The relationship between acridine orange fluorescence of sperm nuclei and the fertilizing ability of human sperm

Hoshi, Kazuhiko; Katayose, Haruo; Yanagida, Kaoru; Kimura, Yasuyuki; Sato, Akira

doi:10.1016/s0015-0282(16)58581-1

Cited by 113 publications

(88 citation statements)

References 21 publications

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“…These embryos have been shown to arrest at the six-to eight-cell stage, coinciding with full activation of the embryonic genome. The impaired sperm maturation has also been associated with decreased pregnancy rates [35][36][37], failure of embryos to progress to the blastocyst stage in culture [38] and low pregnancy rates after ICSI treatment [29]. Furthermore, the spontaneous abortion rates of our study population, which were similar with those observed in previous studies [39], were increased in cases of semen samples with low percentages of mature spermatozoa.…”

Section: Discussionsupporting

confidence: 87%

Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

Lazaros

Vartholomatos

Hatzi

et al. 2011

J Assist Reprod Genet

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Purpose Sperm flow cytometry (SFC) was used to evaluate the association of sperm chromatin condensation and ploidy with fertilization, embryo development, pregnancy and abortion rates following IVF. Methods Conventional semen analysis was performed in one hundred fifty men, as well as SFC analysis, after acridine orange and propidium iodide staining, for the evaluation of sperm maturity and ploidy respectively. Conventional IVF was performed in all couples. Results Couples with low percentages of mature spermatozoa presented with lower fertilization rates (p < 0.005), lower rates of grade A embryos (p < 0.003) and lower pregnancy rates (p < 0.006), compared to couples with high percentages of mature spermatozoa. Couples with low total aneuploidy rates presented with higher fertilization rates (p < 0.007), higher rates of grade A embryos (p < 0.004) and higher pregnancy rates (p < 0.003), compared to couples with high total aneuploidy rates. Conclusions Sperm chromatin condensation and ploidy constitute critical parameters for the evaluation of semen samples before IVF and for the identification of cases in need of ICSI application.

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Section: Discussionsupporting

confidence: 87%

Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

Lazaros

Vartholomatos

Hatzi

et al. 2011

J Assist Reprod Genet

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“…Both SCSA and AOT measure the susceptibility of sperm nuclear DNA to acid-induced denaturation in situ by quantifying the metachromatic shift of acridine orange fluorescence from green (native DNA) to red (denatured DNA). Although the AOT has been used by some laboratories in an attempt to improve male fertility evaluations (Tejada et al, 1984;Foresta et al, 1989;Peluso et al, 1992;Hoshi et al, 1996;Duran et al, 1998), any predictive value by AOT for human fertility is still controversial. AOT relies on visual interpretation of fluorescing spermatozoa and debris that fall into a broad range of colors under microscopic examination (Duran et al, 1998).…”

Section: Sperm Chromatin Structure Assaymentioning

confidence: 99%

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Evenson

Larson

Jost

2002

Journal of Andrology

904

768

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The Sperm Chromatin Structure Assay (SCSA) was first described in the December 5, 1980 issue of Science (Evenson et al, 1980a). The data described in that article showed a significant difference between proven fertile and subfertility or infertility in men and bulls and the susceptibility to denaturation of their sperm nuclear DNA. A subsequent study (Evenson et al, 1984) described SCSA data obtained from men with testicular cancer. The data were heterogeneous, and begged the question, what was SCSA measuring? Great effort has been spent over the past 15 years on animal model systems, mostly related to dose-response toxicology experiments and large animal fertility trials. The results from these studies showed that SCSA was highly dose-responsive to toxicants, highly repeatable, and provided meaningful biological information on sperm nuclear DNA defects. The extensive data from nonhumans (Evenson et al, 1985(Evenson et al, , 1989a(Evenson et al, ,b, 1993a(Evenson et al, ,b,c, 1994Ballachey et al, 1987Ballachey et al, , 1988Evenson and Jost, 1993; Sailer et al, 1995a,b;Evenson, 1999a), and human toxicology and fertility studies (Evenson et al, 1978(Evenson et al, , 1980a(Evenson et al, ,b, 1984(Evenson et al, , 1991Evenson and Melamed, 1983;Evenson, 1999b;Larson et al, 1999Larson et al, , 2000Larson et al, , 2001 provide This work was supported in part by grant R827019 from the US Environmental Protection Agency, grants EPS-9720642 and OSR-9452894 from the National Science Foundation, and by SCSA Diagnostics, Inc. This is South Dakota Agricultural Experiment Station publication 3273 of the journal series.Correspondence to: Donald P. Evenson, Olson Biochemistry Laboratories, ASC 136, South Dakota State University, Brookings, SD 57006 (e-mail: donald evenson@sdstate.edu).Received for publication September 20, 2001; accepted for publication September 20, 2001. compelling evidence that SCSA will be useful in clinical human semen analysis (Figure 1).After reviewing current infertility center sites on the World Wide Web and approximately a dozen lay-oriented books on infertility and assisted reproductive technology (ART), it was amazing that not a single reference contained any information about the negative influence of fragmented sperm nuclear DNA on successful pregnancy outcome. Furthermore, few physicians are aware of scientific literature on this subject. Currently, if a spermatozoon has some motility as seen under a light microscope, and reasonable morphology, then many ART clinics assume that the sperm DNA must be fine. However, a number of cases have been documented in which couples have had multiple failed attempts at intracytoplasmic sperm injection (ICSI), even using donor eggs, and we found that the fragmentation of sperm DNA was above our threshold for less than 1% probability of achieving a successful pregnancy. Spermatozoa with defective DNA can fertilize an oocyte, produce high-quality early stage embryos, and then, in relationship to the extent of DNA damage, fail in producing a successful term ...

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“…The negative effects relating to rapid decreases in temperature, such as osmotic injury, cellular dehydration, intra-cellular ice crystal formation, and oxidative stress, also lead to damage to sperm DNA, chromatin instability and DNA denaturation [4][5][6][7][8]. Therefore, the reproductive outcome following fertilization with the sperm containing the damaged genetic material may be poor [8][9][10]. In cases where pregnancy is achieved using this sperm, the risk of abortion is increased because of the damaged genetic material in the sperm [11].…”

Section: Introductionmentioning

confidence: 99%

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

Petyim

Neungton

Thanaboonyawat

et al. 2014

J Assist Reprod Genet

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Purpose To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation. Methods Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group. Result (s) The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P<0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P<0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P<0.001). Conclusion (s) Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.

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The relationship between acridine orange fluorescence of sperm nuclei and the fertilizing ability of human sperm

Cited by 113 publications

References 21 publications

Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

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