogroup 1 lipopolysaccharide

The structure of the O‐specific chain of Legionella pneumophila serogroup 1 lipopolysaccharide

Molecular characterization of a virulence-associated epitope on the lipopolysaccharide ofLegionella pneumophilaserogroup 1

Urine as a Specimen to Diagnose Infections in Twenty-First Century: Focus on Analytical Accuracy

Contact Info

Product

Resources

About