The relationship between cell membrane damage and lipid peroxidation under the condition of hypoxia‐reoxygenation: analysis of the mechanism using antioxidants and electron transport inhibitors

Abstract

We consecutively observed lipid peroxidation and cell membrane damage under the condition of hypoxia-reoxygenation (H/R) in cells and analyzed their mechanisms by using electron transport inhibitors and an antioxidant. In H/R experiments, lipid peroxidation and cell membrane damage were observed during the hypoxia phase. In the reoxygenation phase, lipid peroxidation stopped, while cell membrane damage did not. An antioxidant, n-acetylcystein (NAC), and potassium cyanide (KCN) inhibited lipid peroxidation and cell membrane damage, while rotenone did not inhibit either of them. Although antimycin A did not inhibit lipid peroxidation, it inhibited cell membrane damage during the hypoxia phase but not during the reoxygenation phase. These results suggested that lipid peroxidation can affect cell membrane damage as a trigger during the hypoxia phase and the generation of oxidative stress can vary depending on the inhibition locations in the electron transport system.

Oxidative and genetic responses induced by Δ-9-tetrahydrocannabinol (Δ-9-THC) to Dreissena polymorpha

“…Significant differences (two-way ANOVA, Fisher's LSD post-hoc test, *p b 0.05; **p b 0.01) were referred to the comparison between treated mussels and the corresponding control (time to time). oxidative stress situation, which might have led to the disruption of cell membrane integrity (Yajima et al, 2009) and loss of protein structural and functional efficiencies (Dalle-Donne et al, 2003, respectively. DNA is the other target biomolecule of cellular oxidative injury and many studies have highlighted that the increase of pollutant-induced ROS could undermine its integrity in different aquatic organisms (Regoli et al, 2002a,b;Mamaca et al, 2005), including zebra mussel (Parolini et al, 2010(Parolini et al, , 2011aParolini and Binelli, 2012).…”

Impact of phosphate on glyphosate uptake and toxicity in willow

“…As for catalase (CAT), APX and GPX are involved in scavenging hydrogen peroxide (H 2 O 2 ), a reactive oxygen species (ROS) whose accumulation leads to oxidative damage, i.e., lipid peroxidation [56]. Lipid peroxidation is an irreversible burst process leading to cell membrane destruction, with direct impact on cell membrane constitution and stability [57]. Therefore, by stimulating antioxidant systems, PO 4 3-alleviated glyphosateinduced oxidative stress, ensuring the stability of cell membranes, and then allowing glyphosate diffusion and uptake through roots.…”

A modified acid-fast staining method for rapid detection of Mycobacterium tuberculosis

“…Dioxogen is a strong oxidant that induces lipid peroxidation in the cytoderm. This peroxidation may damage the cytomembrane and increase membrane permeability (Yajima et al, 2009;Pandey et al, 2010). As a result, colorants can penetrate easily into the bacteria.…”