The regulation of the Oct-1 gene transcription is mediated by two promoters

Pankratova, E. V.; Sytina, E. V.; Luchina, N N; Krivega, Ivan

doi:10.1016/s0165-2478(03)00026-9

Cited by 15 publications

(7 citation statements)

References 29 publications

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“…However, over the years Prankratova and associates have determined, using mouse Oct-1 promoter, that there are actually multiple promoters for expressing the Oct-1 coding sequences. An up-stream transcriptional promoter appears rich in Sp1 motifs and has a ubiquitous and constitutive expression activity [33,34]. This finding is consistent with many others that Sp1 is a housekeeping transcription factor [35].…”

Section: Transcriptional Up-regulation Of the Oct-1 Gene By Ox-ldlsupporting

confidence: 89%

LOX-1 Transcription

Hermonat

Zhu

Cao

et al. 2011

Cardiovasc Drugs Ther

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The importance of the lectin-like oxidized LDL receptor (LOX-1) gene in cardiovascular and other diseases is slowly being revealed. LOX-1 gene expression appears to be a "canary in a coal mine" for atherogenesis, being strongly up-regulated early on in a number of cell types when they are activated, and predicting the sites of future disease. From this early time point the LOX-1 protein often participates in the disease process itself. While gene/protein expression can be regulated on a multiplicity of levels, the most basic and important mode of regulation is usually transcriptional. There are very few studies on the transcriptional regulation of the human LOX-1 promoter; fewer still on definitive mapping of the transcription factors involved. It is known that a wide variety of stimuli up-regulate LOX-1, usually/probably on the transcriptional level. Angiotensin II (Ang II) is one important regulator of renin-angiotensin system and stimulator LOX-1. Ang II is known to up-regulate LOX-1 transcription through an NF-kB motif located at nt -2158. Oxidized low density lipoprotein (ox-LDL) is another important cardiovascular regulator, particularly of atherosclerotic disease, and a strong stimulator of LOX-1. Ox-LDL is known to up-regulate LOX-1 transcription through an Oct-1 motif located at nt -1556. The subsequent enhanced LOX-1 receptor numbers and their binding by ox-LDL ligand triggers a positive feedback loop, increasing further LOX-1 expression, with a presently unknown regulatory governor. The Oct-1 gene also has its own Oct-1-driven positive feedback loop, which likely also contributes to LOX-1 up-regulation. There is also data which suggests the involvement of the transcription factor AP-1 during stimulation with Phorbol 12-myristate acetate. While the importance of NF-κB as a transcriptional regulator of cardiovascular-relevant genes is well known, the importance of Oct-1 is not. Data suggests that Oct-1-mediated up-regulation of transcription is an early event in the stimulation of LOX-1 by ox-LDL. Yet Oct-1 also down-regulates cardiovascular-relevant genes by suppressing NF-κB transactivation. Thus, Oct-1 is presently somewhat of an enigma, up-regulating and down-regulating genes seemingly at random without an overall theme (with the exception of cell cycle). Yet the up-regulation of LOX-1 by ox-LDL is a very important event in atherogenesis (both early and late) and Oct-1 is, therefore, an important transcriptional gatekeeper of this important atherogenic trigger.

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Section: Transcriptional Up-regulation Of the Oct-1 Gene By Ox-ldlsupporting

confidence: 89%

LOX-1 Transcription

Hermonat

Zhu

Cao

et al. 2011

Cardiovasc Drugs Ther

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“…Such isoform-specific regulation has been reported for a variety of genes, including OCT1 (Pankratova et al, 2003), AFP (Scohy et al, 2000), CATL (Seth et al, 2003), and BRCA1 (Wardrop and Brown, 2005). Advances in bioinformatics have provided a prediction algorithm for the identification of highly conserved common regulatory motifs in established human promoters (Xie et al, 2005).…”

mentioning

confidence: 94%

OCT4B expression in PBMNCs suggests the existence of an alternative OCT4 promoter

Papamichos

Lambropoulos

Kotoula

2009

Genes Chromosomes & Cancer

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“…Indeed, 15 alternative human Oct-1 (POU2F1) transcripts are annotated in The Genome Annotation for Alternative Splicing Database (http://genome.ewha.ac.kr/ECgene). Previously, we studied Oct-1A, Oct-1L, and Oct-1X and have demonstrated that they start from alternative promoters of the POU2F1 gene and the predicted isoforms differ by their N-termini (27–29). At present time, the ‘canonical’ Oct-1 protein is considered to be a sequence of 743 aa corresponding to Oct-1X (UniProt, P14859-1).…”

Section: Introductionmentioning

confidence: 99%

Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

et al. 2016

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Oct-1 transcription factor has various functions in gene regulation. Its expression level is increased in several types of cancer and is associated with poor survival prognosis. Here we identified distinct Oct-1 protein isoforms in human cells and compared gene expression patterns and functions for Oct-1A, Oct-1L, and Oct-1X isoforms that differ by their N-terminal sequences. The longest isoform, Oct-1A, is abundantly expressed and is the main Oct-1 isoform in most of human tissues. The Oct-1L and the weakly expressed Oct-1X regulate the majority of Oct-1A targets as well as additional sets of genes. Oct-1X controls genes involved in DNA replication, DNA repair, RNA processing, and cellular response to stress. The high level of Oct-1 isoforms upregulates genes related to cell cycle progression and activates proliferation both in Namalwa Burkitt's lymphoma cells and primary human fibroblasts. It downregulates expression of genes related to antigen processing and presentation, cytokine-cytokine receptor interaction, oxidative metabolism, and cell adhesion, thus facilitating pro-oncogenic processes.

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The regulation of the Oct-1 gene transcription is mediated by two promoters

Cited by 15 publications

References 29 publications

LOX-1 Transcription

LOX-1 Transcription

OCT4B expression in PBMNCs suggests the existence of an alternative OCT4 promoter

Different N-terminal isoforms of Oct-1 control expression of distinct sets of genes and their high levels in Namalwa Burkitt's lymphoma cells affect a wide range of cellular processes

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