The regulation of tendon stem cell differentiation by the alignment of nanofibers

Yin, Zi; Chen, Xiao; Chen, Jia Lin; Shen, Wei; Nguyen, Thi Minh Hieu; Gao, Ling

doi:10.1016/j.biomaterials.2009.11.083

Cited by 535 publications

(499 citation statements)

References 44 publications

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“…The isolation and culture protocols for rabbit MeSCs were based on previous studies [14,20]. Meniscus tissues of New Zealand white (NZW) rabbits were digested with collagenase (3 mg/mL) for 6 h. The cells from the digested tissue were cultured in Dulbecco's modified Eagle's medium (DMEM, low glucose; Gibco-BRL, Inc.) supplemented with penicillin-streptomycin and 20% (v/v) fetal bovine serum (FBS; Invitrogen, Inc.).…”

Section: Monoclonal Selection Of Mescs and Colony Forming Unit Assaymentioning

confidence: 99%

“…The multipotent differentiation potential of MeSCs toward the adipogenic, osteogenic, and chondrogenic lineages was evaluated in vitro according to established protocols [20]. For adipogenic differentiation, MeSCs were induced under the influence of 1-methyl-3-isobutylxanthine, dexamethasone, insulin, and indomethacin.…”

Section: Multipotent Differentiation Potential Of Mescsmentioning

confidence: 99%

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Osteoarthritis Prevention Through Meniscal Regeneration Induced by Intra-Articular Injection of Meniscus Stem Cells

Shen

Chen

Zhu

et al. 2013

Stem Cells and Development

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Meniscus injury is frequently encountered in clinical practice. Current surgical therapy involving partial or complete meniscectomy relieves pain in the short-term but often leads to osteoarthritis (OA) in the long-term. Here, this study aimed to identify and characterize a novel population of meniscus-derived stem cells (MeSCs) and develop a new strategy of articular cartilage protection by intra-articular injection of these cells. The ''stemness'' and immune properties of MeSCs were investigated in vitro, while the efficacy of intra-articular injection of MeSCs for meniscus regeneration and OA prevention were investigated in vivo at 4, 8, and 12 weeks postsurgery. MeSCs displayed typical stem cell characteristics such as low immunogenicity and even possessed immunosuppressive function. In a rabbit meniscus injury model, transplantation of allogenous MeSCs did not elicit immunological rejection, but promoted neo-tissue formation with better-defined shape and more matured extracellular matrix. In a rabbit experimental OA model, transplantation of MeSCs further protected joint surface cartilage and maintained joint space at 12 weeks postsurgery, whereas extensive joint surface irregularities and joint space stenosis were observed in the control group. This study thus evoked a new strategy for articular cartilage protection and meniscus regeneration by intra-articular injection of MeSCs for patients undergoing meniscectomy.

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Section: Monoclonal Selection Of Mescs and Colony Forming Unit Assaymentioning

confidence: 99%

Section: Multipotent Differentiation Potential Of Mescsmentioning

confidence: 99%

Osteoarthritis Prevention Through Meniscal Regeneration Induced by Intra-Articular Injection of Meniscus Stem Cells

Shen

Chen

Zhu

et al. 2013

Stem Cells and Development

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“…electro-spinning [26][27][28][29][30][31], fibre extrusion [32][33][34][35], isoelectric focusing [36,37] and imprinting [38][39][40]) have been at the forefront of scientific and technological research and innovation to recapitulate native tendon extracellular matrix (ECM) supramolecular assemblies. Although fibrous constructs (e.g.…”

Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

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“…Rabbit tendon stem/progenitor cells (rTSPCs) were isolated as described previously [14]. Briefly, rotator cuff tendons of female rabbits (6 months old) were collected, washed in phosphatebuffered saline (PBS), and cut into 1-2 mm 3 pieces.…”

Section: Cell Seeding In Scaffoldsmentioning

confidence: 99%

“…Recent studies showed that aligned scaffolds can exert a profound influence on cell morphology and tendon-related gene expression, resulting in enhancement of tenogenic differentiation both in vitro and in vivo with animal injury models [14][15][16][17]. However, aligned scaffolds fabricated by the electrospinning procedure are nanoporous, usually having low porosity with small pores between fibers.…”

Section: Introductionmentioning

confidence: 99%

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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a b s t r a c tRotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, while commercially-available synthetic scaffolds are often inadequate at inducing tenogenic differentiation. The aim of this study is to develop an advanced 3D aligned collagen/silk scaffold (ACS) and investigate its efficacy in a rabbit massive rotator cuff tear model. ACS has similar 3D alignment of collagen fibers as natural tendon with superior mechanical characteristics. Based on ectopic transplantation studies, the optimal collagen concentration (10 mg/ml), pore diameter (108.43 ± 7.25 lm) and porosity (97.94 ± 0.08%) required for sustaining a stable macro-structure conducive for cellular infiltration was determined. Within in vitro culture, tendon stem/progenitor cells (TSPCs) displayed spindle-shaped morphology, and were well-aligned on ACS as early as 24 h. TSPCs formed intercellular contacts and deposited extracellular matrix after 7 days. With the in vivo rotator cuff repair model, the regenerative tendon of the ACS group displayed more conspicuous native microstructures with larger diameter collagen fibrils (48.72 ± 3.75 vs. 44.26 ± 5.03 nm) that had better alignment and mechanical properties (139.85 ± 49.36 vs. 99.09 ± 33.98 N) at 12 weeks post-implantation. In conclusion, these findings demonstrate the positive efficacy of the macroporous 3D aligned scaffold in facilitating rotator cuff tendon regeneration, and its practical applications for rotator cuff tendon tissue engineering. Statement of SignificanceMassive rotator cuff tear is one of the most common shoulder injuries, and poses a formidable clinical challenge to the orthopedic surgeon. Tissue engineering of tendon can potentially overcome the problem. However, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for repairing massive rotator cuff tendon injuries need to be developed. In this study, we developed a novel macroporous 3D aligned collagen/silk scaffold, and demonstrated that this novel scaffold enhanced the efficacy of rotator cuff tendon regeneration by inducing aligned supracellular structures similar to natural tendon, which in turn enhanced http://dx

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The regulation of tendon stem cell differentiation by the alignment of nanofibers

Cited by 535 publications

References 44 publications

Osteoarthritis Prevention Through Meniscal Regeneration Induced by Intra-Articular Injection of Meniscus Stem Cells

Osteoarthritis Prevention Through Meniscal Regeneration Induced by Intra-Articular Injection of Meniscus Stem Cells

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

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