The relationship between fluoride accumulation in tea plant and changes in leaf cell wall structure and composition under different fluoride conditions

Luo, Jinlei; Zhi, Yu; Li, Chunlei; Du, Yanxiu; Chen, Yuqiong

doi:10.1016/j.envpol.2020.116283

Cited by 16 publications

(3 citation statements)

References 53 publications

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“…The cell wall of alfalfa was extracted, as described by Luo et al [21]. Briefly, after treating for 6, 12, and 24 hours, the root segments at 0-5 mm and 5-10 mm of two materials were cut with sterilized blades and stored in a refrigerator at -80ºC to extract the cell wall.…”

Section: Methodsmentioning

confidence: 99%

“…Weigh 0.5g of sample into the digestion tank, add 10ml nitric acid, put it on the microwave digestion plate for digestion. After cooling, transfer it into a triangular flask, put it on the electric heating plate to drive out the acid, take it down and cool it when the solution is nearly Cell wall pectin was extracted, as described by Luo et al [21]. Pectin in cell wall powder was extracted by 4 mL of 0.5% ammonium oxalate buffer (containing 0.1% NaHB 4 , pH = 4.0).…”

Section: Methodsmentioning

confidence: 99%

“…Spectrophotometric method was employed to determine DM [5,21]. 50 μL of NaOH (1.5 mol L -1 ) was added to the same pectin extract (100 μL) (described in 2.7 section) for saponification reaction, which lasted for 30 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Aluminum Exposure Effect on Cell Wall Pectin Methyl Esterification in Alfalfa with Different Aluminum Tolerance

Jiang¹,

Ren²,

Zu³

et al. 2022

Pol. J. Environ. Stud.

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Aluminum (Al) toxicity is one of the factors limiting alfalfa production on acid soils. This study aims to investigate the different mechanisms in growth, Al content, pectin content, pectin methylesterase (PME) and the degree of pectin methyl-esterification (DM) in the two root segments of different ecotypes alfalfa during the time of Al stress. It was found interesting that lower root elongation and higher Al content were observed in the Al-sensitive ecotype compared to the Al-resistant ecotype of alfalfa. The Al accumulation increased with the increased of stress time. Meanwhile, the pectin and PME activity of cell wall significantly increased. While DM in Al-sensitive ecotype decreased, more free pectin acid residues on the cell wall of Al-sensitive ecotype. The Al content was higher in 0-5 mm root segments and the cell wall for both materials. The increase in pectin content and PME activity along with the decrease in DM in this segment could determine the alfalfa sensitivity to Al more effectively. The correlation analysis showed that there was a relationship between Al content, pectin content, PME activity of cell wall and DM. The different temporal dynamics and spatial differences caused the increase of Al content, pectin content and cell wall PME activity, changes of DM under Al toxicity cooperates to determine the difference of Al resistance that confers different ecotypes of alfalfa.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%