The Relationship between Development of Lung Inflammation and Changes in Bone Marrow Populations in Guinea-Pigs following Inhaled Antigen Challenge

Barton, H.J.; Bond, Allen; Boot, John R.; Brandon, D. R.; O’Brien, Aidan

doi:10.1159/000235527

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…The present study went further in demonstrating the CD4+ nature of the T cells infiltrating the bronchial mucosa, by using the monoclo nal antibody H155. Other recent studies, using the same antibodies, showed that T cells increased in the BAL fluid after antigen challenge in immunized guinea pigs, but found no increase in the CD4+ population [13]. In conclusion, the expansion of the CD4+ population and eosinophils showed here in correlation with the devel opment of BHR previously demonstrated in this model opens new possibilities for the investigation of the immunopathogenesis of asthma and associated conditions and of its pharmacological modulation.…”

Section: Discussionsupporting

confidence: 75%

Booster-Dependent Alterations of the Subsets of T Lymphocytes and Eosinophils in the Bronchi of Immunized Guinea Pigs

Silva

Pretolani

Bachelet

et al. 1992

Int Arch Allergy Immunol

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We investigated whether guinea pigs sensitized to ovalbumin show changes in T cells in the bronchial wall and whether they correlate with eosinophil migration. Animals received two injections of 10 µg ovalbumin in Al(OH)₃ with a 2-week interval. Lungs were resected and frozen and cryostat sections stained with monoclonal antibodies recognizing T cells and subsets. Eosinophil peroxidase staining was used to identify this cell type. Sections stained with each marker were read ‘blind’ and cells enumerated in the bronchial lamina propria. A large number of T cells, mainly of CD4+ subset, were recruited into the bronchi 7 days after the booster injection of the antigen, in contrast to nonimmunized or nonboosted animals. These changes were also accompanied by an infiltration of eosinophils in boosted animals and suggest that T cells and/or their products play an important role in the development of bronchial hyperreactivity in this model.

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Section: Discussionsupporting

confidence: 75%

Booster-Dependent Alterations of the Subsets of T Lymphocytes and Eosinophils in the Bronchi of Immunized Guinea Pigs

Silva

Pretolani

Bachelet

et al. 1992

Int Arch Allergy Immunol

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“…Glucocorticoids are widely used for the treatment of allergic disorders (Schleimer, 1990; Barnes, 1995), and indeed dexamethasone (Dex) is routinely used to characterize in vivo models of allergic inflammation. For instance, administration of this steroid to guinea‐pigs or rats profoundly affected the infiltration of eosinophils into the trachea following allergen challenge (Barton et al , 1991; Elwood et al , 1992). In addition, Dex prevented eosinophil accumulation in tissue sites other than within the respiratory tract such as the mouse peritoneal cavity or rat pleural cavity (Spicer et al , 1986; Zuany‐Amorim et al , 1993; Bozza et al , 1993).…”

Section: Introductionmentioning

confidence: 99%

A novel murine model of allergic inflammation to study the effect of dexamethasone on eosinophil recruitment

Das

Flower

Hellewell

et al. 1997

British J Pharmacology

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1 We have developed a novel model of allergen-induced eosinophil extravasation into mouse air-pouches following sensitization and challenge with ovalbumin (Ova). This model was used to investigate the mechanism(s) underlying the anti-in¯ammatory action of the glucocorticoid hormone dexamethasone (Dex). 2 Injection of 10 mg Ova into 6-day-old dorsal air-pouches of mice sensitized to the same antigen provoked an intense cell accumulation as early as 6 h post-challenge (0.08+0.03 and 4.0+1.0610 5 leucocytes in saline and Ova-treated air-pouches, respectively), maximal at 24 h (0.02+0.01 and 6.0+0.8610 5 leucocytes in saline and Ova-treated air-pouches, respectively) and persisted up to 48 h. At the 24 h time-point, the cellular in®ltrate consisted of 37% eosinophils, 18% neutrophils and 45% mononuclear cells, as assessed by histological examination. The same ratio of eosinophil/neutrophil was obtained by¯uorescence-activated cell sorting (FACS) analysis, since 72% of the polymorphonuclear (PMN) population was positive for very-late antigen-4 (VLA-4) expression. 3 Subcutaneous (s.c.) administration of Dex (50 or 100 mg per mouse, 71 h) inhibited eosinophil accumulation into Ova challenged air-pouches by about 70% (P50.05) and 75% (P50.05), respectively, when compared to controls. Cell accumulation measured at 48 h after Ova injection was also signi®cantly reduced (775%) by Dex administration at the 24 h time-point (n=12, P50.05). 4 Eosinophil numbers in the bone marrow and blood were quantitated. We found that the sensitization protocol induced a 3 fold increase in eosinophil numbers in the bone marrow (naive mice: 2.7+0.3610 5 ; sensitized mice: 8.7+1.7610 5 , P50.05) and blood (naive mice: 0.5+0.2610 5 ; sensitized mice: 1.5+0.3610 5 , P50.01). However, 24 h following Ova challenge, the eosinophil numbers in the bone marrow had dropped (3.7+0.8610 5 ) with no change in the circulating pool, suggesting an equilibrium within the eosinophil pools had been reached. 5 Dex administration provoked a profound eosinopaenia in the blood of naive (5.2+1.5 to 0.9+0.6610 4 ) and sensitized mice (1.5+0.3 to 0.08+0.02610 5 ) at 4 h. This e ect was reversed within 24 h. Dex also inhibited the release of eosinophils from the bone marrow in response to Ova challenge. 6 We show for the ®rst time that eosinophils express the steroid-inducible protein lipocortin 1 (LC1). FACS analysis of eosinophils emigrated into the Ova challenged air-pouches revealed detectable LC1-like immunoreactivity (373610 3 ). These data were also substantiated by LC1 detection in circulating eosinophils of interleukin-5 transgenic mice (strain: CBA/Ca). However, s.c. injection of Dex (50 mg) did not alter LC1 levels in blood eosinophils, such that 235+21610 3 LC1-like molecules per cell were measured after vehicle treatment (n=5), and 224+8610 3 LC1-like molecules per cell were associated with this cell type 1 h after steroid treatment (n=5, not signi®cant). Finally, resident eosinophils (in the pleural cavity) were found to have much higher LC1 levels than ...

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“…A s has been found in guinea pigs by others [12,13], O A sensitisation followed by aerosol challenge led to pro nounced increases in the number and percent of eosino phils in their B A L fluids. However, there were significant differences between eosinophil numbers in parasite-naive guinea pigs with genetically determined differences in their responsiveness to the nematode parasite T. colubriformis.…”

Section: Discussionmentioning

confidence: 73%

Differences in Eosinophil Migration into the Lungs following Inhaled Antigen Challenge of Parasite-Naive Guinea Pigs with Genetically Determined Differences in Responsiveness to a Gastrointestinal Nematode Parasite

Gabor

Rothwell²

1994

Int Arch Allergy Immunol

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Parasite-naive guinea pigs with genetically determined differences in responsiveness to infection with the gastrointestinal nematode parasite Trichostrongylus colubriformis were sensitised to ovalbumin and later challenged by exposure to an ovalbumin aerosol. The resultant cellular migration into the lungs was assessed by histological examination of the lungs and enumeration of cells in bronchoalveolar lavage fluid 24 h, 72 h and 7 days later. Compared with parasite-low-responder guinea pigs, there were approximately 10 times more eosinophils in lavage fluid from parasite-high-responder animals but similar numbers of neutrophils.

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The Relationship between Development of Lung Inflammation and Changes in Bone Marrow Populations in Guinea-Pigs following Inhaled Antigen Challenge

Cited by 11 publications

References 12 publications

Booster-Dependent Alterations of the Subsets of T Lymphocytes and Eosinophils in the Bronchi of Immunized Guinea Pigs

Booster-Dependent Alterations of the Subsets of T Lymphocytes and Eosinophils in the Bronchi of Immunized Guinea Pigs

A novel murine model of allergic inflammation to study the effect of dexamethasone on eosinophil recruitment

Differences in Eosinophil Migration into the Lungs following Inhaled Antigen Challenge of Parasite-Naive Guinea Pigs with Genetically Determined Differences in Responsiveness to a Gastrointestinal Nematode Parasite

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