The regulation of steroidogenesis by opioid peptides in porcine theca cells

Kamiński, Tadeusz; Siawrys, Gabriela; Bogacka, Iwona; Okrasa, S; Przala, J

doi:10.1016/s0378-4320(03)00042-3

Cited by 32 publications

(24 citation statements)

References 41 publications

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“…They affect the reproductive axis in females at all levels. In studies performed in vitro, with the use of porcine tissues/cells, selected opioid agonists appeared to influence gonadotropin releasing hormone release from the stalk median eminence (Okrasa et al, 1995), gonadotropin secretion by pituitary cells (Wylot et al, 2013) and steroid hormones by ovarian cells (Kaminski et al, 2003(Kaminski et al, , 2004. It was also reported that EOPs may affect many different processes in the uterus, including: cell proliferation (Vértes et al, 1996), apoptosis (Chatzaki et al, 2001), immunological interactions and myometrial contractility (Zoumakis et al, 1997) as well as the early pregnancy events (Li et al, 1992(Li et al, , 1993.…”

Section: Introductionmentioning

confidence: 99%

The expression of mRNAs for opioid precursors in endometrium of cyclic and early pregnant pigs; effects of IL-1β, IL-6 and TNFα

Dziekoński¹,

Zmijewska²,

Franczak³

et al. 2015

J. Anim. Feed Sci.

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Section: Introductionmentioning

confidence: 99%

The expression of mRNAs for opioid precursors in endometrium of cyclic and early pregnant pigs; effects of IL-1β, IL-6 and TNFα

Dziekoński¹,

Zmijewska²,

Franczak³

et al. 2015

J. Anim. Feed Sci.

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“…In porcine granulosa cells, however, it was demonstrated that the activation of PI hydrolysis and the consequential accumulation of IPs stimulated steroidogenesis (Sadighian et al, 1989). Moreover, the inhibition of PI hydrolysis suppressed steroid hormone secretion by porcine theca cells (Kaminski et al, 2003). The accumulation of IPs was also associated with an increase in P 4 production in luteal cells of primates and pigs (Ciereszko et al, 1995;Kaminski et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

Franczak

Kurowicka

Kowalik

et al. 2009

Acta Veterinaria Hungarica

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Oxytocin (OT) is involved in the regulation of steroid secretion by the corpus luteum (CL) in pigs, but OT signal transduction in the porcine CL has not been identified. In this study, the effects of OT on in vitro progesterone (P 4 ) secretion, phosphoinositide (PI) hydrolysis and intracellular mobilisation of Ca 2+ ([Ca 2+ ] i ) were investigated in porcine luteal cells during the early (days 3-5), mid-(days 8-10) and late luteal phases (days 12-14) of the oestrous cycle. Basal concentrations of P 4 and accumulation of inositol phosphates (IPs) were higher (P < 0.05) on days 3-5 and 8-10 of the oestrous cycle than on days 12-14. Basal [Ca 2+ ] i mobilisation did not differ among studied periods of the oestrous cycle. Oxytocin (10 -7 M) enhanced P 4 secretion and PI hydrolysis (P < 0.05) by luteal cells harvested on days 8-10 of the oestrous cycle. Moreover, OT started to increase mobilisation of [Ca 2+ ] i at the 15th (days 3-5 and 8-10) or 30th second (days 12-14) in porcine luteal cells. It was concluded that in pigs OT acts as a regulator of steroidogenesis, stimulating P 4 secretion in mature CL. This OT action may be mediated by changes in PI hydrolysis and [Ca 2+ ] i mobilisation.

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“…The granulosa cells were aspirated with a syringe and additionally washed out with a strong stream of media directed to the internal wall of the follicle (Kaminski et al, 2004). Granulosa cells were scraped from theca interna layer and theca cells were enzymatically dispersed in 0.25% trypsin solution (Kaminski et al, 2003). Dispersed cells were centrifuged (800 × g for 10 min) and washed twice or three times for granulosa and theca interna cells, respectively.…”

Section: Isolation Of Granulosa and Theca Interna Cellsmentioning

confidence: 99%

Expression of adiponectin receptors 1 and 2 in the ovary and concentration of plasma adiponectin during the oestrous cycle of the pig

Maleszka

Smolińska

Nitkiewicz

et al. 2014

Acta Veterinaria Hungarica

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The aim of this study was to compare the expression levels of adiponectin receptor 1 and adiponectin receptor 2 mRNAs and proteins in porcine ovaries during four stages (days 2 to 3, 10 to 12, 14 to 16, 17 to 19) of the oestrous cycle and to measure adiponectin plasma concentrations during the same phases of the cycle. Higher mRNA expression of adiponectin receptor 1 was detected in porcine granulosa cells than in corpora lutea and theca cells (P < 0.01). In contrast, higher gene expression of adiponectin receptor 2 occurred in newly developed and mature corpora lutea (P < 0.01). The adiponectin receptor 1 protein content was the highest in corpora lutea isolated on days 2 to 3 of the cycle and was the lowest in theca interna cells (P < 0.01). The profile of adiponectin receptor 2 protein was similar to that of adiponectin receptor 1. Adiponectin plasma concentrations were significantly higher throughout the luteal phase than in the follicular phase (P < 0.01). In conclusion, the presence of adiponectin receptor 1 and adiponectin receptor 2 mRNAs and proteins in the porcine ovary suggests that adiponectin may directly affect ovarian functions through its own specific receptors. The expression of both receptors and adiponectin plasma concentration were dependent on hormonal status related to the stage of the cycle. Key words: Adiponectin receptors, adiponectin, pig, ovary, oestrous cycleAdiponectin is an adipose-secreted 244-amino-acid protein with 30 kDa molecular weight, which circulates in the blood in the form of a trimer (low molecular weight, LMW), a hexamer (middle molecular weight, MMW) or a multimer (high molecular weight, HMW) (Maeda et al., 1996). Adiponectin exerts its effect by binding to two distinct but structurally related 7-transmembrane receptors, adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2).

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The regulation of steroidogenesis by opioid peptides in porcine theca cells

Cited by 32 publications

References 41 publications

The expression of mRNAs for opioid precursors in endometrium of cyclic and early pregnant pigs; effects of IL-1β, IL-6 and TNFα

The expression of mRNAs for opioid precursors in endometrium of cyclic and early pregnant pigs; effects of IL-1β, IL-6 and TNFα

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

Expression of adiponectin receptors 1 and 2 in the ovary and concentration of plasma adiponectin during the oestrous cycle of the pig

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