The relationship between chemiluminescence and lipid peroxidation in rat hepatic microsomes

Generation of Singlet Molecular Oxygen in Rat Liver Homogenate on Adding Autoxidized Linseed Oil

“…19 ) The chemiluminescence generated in the present model system was quenched with free radical scavengers (Table I), other than 10 2 quenchers, and the intensity had a linear relationship with the amount of AOLO added. These results would provide reasonable evidence for the mechanism in the model system being that the 10 2 was generated from the hydroperoxides in AOLO added to liver homogenate by the Russell reaction, as in the following scheme.…”

Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes

“…Chemiluminescence and lipid peroxidation were initiated by adding ascorbate to microsomes [18]. The microsomes (0.5 mg microsomes protein) with addition of CO total extract (0.1, 0.2, 0.3 and 0.4 mg) were incubated at 37 °C with 0.01 M phosphate buffer pH 7.4, 0.4 mM ascorbate, final vol.…”

Inhibitory Action of Oren-gedoku-to Extract on Enzymatic Lipid Peroxidation in Rat Liver Microsomes.

“…[8][9][10] It has been indicated that oxygen free radicals such as superoxide radical, hydroxyl radical, and singlet oxygen participate in enzymatic lipid peroxidation in rat liver microsomes. [27][28][29][30][31][32][33][34] We have observed that when the effects of superoxide dismutase, catalase, hydroxyl radical scavengers such as mannitol, sodium formate, and dimethylthiourea, and singlet oxygen quenchers such as histidine, diazabicyclooctane, and diphenylfuran, on enzymatic lipid peroxidation in rat liver microsomes used in the present study are examined, singlet oxygen quenchers inhibit peroxidation by more than 90%, while superoxide dismutase, catalase, and hydroxyl radical scavengers inhibit it by less than 20% (unpublished data). These results are consistent with those reported by Wright et al 34) who have proposed the involvement of singlet oxygen in the initiation of enzymatic lipid peroxidation in rat liver microsomes.…”