The Relation of Different-Scale Membrane Processes Under Nitric Oxide Influence

Brazhe, Nadezda A.; Erokhova, Liudmila; Чурин, А. А.; Максимов, Г. В.

doi:10.1007/s10867-005-2043-1

Cited by 9 publications

(1 citation statement)

References 41 publications

(38 reference statements)

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“…We have studied the intracellular dynamics in a broader frequency region and have shown that different types of neurons, erythrocytes and mast cells display regular changes in RI with frequencies in the 0.1-30 Hz range that are specific for each cell type (Sosnovtseva et al 2005;Brazhe et al 2005Brazhe et al , 2006. However, even if the best approaches to interference microscopy are applied, a significant amount of valuable information about the cell compartmentalization and dynamics may remain undetected.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive study of nerve fibres using laser interference microscopy

Brazhe

Rodionova

et al. 2008

Phil. Trans. R. Soc. A.

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This paper presents the results of a laser interference microscopy study of the morphology and dynamical properties of myelinated nerve fibres. We describe the principles of operation of the phase-modulated laser interference microscope and show how this novel technique allows us to obtain information non-invasively about the internal structure of different regions of a nerve fibre. We also analyse the temporal variations in the internal optical properties in order to detect the rhythmic activity in the nerve fibre at different time scales and to shed light on the underlying biological processes. We observe pronounced frequencies in the dynamics of the optical properties and suggest that the oscillatory modes have similar origin in different regions, but different strengths and mutual modulation properties.

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Section: Introductionmentioning

confidence: 99%

Non-invasive study of nerve fibres using laser interference microscopy

Brazhe

Rodionova

et al. 2008

Phil. Trans. R. Soc. A.

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Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry

Kopeikina

Dukhinova

Ponomarev

2018

Cellular Heterogeneity

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Neuronal cells are probably the less studied cells regarding their heterogeneity on a single cell or population levels. One of the main problems of studying of individual neurons is the presence of long processes (axons) on differentiated adult neurons that hamper their isolation without significant damage to the cells. Therefore, the most common method to study neuronal cells is immunofluorescent microscopy of sections of the brain, which remains poorly quantitative and allows analyzing a small number of fixed cells. Also, immunofluorescent microscopy has a number of staining artifacts since histology section has high level of autofluorescence and non-specific binding of fluorescent probes. Alternative methods that could overcome disadvantages of immunofluorescent histology include flow cytometry, scanning cytometry, and laser interferometry. Flow cytometry and, to some extent of degree, scanning cytometry allow performing analysis of multiple markers with a low level of non-specific background and very robust statistics. Laser interferometry allows studies intact, alive neurons without staining. Limitations and advantages of these methods are discussed in this chapter.

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Neuroplastic and neuropathological changes in the central nervous system of the Gray mussel Crenomytilus grayanus (Dunker) under environmental stress

Kotsyuba

Vaschenko

2010

Invert Neurosci

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We studied here neuron ultrastructure, synaptic plasticity and subcellular localization of NADPH-diaphorase (NADPH-d), a cytochemical marker for nitric oxide syntase, in the pedal ganglia of the Gray mussel Crenomytilus grayanus sampled from the polluted and reference sites in Amursky Bay (Sea of Japan) at lower and higher water temperature (in the beginning and the end of August, respectively). At lower temperature, neuroplastic changes in mussel ganglia prevailed: a sharp increase in the number of cytosomes in NADPH-d-positive neurons and a sharp decrease in the number of mitochondria in both NADPH-d-positive and NADPH-d-negative neurons. At higher temperature, neurodegenerative changes prevailed: disruption of a part of NADPH-d-negative axons and interneuronal contacts, formation of concentric lamellar structures in the neuropils, and accumulation of autophagosomes in NADPH-d negative neurons. The results suggest that the stress-induced production of nitric oxide in cytosomes of mussel neurons and plasticity of gap junctions have a neuroprotective effect.

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The Relation of Different-Scale Membrane Processes Under Nitric Oxide Influence

Cited by 9 publications

References 41 publications

Non-invasive study of nerve fibres using laser interference microscopy

Non-invasive study of nerve fibres using laser interference microscopy

Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry

Neuroplastic and neuropathological changes in the central nervous system of the Gray mussel Crenomytilus grayanus (Dunker) under environmental stress

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