The regulation of vesicle trafficking by small GTPases and phospholipids during pollen tube growth

Zhang, Yan; McCormick, Sheila

doi:10.1007/s00497-009-0118-z

Cited by 39 publications

(27 citation statements)

References 57 publications

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“…The tip bulging phenotype observed was subtle relative to previously reported phenotypes associated with loss of polarity (e.g. Li et al , 1999; Dowd et al , 2006; Yoon et al , 2006) and is more reminiscent of that associated with perturbing vesicle trafficking by overexpressing Rab11b GTPase (de Graaf et al , 2005; Zhang and McCormick, 2010). As a result, it is speculated that the disturbance in morphology is related more to effects on the rate of extension or possibly osmoregulation than the regulation of polarity per se .…”

Section: Resultscontrasting

confidence: 63%

The pollen-specific R-SNARE/longin PiVAMP726 mediates fusion of endo- and exocytic compartments in pollen tube tip growth

Guo

McCubbin

2012

Journal of Experimental Botany

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The growing pollen tube apex is dedicated to balancing exo- and endocytic processes to form a rapidly extending tube. As perturbation of either tends to cause a morphological phenotype, this system provides tractable model for studying these processes. Vesicle-associated membrane protein 7s (VAMP7s) are members of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family that mediate cognate membrane fusion but their role in pollen tube growth has not been investigated. This manuscript identifies PiVAMP726 of Petunia inflata as a pollen-specific VAMP7 that localizes to the inverted cone of transport vesicles at the pollen tube tip. The endocytic marker FM4-64 was found to colocalize with yellow fluorescent protein (YFP)-PiVAMP726, which is consistent with PiVAMP726 containing an amino-acid motif implicated in endosomal localization, At high overexpression levels, YFP- PiVAMP726 inhibited growth and caused the formation of novel membrane compartments within the pollen tube tip. Functional dissection of PiVAMP726 implicated the N-terminal longin domain in negative regulation of the SNARE activity, but not localization of PiVAMP726. Expression of the constitutively active C-terminal SNARE domain alone, in pollen tubes, generated similar phenotypes to the full-length protein, but the truncated domain was more potent than the wild-type protein at both inhibiting growth and forming the novel membrane compartments. Both endo- and exocytic markers localized to these compartments in addition to YFP-PiVAMP726, leading to the speculation that PiVAMP726 might be involved in the recycling of endocytic vesicles in tip growth.

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Section: Resultscontrasting

confidence: 63%

The pollen-specific R-SNARE/longin PiVAMP726 mediates fusion of endo- and exocytic compartments in pollen tube tip growth

Guo

McCubbin

2012

Journal of Experimental Botany

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“…The function of stigmatic RLCKs in compatible pollination is still unknown. The third category consists of genes encoding leucine-rich repeat RLKs that are involved in regulation of pollen tube growth in petunia and tomato [36-38]. Examples in this category include LePRK1 and LePRK2, which are two well-defined pollen kinases that act in pollen-pistil interaction primarily by perceiving signals from pistil to regulate pollen tube growth.…”

Section: Resultsmentioning

confidence: 99%

Identification of genes specifically or preferentially expressed in maize silk reveals similarity and diversity in transcript abundance of different dry stigmas

Chen

Sang

et al. 2012

BMC Genomics

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BackgroundIn plants, pollination is a critical step in reproduction. During pollination, constant communication between male pollen and the female stigma is required for pollen adhesion, germination, and tube growth. The detailed mechanisms of stigma-mediated reproductive processes, however, remain largely unknown. Maize (Zea mays L.), one of the world’s most important crops, has been extensively used as a model species to study molecular mechanisms of pollen and stigma interaction. A comprehensive analysis of maize silk transcriptome may provide valuable information for investigating stigma functionality. A comparative analysis of expression profiles between maize silk and dry stigmas of other species might reveal conserved and diverse mechanisms that underlie stigma-mediated reproductive processes in various plant species.ResultsTranscript abundance profiles of mature silk, mature pollen, mature ovary, and seedling were investigated using RNA-seq. By comparing the transcriptomes of these tissues, we identified 1,427 genes specifically or preferentially expressed in maize silk. Bioinformatic analyses of these genes revealed many genes with known functions in plant reproduction as well as novel candidate genes that encode amino acid transporters, peptide and oligopeptide transporters, and cysteine-rich receptor-like kinases. In addition, comparison of gene sets specifically or preferentially expressed in stigmas of maize, rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana [L.] Heynh.) identified a number of homologous genes involved either in pollen adhesion, hydration, and germination or in initial growth and penetration of pollen tubes into the stigma surface. The comparison also indicated that maize shares a more similar profile and larger number of conserved genes with rice than with Arabidopsis, and that amino acid and lipid transport-related genes are distinctively overrepresented in maize.ConclusionsMany of the novel genes uncovered in this study are potentially involved in stigma-mediated reproductive processes, including genes encoding amino acid transporters, peptide and oligopeptide transporters, and cysteine-rich receptor-like kinases. The data also suggest that dry stigmas share similar mechanisms at early stages of pollen-stigma interaction. Compared with Arabidopsis, maize and rice appear to have more conserved functional mechanisms. Genes involved in amino acid and lipid transport may be responsible for mechanisms in the reproductive process that are unique to maize silk.

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“…The process of pollen tube growth involves extensive vesicle trafficking and actin cytoskeleton rearrangements in the pollen tube, and a number of pollen factors involved in the regulation of these processes have been identified including Rop GTPases (reviewed in Yang 2008;Yalovsky et al 2008;Zhang and McCormick 2010;Zonia 2010). GEFs are responsible for stimulating small GTPases to exchange GDP to GTP, and thus, switch from an inactive to an active form of the GTPase.…”

Section: The S Receptor Kinase In Early Pollen-stigma Interactions Inmentioning

confidence: 99%

The Regulation of Pollen–Pistil Interactions by Receptor-Like Kinases

Indriolo

Goring

2011

Signaling and Communication in Plants

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Receptor-Like Kinases (RLKs) have been discovered to regulate several signaling events during pollen-pistil interactions, with functions uncovered in both the pollen tube and the pistil. The earliest role is for S Receptor Kinase (SRK) in the rapid activation of the Brassicaceae self-incompatible response in the stigma following the perception of self-pollen. The next role is in the communication of a growing pollen tube with the transmitting tract of the Solanum lycopersicum pistils through the Pollen Receptor Kinases (LePRKs). Once the pollen tube has reached the ovule, it is fundamentally important for the pollen tube to have the correct timing to cease growth and release the sperm cells for the successful double fertilization of the egg cell and central cell. In Arabidopsis thaliana, this event is mediated by RLKs on both the female (FERONIA/SIRENE) and male (ANXUR1 and ANXUR2) side. Thus, RLKs play several fundamental roles in the successful creation of the next generation. This chapter will review the functions of these receptor kinases as well as other players that work alongside with these RLKs in regulating these precise steps during pollen-pistil interactions.

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The regulation of vesicle trafficking by small GTPases and phospholipids during pollen tube growth

Cited by 39 publications

References 57 publications

The pollen-specific R-SNARE/longin PiVAMP726 mediates fusion of endo- and exocytic compartments in pollen tube tip growth

The pollen-specific R-SNARE/longin PiVAMP726 mediates fusion of endo- and exocytic compartments in pollen tube tip growth

Identification of genes specifically or preferentially expressed in maize silk reveals similarity and diversity in transcript abundance of different dry stigmas

The Regulation of Pollen–Pistil Interactions by Receptor-Like Kinases

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