The relationship between denitrifying bacteria and methanogenic bacteria in a mixed culture system of acclimated sludges

Chen, Kuo-Cheng; Lin, Yu Shia

doi:10.1016/0043-1354(93)90113-v

Cited by 112 publications

(55 citation statements)

References 11 publications

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“…Our results strengthen this suggestion since acetate-and methanol-dependent methane production was also signi¢cantly inhibited by products of nitrate denitri¢cation. The same suggestion has also been made by Chen and Lin [7] who observed that 7.1 mM nitrate but 10 times less nitrite was required to completely inhibit methane production from methanol in methanogenic sludges. Regarding Chen and Lin's results [7], striking di¡eren-ces of the strength of inhibition by nitrate of methanol-dependent methane production were found between the methanogenic sludges and our cultures of M. mazei.…”

Section: In£uence Of Nitrogen Oxides On Methanogenic Activitysupporting

confidence: 52%

“…The same suggestion has also been made by Chen and Lin [7] who observed that 7.1 mM nitrate but 10 times less nitrite was required to completely inhibit methane production from methanol in methanogenic sludges. Regarding Chen and Lin's results [7], striking di¡eren-ces of the strength of inhibition by nitrate of methanol-dependent methane production were found between the methanogenic sludges and our cultures of M. mazei. M. mazei was only 46% inhibited by 14.3 mM nitrate in methanol medium (Table 1) while nitrate at only 0.71 mM exerted 63% inhibition on the methanogenic sludge studied by these authors.…”

Section: In£uence Of Nitrogen Oxides On Methanogenic Activitysupporting

confidence: 52%

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Effects of nitrogen oxides and denitrification by Pseudomonas stutzeri on acetotrophic methanogenesis by Methanosarcina mazei

Clarens

1998

FEMS Microbiology Ecology

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Nitrate and its reduced forms produced during denitrification, nitrite and nitrous oxide, were studied for their influence on methane production from acetate by Methanosarcina mazei. While 0.18 mM nitrite and 0.8% nitrous oxide in the gas phase completely suppressed methane production, 71.4 mM nitrate resulted in only 83.3% inhibition. Co-culture experiments showed that M. mazei growing with 15 mM nitrate produced methane from acetate until the denitrifying bacterium Pseudomonas stutzeri was inoculated and nitrate denitrification began. The presence of nitrous oxide in the gas phase after cessation of denitrification activity by P. stutzeri in co-cultures flasks prevented M. mazei resuming methane production. Nitrous oxide, instead of dinitrogen, was the end product of denitrification by P. stutzeri either in pure cultures or in co-cultures with M. mazei, probably because of the highly reduced culture conditions that were used. This study strongly suggests that acetatedependent methane production by M. mazei was inhibited by reduced nitrogen forms produced during bacterial nitrate denitrification, rather than by competition for acetate between denitrifying and methanogenic bacteria. These results are consistent with previous studies with H P /CO P methanogens. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

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Section: In£uence Of Nitrogen Oxides On Methanogenic Activitysupporting

confidence: 52%

Section: In£uence Of Nitrogen Oxides On Methanogenic Activitysupporting

confidence: 52%

Effects of nitrogen oxides and denitrification by Pseudomonas stutzeri on acetotrophic methanogenesis by Methanosarcina mazei

Clarens

1998

FEMS Microbiology Ecology

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“…Studies have generally focused on the inhibitory effects of nitrate in homogeneous systems. For example, in one study 17 in which a range of nitrate concentrations (1-200 mg N dm −3 ) was added to methanogenic sludge (no denitrifying activity) in shaken serum bottles, 81 and 100% inhibition of methane production was recorded at 10 and 100 mg N dm −3 , respectively. In mixed systems, that were inoculated with both methanogenic and denitrifying sludges, the relationship between denitrification and methanogenesis was controlled not only by the direct inhibitory action of nitrate addition, but also by the ratio of added carbon source (methanol) to nitrate.…”

Section: Introductionmentioning

confidence: 99%

The effects of nitrate and nitrate‐supplemented leachate addition on methanogenesis from Municipal Solid Waste

Watson-Craik

2004

J of Chemical Tech & Biotech

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The recirculation of nitrified leachate through landfill sites, followed by in situ denitrification, represents a novel and more sustainable approach for the removal of ammonia from leachate, prior to discharge. The effects of nitrate and leachate supplementation on methanogenesis in Municipal Solid Waste (MSW) were studied in batch cultures. The addition of a range of nitrate concentrations to MSW samples had an inhibitory effect on methanogenesis. The effects were dose-dependent, such that recovery of methane production was recorded within 5 and 23 days with added 100 and 750 mg NO 3 dm −3 , respectively. Even after 24 days, no recovery was observed in cultures challenged with 1000 mg NO 3 dm −3 . The enumeration of denitrifying bacteria in a range of fresh, actively methanogenic and aged, welldecomposed MSW confirmed the potential of MSW for rapid denitrification. Methanogenesis was not inhibited by the addition of leachate (20-100% strength) that contained high concentrations of VFAs. However, when the same leachate was supplemented with nitrate (250 mg NO 3 dm −3 ), methanogenesis was inhibited by the addition of leachate concentrations ≥20%, which was attributed to inhibition of denitrification by VFAs. Propionate accumulated, confirming the importance of methanogenesis as an electron sink. With the removal of nitrate and the recovery of methanogenesis, net propionate concentrations decreased.

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“…Competition for methanol between these two kinds of sludges could not be observed, whereas methanogenesis was suppressed as long as nitrate was made available in the mixed system. Adding nitrate also raised the redox potential of the system [33].…”

Section: Phase Ii: Influence Of Nitrate Addition On the Reactor Perfomentioning

confidence: 99%

“…An increase in redox potential hampers the methanogeneous activity of the microorganisms that transform VFAs to methane. The interaction between denitrification and methanogenesis, with methanol functioning as an electron donor, has been examined previously through the usage of a mixed culture system of denitrifying sludge and methanogenic sludge in an anaerobic reactor [33]. Competition for methanol between these two kinds of sludges could not be observed, whereas methanogenesis was suppressed as long as nitrate was made available in the mixed system.…”

Section: Phase Ii: Influence Of Nitrate Addition On the Reactor Perfomentioning

confidence: 99%

Effect of adding nitrate on the performance of a multistage biofilter used for anaerobic treatment of high-strength wastewater

Ghaniyari-Benis

Borja

Bagheri

et al. 2010

Chemical Engineering Journal

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This laboratory research was carried out to evaluate the performance of a multistage anaerobic biofilm reactor, with six compartments and a working volume of 70 L, for the treatment of a strong synthetic nitrogenous and high-strength wastewater at an operational temperature of 26ºC ± 0.5ºC. Initially, the performance of the reactor was studied when subjected to an increase in the hydraulic retention time (HRT) at a constant influent COD concentration of 10000 mg/L. Five different HRTs were studied: 0.25, 0.67, 1, 3 and 5 days, which were equivalent to 6, 16, 24, 72 and 120 hours, respectively . By increasing the HRTs from 6 h to 1 day, COD and BOD removal efficiencies were increased from 63% to 84% and from 66% to 87%, respectively. Moreover, at an HRT of 3 days, COD and BOD removal efficiencies were equal, reaching 93%. In the second phase of the research, the effect of adding nitrate with a concentration of 3000 mg/L at an influent organic loading rate of 10 g COD/L·day was researched on the reactor performance and the amount of biogas produced. Denitrification took place almost solely in the first three compartments of the reactor, with efficiencies of 85%, 95% and 98%, respectively. The nitrite produced was only detected in the first and second compartments at concentrations of 138 mg/L and 24 mg/L, respectively. In addition, no accumulation of nitrite was detected in the reactor. Furthermore, the denitrification caused an increase in the total volume of produced biogas from 102 L/d to 178 L/d.

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The relationship between denitrifying bacteria and methanogenic bacteria in a mixed culture system of acclimated sludges

Cited by 112 publications

References 11 publications

Effects of nitrogen oxides and denitrification by Pseudomonas stutzeri on acetotrophic methanogenesis by Methanosarcina mazei

Effects of nitrogen oxides and denitrification by Pseudomonas stutzeri on acetotrophic methanogenesis by Methanosarcina mazei

The effects of nitrate and nitrate‐supplemented leachate addition on methanogenesis from Municipal Solid Waste

Effect of adding nitrate on the performance of a multistage biofilter used for anaerobic treatment of high-strength wastewater

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