The Regeneration Rate of the Water Vapour Loss of Heavily Damaged Skin

Spruit, D.; Malten, K. E.

doi:10.1159/000254410

Cited by 51 publications

(20 citation statements)

References 3 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Disruption of the permeability barrier was induced in hairless mice by tape stripping (Tesafilm ® , Beiersdorf, Hamburg, Germany) to remove cells from the stratum corneum, resulting in a superficial wound, until a 20-to 30-fold increase in transepidermal water loss (TEWL), as a marker of barrier disruption, was achieved (Meeco ® electronic water analyzer, Meeco ® Inc., Warrington PA) (Spruit and Malten, 1966;Grubauer et al, 1989). Barrier recovery was calculated as follows: Barrier recovery (%)=100 -(xh -baseline)100/(0h -baseline).…”

Section: Permeability Barrier Disruptionmentioning

confidence: 99%

Cathepsin D is involved in the regulation of transglutaminase 1 and epidermal differentiation

Egberts

Heinrich

Jensen

et al. 2004

Journal of Cell Science

114

View full text Add to dashboard Cite

We previously demonstrated that the aspartate protease cathepsin D is activated by ceramide derived from acid sphingomyelinase. Increased expression of cathepsin D in the skin has been reported in wound healing, psoriasis and skin tumors. We explored specific functions of cathepsin D during epidermal differentiation. Protein expression and enzymatic activity of cathepsin D increased in differentiated keratinocytes in both stratified organotypic cultures and in mouse skin during epidermal barrier repair. Treatment of cultured keratinocytes with exogenous cathepsin D increased the activity of transglutaminase 1, known to cross-link the cornified envelope proteins involucrin and loricrin during epidermal differentiation. Inhibition of cathepsin D by pepstatin A suppressed the activity of transglutaminase 1. Cathepsin D-deficient mice revealed reduced transglutaminase 1 activity and reduced protein levels of the cornified envelope proteins involucrin and loricrin. Also, amount and distribution of cornified envelope proteins involucrin, loricrin, filaggrin, and of the keratins K1 and K5 were significantly altered in cathepsin D-deficient mice. Stratum corneum morphology in cathepsin D-deficient mice was impaired, with increased numbers of corneocyte layers and faint staining of the cornified envelope only, which is similar to the human skin disease lamellar ichthyosis. Our findings suggest a functional link between cathepsin D activation, transglutaminase 1 activity and protein expression of cornified envelope proteins during epidermal differentiation.

show abstract

Section: Permeability Barrier Disruptionmentioning

confidence: 99%

Cathepsin D is involved in the regulation of transglutaminase 1 and epidermal differentiation

Egberts

Heinrich

Jensen

et al. 2004

Journal of Cell Science

114

View full text Add to dashboard Cite

show abstract

“…Physical stress includes occlusion, heat, electric pulse and tape stripping. Tape stripping is a common method to disrupt skin barrier function; the relationship between tape stripping and skin barrier function has been established [50,51] . Sequential stripping increases transepidermal water loss (TEWL), an indicator of water barrier defect, which correlates well with enhanced transdermal drug delivery [52] .…”

Section: Cers and Strippingmentioning

confidence: 99%

Role of Ceramides in Skin Stress: Ultraviolet Light, Tape Stripping and Crowding

Choi¹,

Maibach²

2003

Exog Dermatol

View full text Add to dashboard Cite

Stratum corneum intercellular lipids regulate skin water barrier function and water-holding capacity; their modification may impair these properties. Physical and chemical stresses diminish barrier function. Acute barrier disruption by tape stripping increases sphingomyelinase and serine palmitoyltransferase activity; ceramide contents are increased to restore barrier function. Overcrowding stress induces dry skin, and the barrier function impairment correlates with decreased skin cera- mides. The effect of UV irradiation on ceramide content and barrier function varies with doses and UV wavelength. Stress-induced ceramide generation may induce apoptosis in cultured human keratinocytes and restore barrier function. This review focuses on the role of ceramides in physical and chemical stress, suggesting that refinement and extension of this academic domain may lead to therapeutic advances.

show abstract

“…The sweat responses were followed continuously by use of the 'Meeco' electrolytic water vapour analyzer used in the fashion described by Spruit and Malten [4]. The prin ciple is that dry (< 5 ppm water) nitrogen gas is conducted via Teflon tubing to a 0.2 cm2 cup, applied lightly to the shaved skin surface.…”

Section: Experimental Sweat Responses To Various Drugsmentioning

confidence: 99%

Nature of the Sweat Glands in the Hairy Skin of the Beagle

Cotton¹,

Hasselt²,

Bergers³

1975

Dermatology

View full text Add to dashboard Cite

The local pharmacology, the thermal response and the response to hypothalamic stimulation of the sweat glands of the hairy surface of the beagle are described. The results, together with those of electron-microscopic examinations, support the idea that these sweat glands are apocrine and are not directly innervated. No clear relationship with thermoregulation could be found and a pheromonal function is tentatively suggested.

show abstract

The Regeneration Rate of the Water Vapour Loss of Heavily Damaged Skin

Cited by 51 publications

References 3 publications

Cathepsin D is involved in the regulation of transglutaminase 1 and epidermal differentiation

Cathepsin D is involved in the regulation of transglutaminase 1 and epidermal differentiation

Role of Ceramides in Skin Stress: Ultraviolet Light, Tape Stripping and Crowding

Nature of the Sweat Glands in the Hairy Skin of the Beagle

Contact Info

Product

Resources

About