The regulator CdsS/CdsR two-component system modulates expression of genes involved in chitin degradation of Pseudoalteromonas piscicida strain O-7

Miyamoto, Katsushiro; Okunishi, Mina; Nukui, Eiji; Kobayashi, Takeshi; Imada, Chiaki; Tsujibo, Hiroshi

doi:10.1007/s00203-007-0283-0

Cited by 9 publications

(6 citation statements)

References 45 publications

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“…Additionally, at least three extracellular polysaccharide deacetylases (COG0726) allow P. tunicata to release acetyl units from polysaccharides such as chitin, and two of these deacetylases had no homologs in any other Alteromonadales genome. Furthermore, chitin degradation might be controlled by homologs of the CdsS/CdsR two-component regulator system, which in Pseudoalteromonas piscicida strain O-7 was characterized to modulate the expression of chitin degradation [78], [79].…”

Section: Resultsmentioning

confidence: 99%

Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment

et al. 2008

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BackgroundColonisation of sessile eukaryotic host surfaces (e.g. invertebrates and seaweeds) by bacteria is common in the marine environment and is expected to create significant inter-species competition and other interactions. The bacterium Pseudoalteromonas tunicata is a successful competitor on marine surfaces owing primarily to its ability to produce a number of inhibitory molecules. As such P. tunicata has become a model organism for the studies into processes of surface colonisation and eukaryotic host-bacteria interactions.Methodology/Principal FindingsTo gain a broader understanding into the adaptation to a surface-associated life-style, we have sequenced and analysed the genome of P. tunicata and compared it to the genomes of closely related strains. We found that the P. tunicata genome contains several genes and gene clusters that are involved in the production of inhibitory compounds against surface competitors and secondary colonisers. Features of P. tunicata's oxidative stress response, iron scavenging and nutrient acquisition show that the organism is well adapted to high-density communities on surfaces. Variation of the P. tunicata genome is suggested by several landmarks of genetic rearrangements and mobile genetic elements (e.g. transposons, CRISPRs, phage). Surface attachment is likely to be mediated by curli, novel pili, a number of extracellular polymers and potentially other unexpected cell surface proteins. The P. tunicata genome also shows a utilisation pattern of extracellular polymers that would avoid a degradation of its recognised hosts, while potentially causing detrimental effects on other host types. In addition, the prevalence of recognised virulence genes suggests that P. tunicata has the potential for pathogenic interactions.Conclusions/SignificanceThe genome analysis has revealed several physiological features that would provide P. tunciata with competitive advantage against other members of the surface-associated community. We have also identified properties that could mediate interactions with surfaces other than its currently recognised hosts. This together with the detection of known virulence genes leads to the hypothesis that P. tunicata maintains a carefully regulated balance between beneficial and detrimental interactions with a range of host surfaces.

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Section: Resultsmentioning

confidence: 99%

Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment

et al. 2008

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“…This TCS is also involved in surface colonization, biofilm formation, or microbial dispersion from biofilms in some marine bacteria (330,331,369). In marine Pseudoalteromonas piscicida isolates, the CdsS/CdsR TCS regulates the expression of genes involved in chitin degradation (370), which is facilitated by bacterial surface colonization. In V. cholerae, the expression of chitin-inducible genes, including those involved in chitin degradation and utilization, chemotaxis, surface colonization, and natural competence, is modulated by the orphan TCS sensor kinase ChiS (371,372).…”

Section: Microbial Two-component Signal Transduction Systemsmentioning

confidence: 99%

Microbial Surface Colonization and Biofilm Development in Marine Environments

Dang

Lovell

2016

Microbiol Mol Biol Rev

846

636

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SUMMARYBiotic and abiotic surfaces in marine waters are rapidly colonized by microorganisms. Surface colonization and subsequent biofilm formation and development provide numerous advantages to these organisms and support critical ecological and biogeochemical functions in the changing marine environment. Microbial surface association also contributes to deleterious effects such as biofouling, biocorrosion, and the persistence and transmission of harmful or pathogenic microorganisms and their genetic determinants. The processes and mechanisms of colonization as well as key players among the surface-associated microbiota have been studied for several decades. Accumulating evidence indicates that specific cell-surface, cell-cell, and interpopulation interactions shape the composition, structure, spatiotemporal dynamics, and functions of surface-associated microbial communities. Several key microbial processes and mechanisms, including (i) surface, population, and community sensing and signaling, (ii) intraspecies and interspecies communication and interaction, and (iii) the regulatory balance between cooperation and competition, have been identified as critical for the microbial surface association lifestyle. In this review, recent progress in the study of marine microbial surface colonization and biofilm development is synthesized and discussed. Major gaps in our knowledge remain. We pose questions for targeted investigation of surface-specific community-level microbial features, answers to which would advance our understanding of surface-associated microbial community ecology and the biogeochemical functions of these communities at levels from molecular mechanistic details through systems biological integration.

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“…Chitinolytic activity was observed in bacteria cultured on media containing colloidal or glycol chitin, N -acetylglucosamine, chitooligosaccarides, or the cell walls of certain fungi. No or minimal activity was observed in the same bacteria cultured on media containing glucose or laminarin as the source of carbon [69, 88, 124]. Molecular studies have detected the presence of a two-component signal transduction system that regulates the synthesis of CHIs in Pseudoalteromonas piscicida O-7, Streptomyces thermoviolaceus OPC-520, and other bacteria.…”

Section: Chitinolytic Enzymesmentioning

confidence: 99%

Chitinolytic Microorganisms and Their Possible Application in Environmental Protection

et al. 2013

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This paper provides a review of the latest research findings on the applications of microbial chitinases to biological control. Microorganisms producing these enzymes can inhibit the growth of many fungal diseases that pose a serious threat to global crop production. Currently, efforts are being made to discover producers of chitinolytic enzymes. The potential exists that natural biofungicides will replace chemical fungicides or will be used to supplement currently used fungicides, which would reduce the negative impact of chemicals on the environment and support the sustainable development of agriculture and forestry.

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The regulator CdsS/CdsR two-component system modulates expression of genes involved in chitin degradation of Pseudoalteromonas piscicida strain O-7

Cited by 9 publications

References 45 publications

Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment

Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment

Microbial Surface Colonization and Biofilm Development in Marine Environments

Chitinolytic Microorganisms and Their Possible Application in Environmental Protection

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