The Relationship between Growth Rate, Differentiation and Alkaloid Accumulation in Cell Cultures

Lindsey, Keith; Yeoman, M. M.

doi:10.1093/jxb/34.8.1055

Cited by 172 publications

(57 citation statements)

References 14 publications

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“…There is a competition between primary and secondary metabolism for common precursors, according to whether culture conditions permit rapid or slow growth. The evidence of Phillips and Henshaw (1977) and Lindsey and Yeoman (1983) directly suggests a competition between two pathways. It is proposed that alternative pathways such as those of protein synthesis and phenolic or alkaloid synthesis will be active depending on whether one of the reactions is kinetically favourable.…”

Section: Tissue Culturesmentioning

confidence: 97%

Strawberry Flavour: Analysis and Biosynthesis

Zabetakis

Holden

1997

J. Sci. Food Agric.

201

137

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Section: Tissue Culturesmentioning

confidence: 97%

Strawberry Flavour: Analysis and Biosynthesis

Zabetakis

Holden

1997

J. Sci. Food Agric.

201

137

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Section: Introductionmentioning

confidence: 96%

“…Studies with mature plants also reveal this type of developmental control (Westekemper et al, 1980;Frischknecht et al, 1986). Furthermore, alkaloid biosynthesis in cell suspension cultures appears to be coordinated with cytodifferentiation (Kutchan et al, 1983;Lindsey and Yeoman, 1983).Vindoline biosynthesis in Catharanthus roseus also appears to be under this type of developmental control (Westekemper et al, 1980;Constabel et al, 1982). In the leaves of C. roseus , vindoline is enzymatically coupled with catharanthine to produce the powerful cytotoxic dimeric alkaloids vinblastine and vincristine (Svoboda and Blake, 1975).…”

mentioning

confidence: 98%

Multicellular Compartmentation of Catharanthus roseus Alkaloid Biosynthesis Predicts Intercellular Translocation of a Pathway Intermediate

1999

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In situ RNA hybridization and immunocytochemistry were used to establish the cellular distribution of monoterpenoid indole alkaloid biosynthesis in Madagascar periwinkle ( Catharanthus roseus ). Tryptophan decarboxylase (TDC) and strictosidine synthase (STR1), which are involved in the biosynthesis of the central intermediate strictosidine, and desacetoxyvindoline 4-hydroxylase (D4H) and deacetylvindoline 4-O -acetyltransferase (DAT), which are involved in the terminal steps of vindoline biosynthesis, were localized. tdc and str1 mRNAs were present in the epidermis of stems, leaves, and flower buds, whereas they appeared in most protoderm and cortical cells around the apical meristem of root tips. In marked contrast, d4h and dat mRNAs were associated with the laticifer and idioblast cells of leaves, stems, and flower buds. Immunocytochemical localization for TDC, D4H, and DAT proteins confirmed the differential localization of early and late stages of vindoline biosynthesis. Therefore, we concluded that the elaboration of the major leaf alkaloids involves the participation of at least two cell types and requires the intercellular translocation of a pathway intermediate. A basipetal gradient of expression in maturing leaves also was shown for all four genes by in situ RNA hybridization studies and by complementary studies with dissected leaves, suggesting that expression of the vindoline pathway occurs transiently during early leaf development. These results partially explain why attempts to produce vindoline by cell culture technology have failed. INTRODUCTIONThe organs forming the plant body consist of several different cell types that are organized in relation to each other and that confer specific functions to the resulting organ. Each cell type emerges from an undifferentiated meristem according to a sophisticated and partially understood developmental program (Sylvester et al., 1996;von Arnim and Deng, 1996). The commitment to differentiate into specialized structures involves the perception by cells in the meristem of a complex array of signals, which communicate cellular age, position in relation to other cells, and hormonal balance. Environmental factors, such as light and temperature, also play a critical role in modulating these signals throughout the process of organogenesis (Bernier, 1988;Dale, 1988;Sylvester et al., 1996).In addition to morphogenesis, developmental processes result in biochemical specialization of cells for the biosynthesis and/or accumulation of secondary metabolites, such as phenylpropanoids (Ibrahim et al., 1987;Reinold and Hahlbrock, 1997), monoterpenoids (Fahn, 1988;McCaskill et al., 1992), and alkaloids (Robinson, 1974(Robinson, , 1981Nessler and Mahlberg, 1977;Eilert et al., 1985;Hashimoto and Yamada, 1994;Facchini and De Luca, 1995). Studies with germinating seedlings have suggested that alkaloid biosynthesis and accumulation are associated with seedling development (Weeks and Bush, 1974;De Luca et al., 1986;Aerts et al., 1994). Studies with mature plants also reveal this ty...

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“…Medium optimization studies have singled out hormones, phosphate, nitrogen, and carbohydrates as components which potentially trigger the switch from primary to secondary metabolism (Moreno et al, 1995;Verpoorte et al, 1993). An opposing relationship between primary and secondary metabolism has been postulated by the observation that secondary metabolite production is low or absent when growth is rapid (e.g., Lindsey and Yeomann, 1983;Payne et al, 1988;Yamakawa et al, 1983). Thus, medium components which promote high-growth rates often suppress secondary metabolite production Yamakawa et al, 1983).…”

Section: Introductionmentioning

confidence: 99%