The Relation of Urea to the Movement of Water in Liver Tissue

Opie, Eugene L.

doi:10.1073/pnas.46.4.477

Cited by 5 publications

(4 citation statements)

References 3 publications

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“…The quantity of amino compounds needed to produce skin necrosis has a diminishing sequence which follows the same order as molar concentrations of the same substances found to be isotonic with liver tissue in foregoing experiments (9 The relation of necrosis to isotonicity is significant because liver cells act as osmometers (1). The figures repeat the similar relation found between skin necrosis caused by electrolytes and molar concentration of solutions of corresponding substances isotonic with liver and with kidney.…”

Section: Effect Of Amino Acids and Related Substancessupporting

confidence: 65%

“…These experiment show that the quantity of different electrolytes necessary to produce necrosis of the skin vary with the valence of their basic ions, on the one hand, and, on the other, with the molar concentration of the corresponding electrolytes found in preceding experiments (9) to be in osmotic equilibrium with cells of the liver and of the kidney and in the same order.…”

Section: Effect Of Electrolytessupporting

confidence: 51%

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On the Relation of Necrosis and Inflammation to Denaturation of Proteins

Opie

1962

The Journal of Experimental Medicine

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112

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Necrosis of the skin was produced by the injection of measured quantities of electrolytes and of amino compounds into the dermis, and the relative ability of these substances to produce it was determined. Inflammation characterized by edema and accumulation of leucocytes accompanied necrosis. The ability of electrolytes to produce necrosis was found to increase with the valence of their basic ion, and in this respect was in accord with their ability to denature proteins. The quantity of different electrolytes needed to produce necrosis varied in the same order as the molar concentration of these electrolytes, that is isotonic with liver or kidney cells. Necrosis caused by amino compounds occurred with similar relation to the isotonicity of liver cells. In this as in other relations the cells acted as osmometers. The foregoing relations indicate that denaturation of proteins, necrosis of living tissue, and osmotic activity of liver or kidney cells are determined by molecular weight, valence, and ion-dissociation of electrolytes, that is, by the factors that determine the colligative properties of electrolytes. Agents such as turpentine, mustard, or croton oil and some halogen substitution compounds of methyl that are insoluble in water and soluble in lipoids have produced skin necrosis and inflammation.

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Section: Effect Of Amino Acids and Related Substancessupporting

confidence: 65%

Section: Effect Of Electrolytessupporting

confidence: 51%

On the Relation of Necrosis and Inflammation to Denaturation of Proteins

Opie

1962

The Journal of Experimental Medicine

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112

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“…Urea formed by the liver slices is presumably derived from amino acids, in part, at least, from arginine and related substances which like electrolytes are capable of maintaining osmotic pressure within liver cells (1). By oxidation urea is formed from them and being readily diffusible leaves the cells.…”

Section: Rec~itvlation and Discvssionmentioning

confidence: 99%

“…Slices of liver tissue have been immersed in Krebs-Ringer solution with all electrolytes 0.154 molar and with bicarbonate buffer. The method which has been used has been described in detail in preceding publications (1,2). Liver slices preferably weighing from 50 to 100 rag.…”

mentioning

confidence: 99%

The Relation of Oxygen Supply to Water Movement and to Urea Formation in Surviving Liver Tissue

Opie

1961

The Journal of Experimental Medicine

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In a preceding publication (1) evidence was presented to show that surviving liver cells in the presence of oxygen produce urea and lose water, whereas with anoxia little, if any, urea is formed and water enters the ceils in considerable quantity. I t seemed probable that intracellular amino acids and substances derived from them-for example, arginine, ornithine and citrulline-can maintain a high intraceilular osmotic pressure but with formation of urea, leave the ceils. Kidney cells which form little, if any, urea undergo swelling both in the presence and absence of oxygen.The purpose of the present study has been to determine how a varied supply of oxygen influences the movement of water in surviving fiver tissue. The a t t e m p t has been made to imitate in vitro as closely as possible conditions present during life.

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Die Beziehungen zwischen intracellul�rem osmotischen Druck und cellul�rem Wasserwechsel

Burck

1962

Klin Wochenschr

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The Relation of Urea to the Movement of Water in Liver Tissue

Cited by 5 publications

References 3 publications

On the Relation of Necrosis and Inflammation to Denaturation of Proteins

On the Relation of Necrosis and Inflammation to Denaturation of Proteins

The Relation of Oxygen Supply to Water Movement and to Urea Formation in Surviving Liver Tissue

Die Beziehungen zwischen intracellul�rem osmotischen Druck und cellul�rem Wasserwechsel

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