The regulatory effect of veratric acid on NO production in LPS-stimulated RAW264.7 macrophage cells

Choi, Won Chul; Shin, Pyung-Gyun; Lee, Jong‐Hwan; Kim, Gun‐Do

doi:10.1016/j.cellimm.2012.12.007

Cited by 94 publications

(42 citation statements)

References 38 publications

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“…The 21 chemical constituents shown in Table 1 were identified from full composition granules of XFHM. Several lines of evidence indicate that Veratric acid [33], Loganic acid [34], Paeoniflorin [35], Loganin [36] and apiin [37] have anti-inflammatory and immune-regulatory activities. Gentiopicroside [38], Astragaloside IV [39], Acteoside [40] and Prime-O-glucosylcimifugin [41] exert anti-inflammatory effects by inhibiting the activation of NF-κB and other signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Li¹,

Wei²,

Li³

et al. 2017

BMC Complement Altern Med

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BackgroundXian-Fang-Huo-Ming-Yin (XFHM), a traditional herbal formula, has been used to treat sores and carbuncles for hundreds of years in Asia. Nowadays, its clinical effects in treatment of rheumatoid arthritis (RA) have been validated. In this study, we want to study its possible molecular mechanisms of regulating the differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis (CIA) mice for RA treatment.MethodsA high performance liquid chromatography-electrospray ionization/mass spectrometer (HPLC-ESI/MSn) system was used to analyze the constituents of XFHM granules. An arthritics mouse model was induced by collagen and leflunomide (LEF) was used as a positive control medicine. Pathological changes at the metatarsophalangeal joint were studied through Safranin O and immunohistochemical staining. The differentiation of T, B and NK cells was examined by flow cytometry and pro-inflammatory cytokines were assayed using an Inflammation Antibody Array assay. The expression of key molecules of the nuclear factor κB (NF-κB) and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathways in spleen were studied by western-blot analysis.ResultsIn our study. 21 different dominant chemical constituents were identified in XFHM. Treatment with XFHM suppressed the pathological changes in arthrosis of CIA. Additionally, XFHM down-regulated the proliferation and differentiation of CD3+ T cells and CD3−CD19+ B cells significantly. However, XFHM had no significant effect on CD3−NK1.1+ NK cells. Further study showed that the production of pro-inflammatory cytokines had been suppressed by inhibiting the activation of NF-κB and JAK/STAT signaling.ConclusionsXFHM can regulate and maintain the immunologic balance of lymphocytic immunity and inhibit the production of pro-inflammatory cytokines, thus suppressing the pathological changes of RA. Therefore, XFHM may be used as an application of traditional medicine against RA in modern complementary and alternative therapeutics.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1526-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Li¹,

Wei²,

Li³

et al. 2017

BMC Complement Altern Med

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“…Cell viability was examined to use WST‐1 solution (Daeil Lab Service, Seoul, Korea), modified MTT assay [13]. RAW264.7 and HEK293 cells were seeded in 96‐well plates at 1 × 10 5 cells/well.…”

Section: Methodsmentioning

confidence: 99%

Toluhydroquinone from A spergillus sp. suppress inflammatory mediators via nuclear factor-κB and mitogen-activated protein kinases pathways in lipopolysaccharide-induced RAW264.7 cells

Hwang

Son

Shin

et al. 2015

Journal of Pharmacy and Pharmacology

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“…The MAPK family plays important roles in LPS-induced pro-inflammatory cytokine production in many cell types [34]. In cells stimulated by LPS, the phosphorylation of MAPK is involved in the activation of transcription factors including AP-1, NF-κB, and subsequently produced cytokines [35,36]. Some reports revealed that pro-inflammatory mediators are regulated by the down-regulation of MAPK and NF-κB in LPS treated RAW 264.7 cells [37,38].…”

Section: Discussionmentioning

confidence: 99%

2-Phenylnaphthalene Derivatives Inhibit Lipopolysaccharide-Induced Pro-Inflammatory Mediators by Downregulating of MAPK/NF-κB Pathways in RAW 264.7 Macrophage Cells

Chang¹,

Liao²,

Ch³

2017

PLoS ONE

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The anti-inflammatory pharmacological effect of eight 2-phenylnaphthalenes (PNAP-1−PNAP-8) on lipopolysaccharide (LPS)-induced RAW 264.7 (a mouse cell line) was investigated. Among them, 6,7-dihydroxy-2-(4′-hydroxyphenyl)naphthalene (PNAP-6) and 2-(4′-aminophenyl)-6,7-dimethoxynaphthalene (PNAP-8) exhibited the best anti-inflammatory activity in this study. PNAP-6 and PNAP-8 not only significantly decreased the expression of inducible nitric oxide synthase and cyclooxygenase-II, but also inhibited the production of nitric oxide, interleukin-6, and tumor necrosis factor-α in LPS stimulated cells. Moreover, PNAP-6 and PNAP-8 inhibited nuclear factor (NF)-κB activation by decreasing the degradation of IκB and nuclear translocation of NF-κB subunit (p65). In addition, PNAP-6 and PNAP-8 also attenuated the phosphorylation of ERK, p38, and JNK. These results suggest that PNAP-6 and PNAP-8 exert anti-inflammatory activities by down regulating NF-κB activation and the mitogen-activated protein kinase signaling pathway in LPS-stimulated Raw 264.7 cells. This is the first study demonstrating that PNAPs can inhibit LPS-induced pro-inflammatory mediators in macrophages cells.

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The regulatory effect of veratric acid on NO production in LPS-stimulated RAW264.7 macrophage cells

Cited by 94 publications

References 38 publications

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice

Toluhydroquinone from A spergillus sp. suppress inflammatory mediators via nuclear factor-κB and mitogen-activated protein kinases pathways in lipopolysaccharide-induced RAW264.7 cells

2-Phenylnaphthalene Derivatives Inhibit Lipopolysaccharide-Induced Pro-Inflammatory Mediators by Downregulating of MAPK/NF-κB Pathways in RAW 264.7 Macrophage Cells

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