The Relationship Between Fatty Acid Composition and the Size of Adipocytes from Subcutaneous Adipose Tissue of Holstein Steers During the Fattening Period

He, Long; Sang-Gun, Roh; Oka, Hideyuki; Hidaka, Shigekazu; Matsunaga, Nobuyoshi; Hemmi, Hisashi

doi:10.2508/chikusan.68.838

Cited by 7 publications

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“…The percentage of MUFA of marbling flecks categorized as L was significantly greater than M and S (P < 0.05), suggesting that larger marbling flecks tend to have a greater MUFA. Lending support to our findings, He et al (1997) reported that large adipocytes had greater MUFA, and Yang et al (2006) reported that the diameters of adipocytes in large marbling flecks were larger than those of small flecks on average.…”

Section: Resultssupporting

confidence: 88%

“…The fatty acid profile was measured according to the method of He et al (1997) as follows: the target marbling (approximately 10 mg) was scraped off using a metal spatula and methylated in 5 mL of methanol containing 5% hydrochloric acid for 3 h at 100°C. Adipose tissues of whole rib eye and subcutaneous fat were sampled by wiping the surface of the rib eye or fat with polyester plates 2.5 cm in width.…”

Section: Fatty Acid Analysismentioning

confidence: 99%

“…The percentage of MUFA to total fatty acids is affected by factors such as sex, breed, sire, and deposit site (Zembayashi et al, 1995;Oka et al, 2002). He et al (1997) reported that the fatty acid composition of adipose tissue differed among the sizes of fat cells, even for an individual cow and within fat tissue from 1 animal. These results suggest that fatty acid composition differs by size and location of the marbling fleck in the rib eye, or by the cross-section through LM even for 1 cow.…”

Section: Introductionmentioning

confidence: 99%

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Relationships between monounsaturated fatty acids of marbling flecks and image analysis traits in longissimus muscle for Japanese Black steers1

Nakahashi

Maruyama

Seki

et al. 2008

Journal of Animal Science

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The percentage of MUFA to total fatty acids of beef differs among intermuscular, intramuscular, and subcutaneous fat even within an individual cow. Our objective was to investigate the variation of the percentage of MUFA by geometric and sectional change of marbling flecks in rib eye. Longissimus muscles of 8 Japanese Black steers from a common sire and a common maternal grand sire were used. Three slices (1 from rib roast and 2 from sirloin) from each animal were selected for analysis. Five marbling flecks from each slice were randomly taken to obtain the percentage of MUFA using gas chromatography. High-quality digital images of all slices were taken with a mirror-type camera. The area and location of each marbling fleck were calculated by image analysis. The marbling flecks were categorized by area [small <0.4 cm(2), medium 0.4 to 2.0 cm(2), large >2.0 cm(2)], by location (dorsal and ventral), and by slice section through the LM (front, middle, and back). The effects of classification according to the area, location, and slice section were statistically significant (P < 0.05) for the percentage of MUFA. Least squares means of the percentage of MUFA for marbling flecks of sizes small, medium, and large were 56.8, 58.4, and 60.2%, respectively, indicating that larger marbling flecks had greater MUFA (P < 0.05). Those of dorsal, ventral, front, middle, and back were 59.1, 57.8, 55.4, 59.9, and 60.1%, respectively. The percentages of MUFA of the marbling flecks located in the dorsal part were greater than those in the ventral part (P < 0.05). The percentages of MUFA from middle and back were greater than those from front (P < 0.01). We suggest that the area, location, and slice section of marbling would be the determining factors for the percentage of MUFA of marbling.

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Section: Resultssupporting

confidence: 88%

Section: Fatty Acid Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Relationships between monounsaturated fatty acids of marbling flecks and image analysis traits in longissimus muscle for Japanese Black steers1

Nakahashi

Maruyama

Seki

et al. 2008

Journal of Animal Science

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“…The fatty acid profile was determined according to the method of He et al (1997). In brief, approximately 10 mg of each adipose tissue sample were methylated in 3 mL of methanol containing 5% hydrochloric acid for 3 h at 100°C.…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Relationship between adipose maturity and fatty acid composition in various adipose tissues of Japanese Black, Holstein and Crossbred (F1) steers

Lee¹,

Yamamoto

Jeong³

et al. 2011

Animal Science Journal

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The amount of monounsaturated fatty acid (MUFA) is intimately related to adipose softness, melting point (MP) and flavor in beef. Stearoyl-CoA desaturase (SCD) is a main gene involved in MUFA synthesis. Mature adipose tends to be highly saturated, whereas immature or maturing adipose is highly unsaturated when chronologically based, so the degree of non-saturation can be an index of adipose maturity. In this study, three different adipose tissues (coelomic (CL), perirenal (PR), and subcutaneous (SC)) from three beef breeds with differing slaughter ages (Japanese Black (29.5 months), Holstein (20.1 month), and F1 crossbreed (25.6 months)) were examined to: (i) determine adipose maturity level as indexed by MUFA %; and (ii) determine SCD and other lipogenic gene messenger RNA (mRNA) expression levels in relation to unsaturated fatty acid content. Fatty acid composition was significantly different between adipose tissues (P < 0.05). MUFA amount was high in the following order: SC > CL > PR. This pattern corresponded to SCD mRNA expression profile showing higher expression in SC than CL and PR. However, Japanese black cattle are an exception with CL adipose containing similar UFA % as SC adipose, yet having the lowest SCD mRNA expression level among all adipose tissues tested. Therefore, SCD mRNA expression and MUFA % appear to be directly related; however, differences in SCD mRNA expression among three adipose tissues may reflect differences in the fat development characteristics affected by chronological age of the cattle breeds.

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“…Cellular lipid accumulation in mature adipocytes depends on both lipogenesis and lipolysis and may alter cell size and the cellular fatty acid composition. It was found [33] that in growing and fattening beef cattle the composition of cellular monounsaturated fatty acids such as C14:1, C16:1 and C18:1 had strong positive correlation with mean size of adipocytes, while that of saturated fatty acids C18:0 and C16:0 had a negative correlation with it. A similar correlation was found between the cellular fatty acids composition and adipocytes distributed in the same fat pad that excluded variations caused by animal, feed and environment [34].…”

Section: Discussionmentioning

confidence: 99%

Effect of a Seaweed Extract on Fatty Acid Accumulation and Glycerol‐3‐Phosphate Dehydrogenase Activity in 3T3‐L1 Adipocytes

Wang

You

et al. 2008

Lipids

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This study was to determine the effect of a seaweed Ascophyllum nodosum extract (SE) containing 220 mg g(-1) phlorotannins on differentiation and fatty acid accumulation in differentiating 3T3-L1 adipocytes. 3T3-L1 cells (2 x 10(4) mL(-1)) were seeded to 24-well plates and proliferated to reach confluence and then were treated with media containing 0, 12.5, 25, 50, 75 and 100 mug mL(-1) SE for 8 days. Dexamethasone, methyl-isobutylxanthine and insulin (DMI) were added to the media in the first 2 days to induce cell differentiation. On day 8 the adipocytes were harvested for measuring cellular fatty acid concentration and the activity of glycerol-3-phosphate dehydrogenase (GPDH). It was found that treatment with SE increased (P < 0.01, n = 6) cellular myristoleic acid (C14:1), palmitoleic acid (C16:1) and oleic acid (C18:1) and total monounsaturated fatty acids (MUFA) without significantly affecting the cell number and saturated fatty acid (SFA). Ratios of MUFA/SFA, C14:1/C14:0, C16:1/C16:0 and C18:1/C18:0 in cellular lipids increased (P < 0.05, n = 6) with the SE treatment in a dose dependent manner (P < 0.001). Treatment with 75 microg mL(-1) SE depressed (P < 0.05) cellular GPDH activity. The results indicate that the biological factors in the SE may be involved in differentiation and MUFA accumulation in adipocytes.

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The Relationship Between Fatty Acid Composition and the Size of Adipocytes from Subcutaneous Adipose Tissue of Holstein Steers During the Fattening Period

Abstract: The fatty acid composition and the diameter of subcutaneous adipocytes from 13Holstein steers at different fattening stages were investigated.

Cited by 7 publications

References 0 publications

Relationships between monounsaturated fatty acids of marbling flecks and image analysis traits in longissimus muscle for Japanese Black steers1

Relationships between monounsaturated fatty acids of marbling flecks and image analysis traits in longissimus muscle for Japanese Black steers1

Relationship between adipose maturity and fatty acid composition in various adipose tissues of Japanese Black, Holstein and Crossbred (F1) steers

Effect of a Seaweed Extract on Fatty Acid Accumulation and Glycerol‐3‐Phosphate Dehydrogenase Activity in 3T3‐L1 Adipocytes

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