The relationship between the age of bacillus subtilis spores and their resistance to ethylene oxide

Bomar, M.T.

doi:10.1007/bf02930775

Folia Microbiol

1962

DOI: 10.1007/bf02930775

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The relationship between the age of bacillus subtilis spores and their resistance to ethylene oxide

M.T. Bomar

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1973

1983

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“…Numerous other papers were published on the effects of ethylene oxide on bacteria and viruses previously known as resistant or pathogenic (3,5,31,39,42,44). It was soon found that the numerous bacterial preparations used had a decisive effect on the success of sterilization (6,9,10,11,35,38,41). In the following period, the main parameters of ethylene oxide fumigation were determined; without a knowledge of these, our present ethylene oxide sterilization would not be practicable (13, 14, 16, 19, 20, 22-25, 28, 29, 32, 36, 40).…”

mentioning

confidence: 99%

Sorption Processes in Gas Sterilization in the Medical Sector

Jordy¹,

Suhr²

1973

Applied Microbiology

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mentioning

confidence: 99%

Sorption Processes in Gas Sterilization in the Medical Sector

Jordy¹,

Suhr²

1973

Applied Microbiology

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Factors influencing the resistance of biological monitors to ethylene oxide

Dadd

McCormick

Daley

1983

Journal of Applied Bacteriology

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The resistance of bacterial spore monitors is markedly influenced by the environmental conditions existing during development of spores and, subsequently, in the preparation and evaluation of the monitor. Sporulation medium, suspending medium, pasteurization and storage conditions influence resistance of spores of Bacillus subtilis var. niger to ethylene oxide, but incubation temperature and age of sporulating culture appear to be unimportant. The conditions under which the spore suspension is dried on the supporting medium of the monitor exerts a major influence on resistance. Spores exposed to ethylene oxide are abnormally susceptible to damage by shaking with Ballotini, a method frequently used to recover spores from monitors. Nutritional conditions, pH and temperature of incubation influence the ability of survivors to form colonies on solidified media.

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Sorption Processes in Gas Sterilization in the Medical Sector

Jordy¹,

Suhr²

1973

Appl Microbiol

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Sorption of ethylene oxide during and after gaseous sterilization is influenced by numerous factors. It was found that ethylene oxide desorption not only depends on material to be fumigated but also to a considerable degree on the wrapping material. Although polyethylene, polyamide (nylon), polytetrafluoroethylene (Teflon), silicone, aluminum, and glass beads contained no quantities of ethylene oxide detectable by gas chromatography after 72 h of aeration, residual amounts were definitely determined, even after 76 h of aeration in polypropylene, polystyrene, polyvinylchloride, paper products, and compound products of various plastics and paper mixtures. Desorption was, in all cases, found to be better when a mixture of ethylene oxide and methyl formate was used instead of pure ethylene oxide.

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The relationship between the age of bacillus subtilis spores and their resistance to ethylene oxide

Cited by 3 publications

References 7 publications

Sorption Processes in Gas Sterilization in the Medical Sector

Sorption Processes in Gas Sterilization in the Medical Sector

Factors influencing the resistance of biological monitors to ethylene oxide

Sorption Processes in Gas Sterilization in the Medical Sector

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