The regulatory role of external cations on human polymorphonuclear phagocyte chemiluminescence

Abstract

SUMMARYThe effect of the external cations Na + and Ca 2+ on polymorphonuclear chemiluminescence was investigated. Both Ca 2+ in the range of 0.2-2 mM and Na + in the range of 114-I43 mM showed a dose dependent increase in polymorphonuclear chemiluminescence, irrespective of the concurrent increase in osmolality. The Na+/H + antiport inhibitor Amiloride decreased the response significantly. These effects were observed using buffers commonly used for chemiluminescence studies and indicate the importance of defming the Ca 2+ and Na + composition of the buffers used in chemiluminescence assays. 0920-8534/90/$03.50