The relationship between platelet endothelial cell adhesion molecule-1 and paraquat-induced lung injury in rabbits

Abstract

BACKGROUND: Platelet endothelial cell adhesion molecule-1 (PECAM-1), also known as CD31, is mainly distributed in vascular endothelial cells. Studies have shown that PECAM-1 is a very signifi cant indicator of angiogenesis, and has been used as an indicator for vascular endothelial cells. The present study aimed to explore the relationship between the expression of PECAM-1 and the degree of acute lung injury (ALI) and fi brosis in paraquat (PQ) induced lung injury in rabbits. METHODS: Thirty-six adult New Zealand rabbits were randomly divided into three groups (12 rabbits in each group) according to PQ dosage: 8 mg/kg (group A), 16 mg/kg (group B), and 32 mg/kg (group C). After PQ infusion, the rabbits were monitored for 7 days and then euthanized. The lungs were removed for histological evaluation. Masson staining was used to determine the degree of lung fibrosis (LF), and semi-quantitative immune-histochemistry analysis to determine the expression of PECAM-1. Pearson's product-moment correlation analysis was performed to evaluate the relationship between the expression of PECAM-1 and the extent of lung injuries expressed by ALI score and degree of LF. RESULTS: Rabbits in the three groups showed apparent poisoning. The rabbits survived longer in group A than in groups B and C (6.47±0.99 days vs. 6.09±1.04 days vs. 4.77±2.04 days) (P<0.05). ALI score was lower in group A than in groups B and C (8.33±1.03 vs. 9.83±1.17 vs. 11.50±1.38) (P<0.05), and there was statistically significant difference between group B and group C (P=0.03). LF was slighter in group A than in groups B and C (31.09%±2.05 % vs. 34.37%±1.62 % vs. 36.54%±0.44%) (P<0.05), and there was statistically significant difference between group B and group C (P=0.026). The PEACAM-1 expression was higher in group A than in groups B and C (20.31%±0.70% vs. 19.34%±0.68% vs. 18.37%±0.46%) (P<0.05), and there was statistically signifi cant difference between group B and group C (P=0.017). Pearson's correlation analysis showed that the expression of PECAM-1 was negatively correlated to both ALI score (Coe=-0.732, P=0.001) and degree of LF (Coe=-0.779, P<0.001).

The Correlation between Traditional Chinese Medicine Constitution and Hyperuricemia and Gout: A Systematic Review and Meta-Analysis

“…After duplicates were removed, 392 articles were retrieved for further assessment. And by title and abstract screening, followed by the above selection criteria, 39 studies were finally included [ 9 – 46 ] ( Figure 1 ), with 19 cross-sectional studies [ 10 – 17 , 19 – 21 , 23 , 24 , 26 , 27 , 29 , 30 , 32 , 33 ] on HUA, 9 cross-sectional studies about gout [ 18 , 34 – 37 , 39 , 41 , 43 , 44 ], 6 case-control studies about HUA [ 9 , 22 , 25 , 28 , 31 , 45 ], and 4 case-control studies about gout [ 38 , 40 , 42 , 46 ], and 1 cross-control study [ 18 ] on both HUA and gout. The fundamental information of the studies is shown in Table 1 .…”

“…Three studies [ 23 , 24 ] reported measures taken to control confounding factors. One study [ 37 ] had follow-up but did not report the results. But 3 [ 16 , 27 , 30 ] studies did not report the time that studies started and ended.…”

“…In south China, the results of 15 studies [ 10 – 13 , 15 , 17 , 19 , 20 , 22 , 24 , 25 , 27 , 28 , 30 – 32 ] on HUA and 5 studies [ 37 , 38 , 41 , 42 , 44 ] on gout showed that 3 types of TCM constitution with the highest proportions of patients with HUA were PDC 25% (95% CI = (0.209, 0.291)), DHC 21% (95% CI = (0.130, 0.291)), and QDC 16% (95% CI = (0.118, 0.194)), while the 3 types of TCM constitution with the highest proportions in patients with gout were DHC 34% (95% CI = (0.292, 0.388)), PDC 27% (95% CI = (0.213, 0.328)), and BSC 18% (95% CI = (0.143, 0.223)).…”

ITRAQ-based quantitative proteomics analysis of forest musk deer with pneumonia

“…MMP9 acted as a potentially protective factor against Streptococcus pneumonia infection ( 82 , 83 ). PECAM1, an endothelial cell adhesion molecule, played a potentially protective role in lung injury and acute respiratory distress syndrome ( 84 , 85 ). THBS1 also has been found to be implicated in the pathogenesis of gram-positive bacteria and the development of lung injury ( 86 , 87 ).…”

Effect of SP-A/B in lipoic acid on acute paraquat poisoning

“…[21] The increase of TNF-α could restrain the generation of active substance of the alveolar surface, reduce the synthesis of phosphatidy choline (PtdCho), and promote the degradation of active substance PtdCho of the alveolar surface. [20] LA could reduce the inhibition of TNF-α via the water channel protein-5 and the degree of pulmonary edema in addition to the improvement of pulmonary compliance. Surface active substance PtdCho was degraded increasingly, whereas alveolar surface active substance decreased.…”