The Relation between Biochemical Parameters, Milk Amyloid A, Somatic Cell Count, and Some Pathogens in Buffalo Milks

Abstract

Being resistant to hard environmental conditions and diseases makes Buffalo to have its valuable milk. Microbial contamination may occur due to undesirable conditions such as mastitis, environmental contamination, and stress. If microorganisms are not removed from the milk, it causes many production disadvantages including inadequacy of production, failure of fermentation and shortening of the shelf life. This study was conducted to determine the relationship between somatic cells count (SCC), the presence of some pathogens, and milk amyloid A (MAA) in the buffalo milk. In addition, oxidative stress in buffalo milk was evaluated. For this purpose, 70 samples were collected and Enterobacteriaceae, coliform microorganisms, Escherichia coli, Salmonella spp. analyses were performed. Biochemical parameters [Malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), catalase, superoxide dismutase (SOD), and total antioxidant capacity (TAC)] and serological parameter (MAA) were measured. The SCC was not statistically different according to Enterobacteriaceae, coliform microorganisms, E. coli groups. While MDA, NO, SOD, and TAC values were not statistically different according to the SCC groups, GSH and catalase values were statistically different. MAA values were statistically significant compared to the SCC groups. Moreover, there was a positive correlation between MAA and MDA/SCC. Detection of MAA may prevent the mixing of healthy and mastitic milk. Therefore, more reliable buffalo milk products will be presented to consumption.