The relationship between enzyme activity and neuroglia in plaques of multiple sclerosis

Histochemistry of enzyme response to trauma in the neocortex and corpus callosum of developing rat brain

“…In the corpus callosum the 20 day old rat showed the first distinct oxidative enzyme reaction along nerve fibres where lines of reactive oligodendrocytes receded from the site of injury similar to a reaction reported at the periphery of plaques of multiple sclerosis (Ibrahim and Adams, 1963;Friede and Knoller, 1964). However, the responses of the oxidative enzymes were dissimilar; LDH activity was less intense in the corpus callosum than in neocortex, G6-PDH activity was equally intense in both regions, while a-GPDH activity was also equally intense but within many fewer glial cells in the corpus callosum compared with neocortex.…”

Histochemistry of the neuroglia and myelin in experimental cerebral oedema

“…The integrity of the myelin is clearly demonstrated by the normal staining of white matter with the OTAN method, including the absence of Marchi-positive breakdown products (cholesterol esters), and by the absence of activated lipid-laden microglia (gitter cells). Magee et al (1957) (Ibrahim and Adams, 1963: Ibrahim et al, 1963Adams, Ibrahim, and Leibowitz, 1965; see also Friede, 1961;Friede and Knoller, 1964;van Houten and Friede, 1961).…”

“…In previous communications in this Journal we have described a characteristic neuroglial reaction that accompanies or precedes demyelination in multiple sclerosis and experimental cyanide encephalopathy, namely, oligodendroglial hyperplasia, hypertrophy, and enzymic hyperactivity (Ibrahim and Adams, 1963;Ibrahim, Briscoe, Bayliss, and Adams, 1963). The purpose of this present paper is to determine whether similar oligodendroglial and myelin changes occur in experimentally-produced cerebral oedema.…”

“…Sections were stained by haematoxylin and eosin and by metal impregnation methods for oligodendrocytes, astrocytes, microglia, and neurones (for technical details see Ibrahim and Adams, 1963).…”

Histochemical Study of Anterograde Fiber Degeneration and Transneuronal Changes in the Optic System of the Rabbit

“…Firede (9), and Ibrahim and Adams (16) reported that in cases of multiple sclerosis an increase in neuroglial count (predominantly oligodendrocytes) and increased OEA were found at the edge of active plaques. Ibrahim et al (17) also reported that the demyelinating lesion in experimental cyanide encephalopathy was preceded by hypertrophy, apparent hyperplasia and increased enzyme activity of oligodendrocytes.…”