The relations between absorbed dose, molecular mass distribution, and viscosity studied on aqueous solution of ovalbumin agglomerated by ? irradiation

Kuljanin, J.; Vujičić, G. M.; Radojčić, Marija B.; Milosavljevic, Bratoljub H.

doi:10.1002/1099-0488(20000701)38:13<1729::aid-polb60>3.0.co;2-m

Cited by 2 publications

(4 citation statements)

References 11 publications

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“…10 In our own previous research, we showed that cross-linking a 1% ovalbumin aqueous solution using radiation resulted in gel formation, whereby the dynamic viscosity increased from 2 mPa 3 s to an immeasurably high value. 11,12 This indicates that even though the viscosity reaches high values, cross-linking is able to proceed to completion in a relatively short time. Further, macromolecules have been used extensively as nanosized colloid and catalyst stabilizers, as indicated by Napper 13 and the works that frequently reference his review.…”

Section: ' Introductionmentioning

confidence: 97%

Macro- and Microscale Rheological Properties of Poly(vinyl alcohol) Aqueous Solutions

et al. 2011

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Electric conductivity measurements indicated that microviscosities of poly(vinyl alcohol), PVA, solutions [up to 10% (w/w)] are comparable to that of pure water (contrary to Walden's rule), but are different (25%) from those determined from diffusion-controlled reaction measurements that coincide with the values obtained using pure water. Energies of activation of fluidity of PVA solutions are found to increase with PVA concentration, whereas those of the diffusion-controlled reactions are independent of PVA concentration. By comparing the macro- and microviscosities, it was concluded that PVA aqueous solutions can be envisioned as dynamic systems comprising hydrated PVA molecules (that affect the macroviscosity) and "interconnected water pools" (located between macromolecules), the rheological properties of which are very similar to that of pure water. Fluorescence depolarization measurements indicated that pool sizes are relatively large, which was corroborated by DSC measurements that showed that each PVA hydroxyl group interacts with no more than two water molecules.

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Section: ' Introductionmentioning

confidence: 97%

Macro- and Microscale Rheological Properties of Poly(vinyl alcohol) Aqueous Solutions

et al. 2011

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“…The fragments were too large to be analyzed by SDS-PAGE, size-exclusion chromatography, or other methods for determining molar mass. In our previous research, we showed that there is a correlation between the molar mass of cross-linked protein and solution viscosity . Therefore, viscometry seemed to be the analytical method of choice, as it allowed for the determination of the fragment size distribution beyond 10 6 kDa .…”

Section: Resultsmentioning

confidence: 99%

“…In our previous research, we showed that there is a correlation between the molar mass of cross-linked protein and solution viscosity. 19 Therefore, viscometry seemed to be the analytical method of choice, as it allowed for the determination of the fragment size distribution beyond 10 6 kDa. 19 An Ostwald viscometer operative in the range 4000−20 000 mPa•s was initially used.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…19 Therefore, viscometry seemed to be the analytical method of choice, as it allowed for the determination of the fragment size distribution beyond 10 6 kDa. 19 An Ostwald viscometer operative in the range 4000−20 000 mPa•s was initially used. However, flow time measurements for the same sonicated sample were highly variable, which indicated that the flow was non-Newtonian; that is, some of the fragments were too large.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Sonolysis of High Macroviscosity Systems: Hen Albumen Hydrogel

et al. 2013

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To understand molecular effects of ultrasound on protein gels (cross-linked, hydrated macromolecular systems of immeasurably high macroviscosity, but low microviscosity), the thick fraction of hen albumen was sonicated. The immeasurably high viscosity of the intact thick fraction decreased to 2.5-4.0 mPa·s (depending on the sample) after a 12 min sonication (0.14 mM of radicals were produced and 19 J g(-1) of thermal energy absorbed) indicating that the 3D protein network was degraded. SDS-PAGE analysis indicated the breaking of intermolecular S-S bridges holding together the protein network rather than the primary structure of constituent proteins. Despite the relatively large concentration of OH radical produced in the sonication time range applied, no protein cross-linking was observed which can be attributed to the high degree of protein glycosylation and protein immobility. Differential scanning calorimetry (DSC) showed that both the amount of bound water and the enthalpy of denaturation of the constituent proteins are not affected by sonication, which is consistent with the SDS-PAGE results. A small increase in sample turbidity can be attributed to the small extent of thermal denaturation occurring in the vicinity of cavitation sites.

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The relations between absorbed dose, molecular mass distribution, and viscosity studied on aqueous solution of ovalbumin agglomerated by ? irradiation

Cited by 2 publications

References 11 publications

Macro- and Microscale Rheological Properties of Poly(vinyl alcohol) Aqueous Solutions

Macro- and Microscale Rheological Properties of Poly(vinyl alcohol) Aqueous Solutions

Sonolysis of High Macroviscosity Systems: Hen Albumen Hydrogel

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