1974
DOI: 10.1016/0008-8749(74)90077-x
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The regulation of lymphotoxin release from stimulated human lymphocyte cultures: The requirement for continual mitogen stimulation

Abstract: Concanavalin A (Con A) activates nonimmune human lymphocytes in vitro to undergo transformation, Dh'A synthesis, and lymphotoxin (LT) secretion. LT secretion is inhibited (within minutes) when free and membrane-bound Con A are removed by washing or incubation with the competitive inhibitor, a-methyl mannoside. LT secretion can be reinitiated by addition of fresh Con A. While LT can be rapidly regulated, blast transformation and cellular D?r;A synthesis are under less restrictive control. Although they appear t… Show more

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Cited by 22 publications
(6 citation statements)
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“…Previous and present results substantiate that a polyclonal lectin signal triggers release, within 5 hr, of cell-lytic molecules accumulated in the intracellular pools of preactivated human iymphocytes (1)(2)(3). Extensive studies reveal the LT forms in these supernatants are heterogeneous in their size (14,15), cell-lytic capacity (3,11,16,17), and their ability to bind to different cell types in vitro (3,6,17).…”
Section: Discussionsupporting
confidence: 83%
“…Previous and present results substantiate that a polyclonal lectin signal triggers release, within 5 hr, of cell-lytic molecules accumulated in the intracellular pools of preactivated human iymphocytes (1)(2)(3). Extensive studies reveal the LT forms in these supernatants are heterogeneous in their size (14,15), cell-lytic capacity (3,11,16,17), and their ability to bind to different cell types in vitro (3,6,17).…”
Section: Discussionsupporting
confidence: 83%
“…The cultures were established under two basic experimental protocols: (l)"primary" activation, effected by adding Con-A up to 20 µg/ml to nonactivated cells, and (2) "secondary" stimulation of previously activated cells, which were temporarily deactivated. The latter procedure has been described in detail previously (3,4) . Briefly, after a 48-hr activation by incubation with Con-A at 20 µg/ml, the cells were washed free of Con-A by the addition of phosphate buffered saline (PBS, 0.15 M NaCl, 0.01 M PO, buffer, pH 7.2) containing 5 x 10-2 M a-methyl-mannoside (MAM, Sigma), a competitive inhibitor of Con-A.…”
Section: Methodsmentioning
confidence: 99%
“…Throughout the course of these experiments it had been noted that some of the cell cultures when cut back to a low density experienced a marked lag phase before exponential growth (Figs. 3,8,9,11,and 12). It has now been recognized that when cells remain in the stationary phase of growth for 24 or more hours before being resuspended a t a low cell density in fresh medium they undergo a lag phase before exponential growth occurs.…”
Section: 53mentioning
confidence: 99%