The relationship between sperm DNA fragmentation, free radicals and antioxidant capacity with idiopathic repeated pregnancy loss

Kamkar, Narges; Ramezanali, Fariba; Sabbaghian, Marjan

doi:10.1016/j.repbio.2018.11.002

Cited by 51 publications

(57 citation statements)

References 25 publications

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“…34,47,48 Sperm nDNA fragmentation is often associated with decreased semen antioxidant capacity and has been reported to be a major contributor to spontaneous miscarriages. 49 MP showed modulatory effects on sperm parameters, which were comparable with metformin, while the combined therapy showed better improvements when compared to the monotherapy groups, thus suggestive of a likely complementary effect of both treatments. We hypothesize that the improvements following the different interventions may be attributed to Indian propolis were reported to counteract the effects of chemotherapeutic agents (doxorubicin and mitomycin C, respectively) on sperm count, motility, morphology and nDNA fragmentation, by decreasing testicular oxidative stress and improving steroidogenesis.…”

Section: Discussionmentioning

confidence: 83%

Malaysian propolis and metformin mitigate subfertility in streptozotocin‐induced diabetic male rats by targeting steroidogenesis, testicular lactate transport, spermatogenesis and mating behaviour

et al. 2019

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Background: Diabetes mellitus is one of the risk factors for male subfertility/infertility. Malaysian propolis is reported to decrease hyperglycaemia in diabetic state. Objectives:The present study investigated the protective effect of Malaysian propolis on diabetes-induced subfertility/infertility. Additionally, its combined beneficial effects with metformin were investigated. Materials and methods:Forty adult male Sprague Dawley rats were randomly assigned into five groups, namely normal control, diabetic control, diabetic + Malaysian propolis (300 mg/k.g. b.w.), diabetic + metformin (300 mg/kg b.w.) and diabetic + Malaysian propolis + metformin. Diabetes was induced using a single intraperitoneal injection of streptozotocin (60 mg/kg b.w.) and treatment lasted for 4 weeks. During the 4thweek, mating behavioural experiments were performed using sexually receptive female rats. Thereafter, fertility parameters were assessed in the female rats. Results: Malaysian propolis increased serum and intratesticular free testosterone levels, up-regulated the mRNA levels of AR and luteinizing hormone receptor, upregulated the mRNA and protein levels of StAR, CYP11A1, CYP17A1, 3β-HSD and 17β-HSD in the testes of diabetic rats. Furthermore, Malaysian propolis up-regulated testicular MCT2, MCT4 and lactate dehydrogenase type C mRNA levels, in addition to improving sperm parameters (count, motility, viability and normal morphology) and decreasing sperm nDNA fragmentation in diabetic rats. Malaysian propolis improved mating behaviour by increasing penile guanosine monophosphate levels. Malaysian propolis also improved fertility outcome as seen with decreases in pre-and postimplantation losses, increases in gravid uterine weight, litter size per dam and foetal weight. Malaysian propolis's effects were comparable to metformin. However, their combination yielded better results relative to the monotherapeutic interventions. Conclusion: Malaysian propolis improves fertility potential in diabetic state by targeting steroidogenesis, testicular lactate metabolism, spermatogenesis and mating

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Section: Discussionmentioning

confidence: 83%

Malaysian propolis and metformin mitigate subfertility in streptozotocin‐induced diabetic male rats by targeting steroidogenesis, testicular lactate transport, spermatogenesis and mating behaviour

et al. 2019

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“…The rates of abnormal sperm morphology and sperm DNA fragmentation have been reported to be significantly increased in patients with repeated abortions compared with those of fertile men without ART treatments (Esquerré‐Lamare et al, 2018; Kamkar, Ramezanali, & Sabbaghian, 2018; Zidi‐Jrah et al, 2016). A meta‐analysis review showed that the NSMR was decreased among men with unexplained repeated abortions compared with the control group (Cao et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Effects of the normal sperm morphology rate on the clinical and neonatal outcomes of conventional IVF cycles

Chen

Liu

et al. 2020

Andrologia

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The present study assessed the influences of the normal sperm morphology rate on the clinical and neonatal outcomes of conventional in vitro fertilisation cycles. This retrospective study analysed 427 and 2,728 cycles from the normal sperm morphology rate <4% and ≥4% group respectively. The clinical (total fertilisation failure, clinical pregnancy, implantation and abortion) and neonatal (sex, gestational age, preterm birth, birthweight, low birth weight, live births and birth defects of newborns) outcomes were compared. The rate of total fertilisation failure in the normal sperm morphology rate <4% group was significantly higher compared with that in the normal sperm morphology rate ≥4% group (2.8% versus 1.2%, p = .012). Total fertilisation failure was completely resolved by early rescue intracytoplasmic sperm injection. The clinical pregnancy, implantation and abortion rates were not significantly different between the two groups. Additionally, the sex, preterm birth, low birth weight, live births and birth defect rates, gestational age and birthweight of newborns were not significantly different between the two groups. Thus, normal sperm morphology rate <4% significantly increased the total fertilisation failure rate but did not affect the clinical or neonatal outcomes.

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“…Oxidative stress occurs in results of oxidative imbalance by an increase in reactive oxygen species (ROS) or by decrease/absence in antioxidant status (Gulum et al, ; Riaz et al, ). This process can lead to damages to polyunsaturated lipids present in sperm plasma membrane, as well as to the DNA, RNA and proteins (Atig, Kerkeni, Saad, & Ajina, ; Kamkar, Ramezanali, & Sabbaghian, ; Mello Filho et al, ; Sposito et al, ). This imbalance can also be observed in reproductive biotechnologies, (e.g., semen cryopreservation; Amidi, Pazhohan, Shabani, Khodarahmian, & Nekoonam, ; Panner Selvam et al, ), in vitro fertilisation and in protocols with an excessive manipulation of semen, as plasma separation through centrifugation (Donnelly et al, ) and also sperm separation (Raad et al, ).…”

Section: Discussionmentioning

confidence: 99%

Carnosine treatment during human semen processing by discontinuous density gradient

et al. 2019

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The aim of this article was to evaluate the effects of different concentrations of carnosine added during human semen processing. Semen samples from 34 patients were submitted to processing by discontinuous density gradient centrifugation without (control) or with different concentrations of carnosine supplementation as follows: (a) 20 mM of carnosine supplementation on the layers of Percoll; and (b) 50 mM carnosine supplementation. Sperm samples were then washed with human tubal fluid medium and evaluated according to sperm kinetics and functional assessment. For statistical analysis, data were evaluated by a general linear model or a Friedman test, whenever appropriate. The 50 mM carnosine supplementation led to improved sperm mitochondrial activity when compared to untreated samples. Motility variables, such as percentage of motile and progressively motile spermatozoa, average path velocity, straight line velocity, curvilinear velocity and linearity, showed an improvement after semen processing irrespective of carnosine supplementation. Both concentrations of carnosine increased the beat‐cross frequency (BCF) when compared to samples before processing. We conclude that carnosine supplementation in semen samples benefits sperm mitochondrial activity and BCF.

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The relationship between sperm DNA fragmentation, free radicals and antioxidant capacity with idiopathic repeated pregnancy loss

Cited by 51 publications

References 25 publications

Malaysian propolis and metformin mitigate subfertility in streptozotocin‐induced diabetic male rats by targeting steroidogenesis, testicular lactate transport, spermatogenesis and mating behaviour

Malaysian propolis and metformin mitigate subfertility in streptozotocin‐induced diabetic male rats by targeting steroidogenesis, testicular lactate transport, spermatogenesis and mating behaviour

Effects of the normal sperm morphology rate on the clinical and neonatal outcomes of conventional IVF cycles

Carnosine treatment during human semen processing by discontinuous density gradient

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