The regulation of stem cell differentiation by cell-cell contact on micropatterned material surfaces

Tang, Jian; Peng, Rong; Ding, Jiandong

doi:10.1016/j.biomaterials.2009.12.006

Cited by 299 publications

(252 citation statements)

References 60 publications

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“…The sporadic mineralization and random adipogenic differentiation in control cultures on day 21 may be explained by increased cell numbers and cellular contacts as well as paracrine signaling (Tang et al 2010;Zhurova et al 2010). Several cell signaling molecules, including BMPs and insulin growth factor, have been proposed to display pro-osteogenic and proadipogenic effects (James 2013).…”

Section: Discussionmentioning

confidence: 99%

Growth Factor Liberation and DPSC Response Following Dentine Conditioning

et al. 2016

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Liberation of the sequestrated bioactive molecules from dentine by the action of applied dental materials has been proposed as an important mechanism in inducing a dentinogenic response in teeth with viable pulps. Although adhesive restorations and dentinebonding procedures are routinely practiced, clinical protocols to improve pulp protection and dentine regeneration are not currently driven by biological knowledge. This study investigated the effect of dentine (powder and slice) conditioning by etchants/conditioners relevant to adhesive restorative systems on growth factor solubilization and odontoblast-like cell differentiation of human dental pulp progenitor cells (DPSCs). The agents included ethylenediaminetetraacetic acid (EDTA; 10%, pH 7.2), phosphoric acid (37%, pH <1), citric acid (10%, pH 1.5), and polyacrylic acid (25%, pH 3.9). Growth factors were detected in dentine matrix extracts drawn by EDTA, phosphoric acid, and citric acid from powdered dentine. The dentine matrix extracts were shown to be bioactive, capable of stimulating odontogenic/osteogenic differentiation as observed by gene expression and phenotypic changes in DPSCs cultured in monolayer on plastic. Polyacrylic acid failed to solubilize proteins from powdered dentine and was therefore considered ineffective in triggering a growth factor-mediated response in cells. The study went on to investigate the effect of conditioning dentine slices on growth factor liberation and DPSC behavior. Conditioning by EDTA, phosphoric acid, and citric acid exposed growth factors on dentine and triggered an upregulation in genes associated with mineralized differentiation, osteopontin, and alkaline phosphatase in DPSCs cultured on dentine. The cells demonstrated odontoblast-like appearances with elongated bodies and long extracellular processes extending on dentine surface. However, phosphoric acid-treated dentine appeared strikingly less populated with cells, suggesting a detrimental impact on cell attachment and growth when conditioning by this agent. These findings take crucial steps in informing clinical practice on dentineconditioning protocols as far as treatment of operatively exposed dentine in teeth with vital pulps is concerned.

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Section: Discussionmentioning

confidence: 99%

Growth Factor Liberation and DPSC Response Following Dentine Conditioning

et al. 2016

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“…67,68 Tang et al recently demonstrated that there is a linear relationship between osteogenic differentiation potential and the number of cell-cell contacts. 69 As a result, nanostructured surfaces, which are able to support cell proliferation, may indirectly favor cell differentiation by promoting cell-cell contact.…”

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confidence: 99%

Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

Peppo¹,

Agheli²,

Karlsson³

et al. 2014

IJN

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Background Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices. Methods In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration.

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“…It has been demonstrated that cell-cell contacts provide additional signaling to improve adipogenic and osteogenic differentiation, 67 through the increased gap junction or cadherin signaling. 59,90 In chondrogenic differentiation, the widely used micromass culture induces an artificial condensation event and creates a cellular microenvironment that promotes the formation of cartilage tissue in response to a chondrogenic cytokine milieu. 75 Additionally, cell-cell contact in the aggregates is required to activate Notch signaling to initiate chondrogenesis.…”

mentioning

confidence: 99%

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Sart

Tsai

et al. 2014

Tissue Engineering Part B: Reviews

326

362

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Mesenchymal stem cells (MSCs) are primary candidates in cell therapy and tissue engineering and are being tested in clinical trials for a wide range of diseases. Originally isolated and expanded as plastic adherent cells, MSCs have intriguing properties of in vitro self-assembly into three-dimensional (3D) aggregates reminiscent of skeletal condensation in vivo. Recent studies have shown that MSC 3D aggregation improved a range of biological properties, including multilineage potential, secretion of therapeutic factors, and resistance against ischemic condition. Hence, the formation of 3D MSC aggregates has been explored as a novel strategy to improve cell delivery, functional activation, and in vivo retention to enhance therapeutic outcomes. This article summarizes recent reports of MSC aggregate self-assembly, characterization of biological properties, and their applications in preclinical models. The cellular and molecular mechanisms underlying MSC aggregate formation and functional activation are discussed, and the areas that warrant further investigation are highlighted. These analyses are combined to provide perspectives for identifying the controlling mechanisms and refining the methods of aggregate fabrication and expansion for clinical applications.

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The regulation of stem cell differentiation by cell-cell contact on micropatterned material surfaces

Cited by 299 publications

References 60 publications

Growth Factor Liberation and DPSC Response Following Dentine Conditioning

Growth Factor Liberation and DPSC Response Following Dentine Conditioning

Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

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