The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses

Huo, Da; Sun, Lina; Storey, Kenneth B.; Zhang, Libin; Shi-lin, Liu; Sun, Jingjing; Yang, Hongsheng

doi:10.1016/j.scitotenv.2019.136045

Cited by 26 publications

(12 citation statements)

References 47 publications

(46 reference statements)

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“…Oyster could adapt hypoxia stress via metabolism depression and anaerobic glycolysis metabolism (Meng et al, 2018). In sea cucumbers LncRNA such as MSTRG.34610 and MSTRG.10941 could regulated HIF-1a gene and responed to hypoxia stress (Huo et al, 2020). In this study, we also dected LncMPEG1 in hypoxia stress under hight temperature and found that LncMPEG1 significant increase after 15d.…”

Section: Discussionmentioning

confidence: 62%

Long non-coding RNA LncMPEG1 responds to multiple environmental stressors by affecting biomineralization in pearl oyster Pinctada fucata martensii

Cai

Xie

et al. 2022

Front. Mar. Sci.

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Marine environmental change directly affects bivalve growth and survival. Exoskeleton formation, the main energy dissipation in the physiological metabolism, typically reflects the body growth of the bivalve. However, how bivalves regulate the biomineralization of the exoskeleton under environmental stressors is not yet clear. Long non-coding RNA regulates various life processes through complex mechanisms in vertebrates and invertebrates. In this research, we cloned the complete sequence of a mantle-specific expressed long non-coding RNA (designated as LncMPEG1) from a pearl oyster, Pinctada fucata martensii. A quantitative real-time PCR analysis showed that LncMPEG1 expression was significantly high in early umbo larvae and juveniles, which would be the critical periods of shell development. LncMPEG1 was identified in the outer epithelium of the middle fold from the mantle edge, mantle pallial, and mantle center by using in situ hybridization. Additionally, the expression of LncMPEG1 was stimulated by shell damage, alien invasion, heat and cold temperature stress, and hypoxia stress. In the mantle, a decreased in LncMPEG1 expression was detected by RNA interference, which can cause the irregular growth of crystals on the inner surface of the prismatic layer and nacre in the shells. Therefore, we propose that LncMPEG1 could be a key regulator in biomineralization and responds to environmental stress in the mantle .

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Section: Discussionmentioning

confidence: 62%

Long non-coding RNA LncMPEG1 responds to multiple environmental stressors by affecting biomineralization in pearl oyster Pinctada fucata martensii

Cai

Xie

et al. 2022

Front. Mar. Sci.

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“…*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 biological processes, including the response to environmental stress at both the transcriptional and post-transcriptional level (Dettleff et al 2020). Indeed, a previous study showed that environmental stress altered the expression levels of multiple lncRNAs and mRNAs, thus affecting various biological processes in A. japonicus, including immunity, energy metabolism, and the cell cycle (Huo et al 2020). Here, the co-expression relationship between lncRNA001074 and NKAα indicated that the cis-acting lncRNA001074 played critical roles in the response to low salt challenge (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…A growing body of evidence indicates that ncRNAs are also involved in various stress responses in aquatic animals (Liu et al 2020). LncRNAs could therefore play crucial roles in the regulation of defense responses to the thermal and hypoxic stresses associated with global climate change, by helping to maintain cell homeostasis and function (Huo et al 2020). Numerous ncRNAs associated with the regulation of gene expression in response to stress have been reported in sea cucumbers (Mu et al 2016;Tian et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Many reports have shown that salinity affects the expression of NKAα in many aquatic animals such as the Atlantic cod (Larsen et al 2012), southern flounder (Tipsmark et al 2008), Mozambique tilapia (Inokuchi et al 2008), and Atlantic salmon (McCormick et al 2008). Furthermore, salinity stress alters the expression profiles of multiple ncRNAs in many aquatic species (Huo et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Novel lncRNA lncRNA001074 participates in the low salinity–induced response in the sea cucumber Apostichopus japonicus by targeting the let-7/NKAα axis

Shang

Tian

Wang

et al. 2021

Cell Stress and Chaperones

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Salinity fluctuations have severe impacts on sea cucumbers and therefore important consequences in sea cucumber farming. The responses of sea cucumbers to salinity changes are reflected in the expression profiles of multiple genes and non-coding RNAs (ncRNAs). The microRNA (let-7) which is a developmental regulator, the ion transporter gene sodium potassium ATPase gene (NKAα), and the long ncRNA lncRNA001074 were previously shown to be involved in responses to salinity changes in various marine species. To better understand the relationship between ncRNAs and target genes, the let-7/NKAα/lncRNA001074 predicted interaction was investigated in this study using luciferase reporter assays and gene knockdowns in the sea cucumber Apostichopus japonicus. The results showed that NKAα was the target gene of let-7 and NKAα expression levels were inversely correlated with let-7 expression based on the luciferase reporter assays and western blots. The let-7 abundance was negatively regulated by lncRNA001074 and NKAα both in vitro and in vivo. Knockdown of lncRNA001074 led to let-7 overexpression. These results demonstrated that lncRNA001074 binds to the 3′-UTR binding site of let-7 in a regulatory manner. Furthermore, the expression profiles of let-7, NKAα, and lncRNA001074 were analyzed in sea cucumbers after the knockdown of each of these genes. The results found that lncRNA001074 competitively bound let-7 to suppress NKAα expression under low salinity conditions. The downregulation of let-7, in conjunction with the upregulation of lncRNA001074 and NKAα, may be essential for the response to low salinity change in sea cucumbers. Therefore, the dynamic balance of the lncRNA001074, NKAα, and let-7 network might be a potential response mechanism to salinity change in sea cucumbers.

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“…By the end of the century, it is expected that global temperatures will rise by at least 2°C, but ocean DO concentrations will drop by 4-7% (Matear and Hirst, 2003;Hoegh-Guldberg et al, 2007). Since oxygen becomes less soluble as temperature rises, heat stress and hypoxic stress frequently coexist (Huo et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Temperature and dissolved oxygen influence the immunity, digestion, and antioxidant level in sea cucumber Apostichopus japonicus

Huo

Zhang

et al. 2022

Front. Mar. Sci.

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The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses

Cited by 26 publications

References 47 publications

Long non-coding RNA LncMPEG1 responds to multiple environmental stressors by affecting biomineralization in pearl oyster Pinctada fucata martensii

Long non-coding RNA LncMPEG1 responds to multiple environmental stressors by affecting biomineralization in pearl oyster Pinctada fucata martensii

Novel lncRNA lncRNA001074 participates in the low salinity–induced response in the sea cucumber Apostichopus japonicus by targeting the let-7/NKAα axis

Temperature and dissolved oxygen influence the immunity, digestion, and antioxidant level in sea cucumber Apostichopus japonicus

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