The regulation of mitochondrial substrate utilization during acute exercise

Petrick, Heather L.; Holloway, Graham P.

doi:10.1016/j.cophys.2019.04.021

Cited by 4 publications

(7 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is likely that mitochondrial pyruvate transport and PDH capacity are not primarily responsible for the changes in substrate metabolism after OVX. Of note, PDH activity is allosterically regulated by posttranslational modifications (39,41), suggesting that protein level of total PDH is not reflective of substrate flux in vivo during exercise. Indeed, we found here that endurance training enhanced total PDH protein content in the plantaris and soleus muscles, despite lower RER during endurance exercise.…”

Section: Discussionmentioning

confidence: 99%

“…Mitochondrial substrate utilization is potentially determined by pyruvate influx into mitochondria and flux through PDH to produce acetyl-CoA from pyruvate, because acetyl-CoA is an intersection substrate between carbohydrates and fat (39). Mitochondrial pyruvate transport is facilitated, in part, by VDAC at the outer membrane, and MPC1 and MPC2 at the inner membrane (40).…”

Section: Discussionmentioning

confidence: 99%

“…These data suggest that the mitochondrial enzymatic capacity is not always indicative of fatty acid oxidation in vivo. Instead, the mitochondrial function may be important for substrate oxidation, including fat oxidation (39). Additional mechanisms for greater fat oxidation involve fatty acid availability due to higher lipolytic sensitivity to catecholamines in adipose tissue (53,54) and hormone-sensitive lipase protein abundance in the skeletal muscle (55).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Endurance Training on Metabolic Enzyme Activity and Transporter Proteins in Skeletal Muscle of Ovariectomized Mice

Takahashi

Kitaoka

Matsunaga

et al. 2022

Medicine &Amp; Science in Sports &Amp; Exercise

View full text Add to dashboard Cite

PurposeEstrogen deficiency or insufficiency can occur under several conditions, leading to negative health outcomes. To establish an effective countermeasure against estrogen loss, we investigated the effects of endurance training on ovariectomy (OVX)-induced metabolic disturbances.MethodsFemale Institute of Cancer Research mice underwent OVX or sham operations. On day 7 of recovery, the mice were randomized to remain either sedentary or undergo 5 wk of treadmill running (15–20 m·min−1, 60 min, 5 d·wk−1). During week 5 of the training, all animals performed a treadmill running test (15 m·min−1, 60 min).ResultsAfter the experimental period, OVX resulted in greater gains in body mass, fat mass, and triglyceride content in the gastrocnemius muscle. OVX enhanced phosphofructokinase activity in the plantaris muscle and decreased lactate dehydrogenase activity in the plantaris and soleus muscles. OVX decreased the protein content of NDUFB8, a mitochondrial respiratory chain subunit, but did not decrease other mitochondrial proteins or enzyme activities. Endurance training significantly enhanced mitochondrial enzyme activity and protein content in the skeletal muscles. Although OVX increased the respiratory exchange ratio during the treadmill running test, and postexercise blood lactate levels, endurance training normalized these parameters.ConclusionsThe present findings suggest that endurance training is a viable strategy to counteract the negative metabolic consequences in hypoestrogenism.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Endurance Training on Metabolic Enzyme Activity and Transporter Proteins in Skeletal Muscle of Ovariectomized Mice

Takahashi

Kitaoka

Matsunaga

et al. 2022

Medicine &Amp; Science in Sports &Amp; Exercise

View full text Add to dashboard Cite

show abstract

“…Furthermore, while ACC phosphorylation is blunted in mice with muscle-specific AMPKα deletion, skeletal muscle palmitate oxidation is not decreased during contraction compared to wild-type controls (Hingst et al 2020). In support of this evidence against a role of the AMPK-ACC-MCoA axis in regulating FAO during exercise, it is well accepted that MCoA content is not reduced during exercise in human skeletal muscle, despite an increase in FAO (Petrick & Holloway, 2019), suggesting mechanisms independent of MCoA content regulate FAO. In this respect, the sensitivity of CPT-1 to MCoA inhibition is acutely decreased during exercise, and the exercise-mediated increase in palmitoyl-CoA availability can further override the inhibitory effects of MCoA (Petrick & Holloway, 2019).…”

Section: The Role Of Ampk Activation During Acute Exercisementioning

confidence: 79%

Skeletal muscle AMPK activation: mounting evidence against a role in substrate utilization during acute exercise

Frangos

DesOrmeaux

Petrick

2020

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

“…In the present study, although we observed a tendency to increase complex I enzyme activity and a significant increase in complex I + III enzyme activity after lactate administration, neither MOc3 nor MOc2 respiration was changed. While both pyruvate and fatty acids are converted to acetyl-CoA and NADH, the maximal flux through PDH to produce these metabolites from pyruvate is much higher than the reaction of fatty acid oxidation ( Petrick and Holloway 2019 ). These observations suggest that NADH production through fatty acid oxidation did not reach the maximal capacity of complex I to consume NADH, and that reactions other than NADH consumption are key events for fatty acid-induced mitochondrial respiration.…”

Section: Discussionmentioning

confidence: 99%

Effects of Lactate Administration on Mitochondrial Respiratory Function in Mouse Skeletal Muscle

Takahashi

Tamura²,

Kitaoka³

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

Recent evidence has shown that mitochondrial respiratory function contributes to exercise performance and metabolic health. Given that lactate is considered a potential signaling molecule that induces mitochondrial adaptations, we tested the hypothesis that lactate would change mitochondrial respiratory function in skeletal muscle. Male ICR mice (8 weeks old) received intraperitoneal injection of PBS or sodium lactate (1 g/kg BW) 5 days a week for 4 weeks. Mitochondria were isolated from freshly excised gastrocnemius muscle using differential centrifugation and were used for all analyses. Lactate administration significantly enhanced pyruvate + malate- and glutamate + malate-induced (complex I-driven) state 3 (maximal/ATP synthesis-coupled) respiration, but not state 2 (basal/proton conductance) respiration. In contrast, lactate administration significantly decreased succinate + rotenone-induced (complex II-driven) state 3 and 2 respiration. No significant differences were observed in malate + octanoyl-l-carnitine-induced state 3 or 2 respiration. The enzymatic activity of complex I was tended to increase and those of complexes I + III and IV were significantly increased after lactate administration. No differences were observed in the activities of complexes II or II + III. Moreover, lactate administration increased the protein content of NDUFS4, a subunit of complex I, but not those of the other components. The present findings suggest that lactate alters mitochondrial respiratory function in skeletal muscle.

show abstract

The regulation of mitochondrial substrate utilization during acute exercise

Cited by 4 publications

References 48 publications

Effects of Endurance Training on Metabolic Enzyme Activity and Transporter Proteins in Skeletal Muscle of Ovariectomized Mice

Effects of Endurance Training on Metabolic Enzyme Activity and Transporter Proteins in Skeletal Muscle of Ovariectomized Mice

Skeletal muscle AMPK activation: mounting evidence against a role in substrate utilization during acute exercise

Effects of Lactate Administration on Mitochondrial Respiratory Function in Mouse Skeletal Muscle

Contact Info

Product

Resources

About