The relation between recalcitrancy of a mangrove plant, Kandelia obovata, and high endogenous level of abscisic acid

Kaai, Fumiki; Kawana, Yoshifumi; Sasamoto, Hamako

doi:10.1007/s11240-008-9394-9

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…Recalcitrancy might be due to the production of free radicals, lipid peroxidases, and toxic, aldehydic lipid peroxidation products (Benson 2000) levels of these compounds vary in response to different tissue culture manipulations. Kaai et al (2008) found a close relation between the recalcitrancy and the abscisic acid. However, recalcitrancy was found when plants were subcultured in vitro.…”

Section: Resultsmentioning

confidence: 84%

Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L.

Murthy¹,

Kondamudi²

2012

Plant Tissue Cult. & Biotech.

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The thin cell layers of nodes and internodes of Ceropegia spiralis L. were cultured on MS supplemented with BAP 13.32 µM/l + NAA 0.537 μM/l induced 17.34 ± 0.55 shoots showing extensive growth. Later on the organogenesis was also induced on MS containing BAP 13.32 µM/l + 2, 4-D 1.130 µM/l, whereas the medium with BAP 13.32 µM/l + 2, 4-D 4.52 µM/l has the highest callus producing ability in recalcitrants as well as in normal explants. Shoots developed were rooted best on 0.5 MS with NAA 10.74 μM/l. Optimum shoot and root multiplication was obtained within eight weeks. In vitro plantlets were successfully weaned and transferred to soil with about 90 per cent survival rate. So far, more than 650 weanlings have been produced successfully and reintroduced into nature for their recovery. Key words: Ceropegia spiralis, Thin cell layer explants, Recalcitrants, Regeneration D. O. I. 10.3329/ptcb.v21i1.9564 Plant Tissue Cult. & Biotech. 21(1): 63-73, 2011 (June)

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Section: Resultsmentioning

confidence: 84%

Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L.

Murthy¹,

Kondamudi²

2012

Plant Tissue Cult. & Biotech.

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“…For example, growth inhibition of cotyledon-origin suspension cells of A. alba by the addition of ABA in the protoplast culture medium was caused by the endogenous levels of cis-ABA (3.7 to 43 pmoles / 6 × 10 6 protoplasts, Hasegawa et al, 2011). In another mangrove plant, Kandelia obovata, high content of cis-ABA in leaf protoplasts was reported for the cause of recalcitrancy of callus growth (Kaai et al, 2008).…”

Section: Carotenoid As a Putative Allelochemical In Yellow Callus Of mentioning

confidence: 99%

High Allelopathic Activity of Carotenoid-accumulating Callus of a Halophilic Mangrove Plant, Avicennia alba: Protoplast Co-culture Method with Digital Image Analysis

Sasamoto¹,

Hayatsu²,

Suzuki³

2019

JPS

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A yellow callus strain was established from hypocotyls of a halophilic mangrove plant, Avicennia alba, by subculture in the dark. Allelopathic activities of yellow A. alba callus were assayed using recipient lettuce protoplasts at three growth stages by the protoplast co-culture method with digital image analysis. The protoplast cultures of yellow A. alba callus were halophilic to NaCl, KCl, and MgCl2 (up to 200 mM) but not to CaCl2. By contrast, NaCl and KCl inhibited the growth of non-salt-tolerant lettuce protoplasts, while CaCl2 and MgCl2 stimulated their growth at low concentrations. Highly salt-tolerant or halophilic mangrove plant cells were expected to have low allelopathic activity, but the protoplasts of yellow A. alba callus had very strong allelopathic activity. The inhibition was strongest at the cell division stage with growth being inhibited to 50% and 9% of the control by 104 mL-1 and 5 × 104 mL-1 of A. alba, respectively. There was less inhibition at the yellow pigment accumulation stage of lettuce. Stimulation was observed at the early cell wall formation stage with up to 105 mL-1 of A. alba. The yellow pigment of yellow A. alba callus was extracted with hexane and its absorption spectrum showed the wavelength peaks of a carotenoid, neoxanthin. Using transmission electron microscopy, specific electron-dense structures were found in yellow A. alba callus, which were similar to the undeveloped ultrastructure of a carotenoid. A carotenoid was strongly suggested to be the putative allelochemical in yellow callus of A. alba.

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“…Rhizospheric bacteria are living in the direct vicinity of the roots, and root-associated bacteria play important roles in mangrove ecosystem involving nitrogen fixation (Alfaro-Espinoza and Ullrich, 2015), nutrient acquisition (Holguin et al, 2001; Berendsen et al, 2012; Prakash et al, 2015), abiotic stress tolerance (Rout and Southworth, 2013), as well as production of regulators of plant growth and development such as auxins, cytokinins, and gibberellins (Kaai et al, 2008). Additionally, the microbial communities associated with root also play an essential role in the matter cycle (for instance phosphorus, organic acids, and siderophores) and maintenance of the health of wetland ecosystem (Gomes et al, 2011; Zeng et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Diverse Profiles of AI-1 Type Quorum Sensing Molecules in Cultivable Bacteria from the Mangrove (Kandelia obovata) Rhizosphere Environment

Lao

Jin

et al. 2016

Front. Microbiol.

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Mangrove rhizosphere environment harbors diverse populations of microbes, and some evidence showed that rhizobacteria behavior was regulated by quorum sensing (QS). Investigating the diverse profiles of QS molecules in mangrove ecosystems may shed light on the bacterial roles and lead to a better understanding of the symbiotic interactions between plants and microbes. The aims of the current study focus on identifying AI-1 type QS signals, i.e., acyl homoserine lactones (AHLs), in Kandelia obovata rhizosphere environment. Approximately 1200 rhizobacteria were screened and 184 strains (15.3%) tested were positive. Subsequent 16s rRNA gene sequencing and dereplication analyses identified 24 species from the positive isolates, which were affiliated to three different phyla, including Proteobacteria, Firmicutes, and Actinobacteria. Thin-layer chromatography separation of extracts revealed diverse AHL profiles and detected at least one active compound in the supernatant of these 24 cultivable AHL-producers. The active extracts from these bacterial isolates were further evaluated by ultra performance liquid chromatography-mass spectrometry, and the carbon side chain length ranged from C4 to C14. This is the first report on the diversity of AI-1 type auto-inducers in the mangrove plant K. obovata, and it is imperative to expand our knowledge of plant-bacteria interactions with respect to the maintenance of wetland ecosystem health.

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The relation between recalcitrancy of a mangrove plant, Kandelia obovata, and high endogenous level of abscisic acid

Cited by 8 publications

References 16 publications

Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L.

Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L.

High Allelopathic Activity of Carotenoid-accumulating Callus of a Halophilic Mangrove Plant, Avicennia alba: Protoplast Co-culture Method with Digital Image Analysis

Diverse Profiles of AI-1 Type Quorum Sensing Molecules in Cultivable Bacteria from the Mangrove (Kandelia obovata) Rhizosphere Environment

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