The regulation of glucose transporter (GLUT1) expression by the RNA binding protein HuR

Gantt, Kira R.; Cherry, Joy; Richardson, Melissa; Karschner, Vesna; Atasoy, Ulus; Pekala, Phillip H.

doi:10.1002/jcb.20950

Cited by 29 publications

(31 citation statements)

References 22 publications

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“…In the present study we focused on VEGF to confirm in vivo the pathway previously described in pericytes, however, we cannot exclude the involvement of other mRNAs that are targeted by HuR [60,61] and involved in angiogenesis as well, such as TNFα [62]. Even though we demonstrated the involvement of isoform beta of PKC, we cannot rule out the implication of other isoforms such as alpha and delta.…”

Section: Discussionmentioning

confidence: 55%

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

Amadio¹,

Bucolo²,

Leggio³

et al. 2010

Biochemical Pharmacology

100

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Section: Discussionmentioning

confidence: 55%

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

Amadio¹,

Bucolo²,

Leggio³

et al. 2010

Biochemical Pharmacology

100

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“…RNAs of three known targets of ELAVL1 regulation, Slc7a1, Gclc and Slc2a1 (Yaman et al 2002, Song et al 2005, Gantt et al 2006, which contain 3 0 UTR-like ARE sequences, all increased in expression at the blastocyst stage. Our data are consistent with detection of Gclc mRNA and protein only at the mouse blastocyst stage (Stover et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Three RBP target mRNAs were selected, as all are identified as targets of ELAVL1 in other tissues (Yaman et al 2002, Song et al 2005, Gantt et al 2006. g-Glutamylcysteine ligase catalytic unit (GCLC) is a key enzyme involved in synthesis of glutathione, which reacts to cellular stresses and deactivates destructive reactive oxygen species (Meister 1994).…”

Section: Introductionmentioning

confidence: 99%

Culture medium, gas atmosphere and MAPK inhibition affect regulation of RNA-binding protein targets during mouse preimplantation development

Calder¹,

Watson²,

Watson³

2011

Reproduction

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During oogenesis, mammalian oocytes accumulate maternal mRNAs that support the embryo until embryonic genome activation. RNA-binding proteins (RBP) may regulate the stability and turnover of maternal and embryonic mRNAs. We hypothesised that varying embryo culture conditions, such as culture medium, oxygen tension and MAPK inhibition, affects regulation of RBPs and their targets during preimplantation development. STAU1, ELAVL1, KHSRP and ZFP36 proteins and mRNAs were detected throughout mouse preimplantation development, whereas Elavl2 mRNA decreased after the two-cell stage. Potential target mRNAs of RBP regulation, Gclc, Slc2a1 and Slc7a1 were detected during mouse preimplantation development. Gclc mRNA was significantly elevated in embryos cultured in Whitten's medium compared with embryos cultured in KSOMaa, and Gclc mRNA was elevated under high-oxygen conditions. Inhibition of the p38 MAPK pathway reduced Slc7a1 mRNA expression while inhibition of ERK increased Slc2a1 mRNA expression. The half-lives of the potential RBP mRNA targets are not regulated in parallel; Slc2a1 mRNA displayed the longest half-life. Our results indicate that mRNAs and proteins encoding five RBPs are present during preimplantation development and more importantly, demonstrate that expression of RBP target mRNAs are regulated by culture medium, gas atmosphere and MAPK pathways.

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“…Studies are needed to define the cis and trans elements responsible for this inhibitory translational control. Gantt et al (21) showed that an RNA-binding protein (RBP), HuR, binds to glucose transporter 1 and leptin mRNA in adipocytes. We also detected HuR binding to leptin mRNA (our unpublished observation) and are presently examining whether this and other RBPs regulate leptin mRNA translation or stability and whether nutritional or hormonal status can affect their binding to leptin mRNA.…”

Section: Mechanisms Regulating Leptin Translationmentioning

confidence: 99%

Integration of hormonal and nutrient signals that regulate leptin synthesis and secretion

Lee¹,

Fried²

2009

American Journal of Physiology-Endocrinology and Metabolism

113

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Lee M-J, Fried SK. Integration of hormonal and nutrient signals that regulate leptin synthesis and secretion. Am J Physiol Endocrinol Metab 296: E1230 -E1238, 2009. First published March 24, 2009 doi:10.1152/ajpendo.90927.2008.-This review summarizes recent advances in our understanding of the pre-and posttranscriptional mechanisms that regulate leptin production and secretion in adipocytes. Basal leptin production is proportional to the status of energy stores, i.e., fat cell size, and this is mainly regulated by alterations in leptin mRNA levels. Leptin mRNA levels are regulated by hormones, including glucocorticoids and catecholamines, but little is known about the transcriptional mechanisms involved. Leptin synthesis and secretion is also acutely modulated in response to hormones such as insulin and the availability of metabolic fuels. Acute variations in leptin production over a time course of minutes to hours are mediated at the levels of both translation and secretion. Increases in amino acids and insulin after a meal activate the mammalian target of rapamycin (mTOR) pathway, leading to an increase in specific rates of leptin biosynthesis. Cross-talk among mTOR, PKA, and AMPactivated protein kinase pathways appears to integrate hormonal and nutrient signals that regulate leptin mRNA translation, at least in part through mechanisms involving its 5Ј-and 3Ј-untranslated regions. In addition, the rate of leptin secretion from preformed stores in response to hormonal cues is also regulated. Insulin stimulates, and adrenergic agonists inhibit, leptin secretion, and this likely contributes to variations in the magnitude of nutrition-related leptin excursions and oscillations. Overall, the study of leptin production has contributed to a deepening understanding of leptin biology and, more broadly, to our understanding of the cellular and molecular mechanisms by which the adipocyte integrates hormonal and nutrient signals to regulate adipokine production. adipose tissue; adrenergic; feeding; insulin; obesity LEPTIN IS A 16-KDA PROTEIN encoded by the obese (lep) gene that is expressed and secreted mainly by adipocytes. When energy stores are low, a fall in leptin decreases thermogenesis, promotes fatty acid (FA) over glucose oxidation, and decreases the activity of the hypothalamic pituitary adrenal and gonadal axes (1). Leptin also modulates the activities of the hematopoietic (5) and immune (52) systems, as well as angiogenesis (7).

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The regulation of glucose transporter (GLUT1) expression by the RNA binding protein HuR

Cited by 29 publications

References 22 publications

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

The PKCβ/HuR/VEGF pathway in diabetic retinopathy

Culture medium, gas atmosphere and MAPK inhibition affect regulation of RNA-binding protein targets during mouse preimplantation development

Integration of hormonal and nutrient signals that regulate leptin synthesis and secretion

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