The Relationship between Small-Intestinal Bacterial Overgrowth and Intestinal Permeability in Patients with Irritable Bowel Syndrome

Park, Jung Ho; Park, Dong Il; Kim, Hong Joo; Cho, Yong Kyun; Sohn, Chong Il; Jeon, Woo Kyu; Kim, Byung Ik; Won, Kyoung Hee; Park, Soon Min

doi:10.5009/gnl.2009.3.3.174

Cited by 37 publications

(34 citation statements)

References 34 publications

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“…Studies using the urine excretion of 50 Cr-EDTA taken with a 200 kcal nutrient test meal showed elevations in both 0-6 h and 6-24 h urinary collections, which approximate to small and large bowel permeability in both PI-IBS and IBS with constipation (IBS- Dysbiosis in microbial flora resulting in the lack of signals to maintain barrier function Bansal et al, 36 Fukuda et al, 37 Hamer et al 37 Dysbiosis resulting in an increase in signals that break the barrier . 54 Another study using the ratio of PEG 3500-400 urine excretions showed increases in both IBS-D and IBS-C. 55 Using intestinal biopsies placed directly in mini-Ussing chambers, Piche and colleagues 15 demonstrated that the paracellular permeability to FITC sulfonic acid was significantly increased regardless of IBS subtype in a small sample (12 IBS patients). They also showed a reduction in a tight junction protein ZO-1 mRNA, but no difference in occludin.…”

Section: Evidence For Increased Permeability In Ibs Patientsmentioning

confidence: 99%

“…Studies using the urine excretion of 50 Cr‐EDTA taken with a 200 kcal nutrient test meal showed elevations in both 0–6 h and 6–24 h urinary collections, which approximate to small and large bowel permeability in both PI‐IBS and IBS with constipation (IBS‐C) 54 . Another study using the ratio of PEG 3500–400 urine excretions showed increases in both IBS‐D and IBS‐C 55 . Using intestinal biopsies placed directly in mini‐Ussing chambers, Piche and colleagues 15 demonstrated that the paracellular permeability to FITC sulfonic acid was significantly increased regardless of IBS subtype in a small sample (12 IBS patients).…”

Section: Introductionmentioning

confidence: 98%

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Intestinal barrier function in health and gastrointestinal disease

Camilleri¹,

Madsen²,

Spiller³

et al. 2012

Neurogastroenterology Motil

669

496

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Defects in intestinal barrier function are associated with diseases of the gastrointestinal (GI) tract. There is growing evidence that increases in intestinal permeability plays a pathogenic role in diseases, such as inflammatory bowel disease (IBD) and celiac disease, and functional bowel disorders, such as irritable bowel syndrome (IBS). This review takes a unique translational approach to discuss the physiological and pathophysiological mechanisms involved in the regulation of intestinal barrier function in IBS. The review summarizes the components of the intestinal barrier including the tight junction complex within the epithelium, and the methods used to assess gut permeability both in vitro and in vivo. Throughout the review, the authors have attempted to critically review the latest research from both experimental animal models and human studies to appraise whether intestinal barrier dysfunction is a primary cause of functional GI disorders, such as IBS....

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Section: Evidence For Increased Permeability In Ibs Patientsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Intestinal barrier function in health and gastrointestinal disease

Camilleri¹,

Madsen²,

Spiller³

et al. 2012

Neurogastroenterology Motil

669

496

View full text Add to dashboard Cite

show abstract

“…Another study showed unchanged claudin-1 protein expression in IBS-C as compared to healthy individuals [7]. In vivo measurements of intestinal permeability using urinary excretion of orally ingested, poorly-absorbable saccharides have also shown variable results in IBS-C. One study has shown increased permeability using polyethylene glycol (PEG) 3350/400 [8], another using PEG 400/1500/4000 showed no difference [9] and one using 51 Cr-EDTA showed decreased permeability [10]. Variability in timing of urine collection and dietary contamination often limits interpretation from these in vivo assays.…”

Section: Introductionmentioning

confidence: 99%

Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function

Peters

Edogawa

Sundt

et al. 2017

American Journal of Gastroenterology

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Objective To determine if constipation predominant IBS (IBS-C) is associated with changes in intestinal barrier and secretory function. Design 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g 13C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and E. coli Bio-Particle translocation), mucosal secretion (basal and ACh evoked short circuit current, Isc), in vivo duodenal mucosal impedance, circulating endotoxins and colonic tight junction gene expression. Results There were no differences in the in vivo measurements of barrier function between IBS-C and healthy: cumulative excretion of 13C mannitol (0–2 hr mean (SEM); IBS-C: 12.1 (0.9) mg vs healthy: 13.2 (0.8) mg) and lactulose (8–24 hr; IBS-C: 0.9 (0.5) mg vs healthy: 0.5 (0.2) mg); duodenal impedance IBS-C: 729 (65) Ω vs healthy: 706 (43) Ω; plasma mean endotoxin activity level IBS-C: 0.36 (0.03) vs healthy: 0.35 (0.02); and in colonic mRNA expression of occludin, ZO 1–3, and claudins 1–12, 14–19. Ex vivo findings were consistent, with no group differences: duodenal TMR (IBS-C: 28.2 (1.9) Ω*cm2 vs healthy: 29.8 (1.9) Ω*cm2) and colonic TMR (IBS-C: 19.1 (1.1) Ω*cm2 vs healthy: 17.6 (1.7) Ω*cm2); FITC Dextran (4 kDa) and E. coli Bio- Particle flux. Colonic basal Isc was similar, however, duodenal basal Isc was lower in IBS-C (43.5 (4.5) µA*cm2) vs healthy (56.9 (4.9) µA*cm2), p=0.05. ACh evoked ΔIsc was similar. Conclusions Females with IBS-C have normal colonic barrier and secretory function. Basal duodenal secretion is decreased in IBS-C.

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“…Increased gut permeability and defects in intestinal barrier function are associated with several functional gastrointestinal disorders such as irritable bowel syndrome (IBS) (1). Studies using urine excretion showed increased intestinal permeability to 50 Cr-EDTA and low molecule polyethylene glycol (PEG-400) in patients with IBS (2, 3). More recently, Rao and his colleagues (4) reported that both small bowel and colon permeability were increased in IBS patients with diarrhea by measuring saccharides lactulose and mannitol secretion in urine collections.…”

Section: Introductionmentioning

confidence: 99%

“…1 Studies using urine excretion showed increased intestinal permeability to 50 Cr-EDTA and low molecule polyethylene glycol (PEG-400) in patients with IBS. 2,3 More recently, Rao et al 4 reported that both small bowel and colon permeability were increased in IBS patients with diarrhea by measuring saccharides lactulose and mannitol secretion in urine collections. Another study using intestinal biopsies in Ussing chambers demonstrated that colonic paracellular permeability to sulfonic acid was significantly increased in IBS patients regardless of IBS subtype.…”

Section: Introductionmentioning

confidence: 99%

Corticosterone mediates stress‐related increased intestinal permeability in a region‐specific manner

Zheng

et al. 2013

Neurogastroenterology Motil

118

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Background Chronic psychological stress (CPS) is associated with increased intestinal epithelial permeability and visceral hyperalgesia. It is unknown whether corticosterone (CORT) plays a role in mediating alterations of epithelial permeability in response to CPS. Methods Male rats were subjected to 1-hour water avoidance (WA) stress or subcutaneous CORT injection daily for 10 consecutive days in the presence or absence of corticoid-receptor antagonist RU-486. The visceromotor response (VMR) to colorectal distension (CRD) was measured. The in situ single-pass intestinal perfusion was used to measure intestinal permeability in jejunum and colon simultaneously. Key Results We observed significant decreases in the levels of glucocorticoid receptor (GR) and tight junction proteins in the colon but not the jejunum in stressed rats. These changes were largely reproduced by serial CORT injections in control rats and were significantly reversed by RU-486. Stressed and CORT-injected rats demonstrated a 3-fold increase in permeability for PEG-400 (MW) in colon but not jejunum and significant increase in VMR to CRD, which was significantly reversed by RU-486. In addition, no differences in permeability to PEG-4,000 and PEG-35,000 were detected between control and WA groups. Conclusions & Inferences Our findings indicate that CPS was associated with region-specific decrease in epithelial tight junction protein levels in the colon, increased colon epithelial permeability to low-molecular weight macromolecules which were largely reproduced by CORT treatment in control rats and prevented by RU-486. These observations implicate a novel, region-specific role for CORT as a mediator of CPS-induced increased permeability to macromolecules across the colon epithelium.

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The Relationship between Small-Intestinal Bacterial Overgrowth and Intestinal Permeability in Patients with Irritable Bowel Syndrome

Cited by 37 publications

References 34 publications

Intestinal barrier function in health and gastrointestinal disease

Intestinal barrier function in health and gastrointestinal disease

Constipation-Predominant Irritable Bowel Syndrome Females Have Normal Colonic Barrier and Secretory Function

Corticosterone mediates stress‐related increased intestinal permeability in a region‐specific manner

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