The regulatory effect of Lactobacillus rhamnosus GG on T lymphocyte and the development of intestinal villi in piglets of different periods

Abstract

The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control group (saline group). The LGG group piglets were given 1 ml LGG (6 × 10 9 CFU/ml) per day. The saline group was given 1 ml of normal saline per day. The piglets were slaughtered at 30 days and 45 days, respectively, and the MLN, jejunum and ileum PPs, LP of the piglets were taken. The expression of CD3 + CD4 + T lymphocytes was detected by flow cytometry, and intestinal villi development was observed by intestinal paraffin section. The results showed that the flow cytometry results at 30 days and 45 days showed that the CD3 + CD4 + T lymphocytes in the MLN group were significantly different from those in the saline group (P < 0.05, P < 0.01).The CD3 + CD4 + T lymphocytes in the jejunum PP of piglets in LGG group were significantly different from those in saline group (P < 0.05). The CD3 + CD4 + T lymphocytes in the ileum PP of the LGG group were significantly different from those in the saline group (P < 0.05, P < 0.01). CD3 + CD4 + T lymphocytes and normal saline in the piglets of the LGG group There was a significant difference between the two groups (P < 0.001, P < 0.05). P < 0.001). HE staining results showed the length of the LGG group ileal villi in piglets at 30 days, 45 days was significantly different from that in normal saline group (P < 0.01, P < 0.01). LGG can also regulate the proliferation of T lymphocytes in the intestine of early weaned piglets at 30 days and 45 days increase the number of CD3 + CD4 + T lymphocytes.

The Role of Probiotics in Alleviating Postweaning Diarrhea in Piglets From the Perspective of Intestinal Barriers

“…Van Baarlen et al., ( van Baarlen et al., 2011 ) reported that lipoteichoic acid, a cell surface molecule of Lactobacillus plantarum, regulated the activation of extracellular signaling kinase through the TLR signaling pathway, in turn activating NF-κB to regulate the release of Th1 cytokines and subsequent T reg and Th1 development. Lactobacillus rhamnosus can regulate the proliferation of T-lymphocytes and increase the number of CD3+ CD4+ T-lymphocytes in the intestine of early weaning piglets ( Shonyela et al., 2020 ). In addition, probiotics also play a role in the stimulation of antibodies in the gut, particularly slgA, which can inhibit pathogen adherence to IECs.…”

Probiotic Lactobacillus rhamnosus GG Promotes Mouse Gut Microbiota Diversity and T Cell Differentiation

“…Our recent study also found that LGG can protect the development and integrity of intestinal villi, maintain the integrity of intestinal villi, and promote the growth of villi length. LGG can also regulate the proliferation of T-lymphocytes in the intestine of early weaning piglets at 30 days and 45 days, and increase the number of CD3+ CD4+ T-lymphocytes ( Shonyela et al, 2020 ). Colonization of germ-free mice with either Lactobacillus johnsonii (NCC 533) or Lactobacillus paracasei (NCC 2461) induced similar germinal center formation and immunoglobulin A-bearing lymphocytes in the mucosa, suggesting that LAB can activate mucosal B-cell responses ( Ibnou-Zekri et al, 2003 ).…”

Comparison between Lactobacillus rhamnosus GG and LuxS-deficient strain in regulating gut barrier function and inflammation in early-weaned piglets

“…The study confirmed that daily gain and starter conversion had a remarkable increased after feeding piglets with Lactobacillus johnsonii BS15, which was mostly ascribe to improved gut development and digestion by ileal villus height and crypt depth ( 38 ). Studies also have confirmed that the integrity of the jejunum and ileum villi in piglets at age of 25 days after weaning was better than the control group at 30 and 45 days of age, indicating that LGG effectively protect the normal development of intestinal villi ( 39 ). In this study, we found that compared with ΔluxS, WT could better alleviate intestinal damage caused by weaning.…”